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Protein

26S proteasome non-ATPase regulatory subunit 4

Gene

Psmd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 4
Alternative name(s):
26S proteasome regulatory subunit RPN10
26S proteasome regulatory subunit S5A
Multiubiquitin chain-binding protein
Gene namesi
Name:Psmd4
Synonyms:Mcb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1201670 Psmd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738291 – 37626S proteasome non-ATPase regulatory subunit 4Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei250PhosphothreonineCombined sources1
Modified residuei253PhosphothreonineCombined sources1
Modified residuei256PhosphoserineCombined sources1
Modified residuei360PhosphoserineBy similarity1
Isoform Rpn10B (identifier: O35226-2)
Modified residuei250PhosphothreonineCombined sources1
Modified residuei259PhosphoserineCombined sources1
Isoform Rpn10C (identifier: O35226-3)
Modified residuei250PhosphothreonineCombined sources1
Modified residuei259PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO35226
MaxQBiO35226
PaxDbiO35226
PeptideAtlasiO35226
PRIDEiO35226

2D gel databases

REPRODUCTION-2DPAGEiIPI00345779

PTM databases

iPTMnetiO35226
PhosphoSitePlusiO35226
SwissPalmiO35226

Expressioni

Tissue specificityi

Isoform Rpn10A is ubiquitous whereas isoform Rpn10E is mostly expressed in the embryonic brain.

Developmental stagei

Isoform Rpn10E is expressed only in the embryos.

Gene expression databases

BgeeiENSMUSG00000005625 Expressed in 300 organ(s), highest expression level in primary oocyte
ExpressionAtlasiO35226 baseline and differential
GenevisibleiO35226 MM

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4 (PubMed:16857966). Interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Interacts with UBE3A. Interacts with UBQLN1 (via ubiquitin-like domain).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202431, 5 interactors
IntActiO35226, 21 interactors
MINTiO35226
STRINGi10090.ENSMUSP00000102857

Structurei

3D structure databases

ProteinModelPortaliO35226
SMRiO35226
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 188VWFAPROSITE-ProRule annotationAdd BLAST184
Domaini211 – 230UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini282 – 301UIM 2PROSITE-ProRule annotationAdd BLAST20

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 262Interaction with UBQLN1By similarityAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi238 – 246Poly-Ala9

Domaini

The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.By similarity

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2884 Eukaryota
COG5148 LUCA
GeneTreeiENSGT00530000064050
HOGENOMiHOG000165630
HOVERGENiHBG000425
InParanoidiO35226
KOiK03029
OMAiEANTEEA
PhylomeDBiO35226
TreeFamiTF106232

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR003903 UIM_dom
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF02809 UIM, 2 hits
PF13519 VWA_2, 1 hit
SMARTiView protein in SMART
SM00726 UIM, 2 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 2 hits
PS50234 VWFA, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform Rpn10A (identifier: O35226-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG
60 70 80 90 100
LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR
110 120 130 140 150
QGKNHKMRII AFVGSPVEDN EKDLVKLAKR LKKEKVNVDI INFGEEEVNT
160 170 180 190 200
EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA DALISSPILA GEGGAMLGLG
210 220 230 240 250
ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA ASAAEAGIAT
260 270 280 290 300
PGTEDSDDAL LKMTINQQEF GRPGLPDLSS MTEEEQIAYA MQMSLQGTEF
310 320 330 340 350
SQESADMDAS SAMDTSDPVK EEDDYDVMQD PEFLQSVLEN LPGVDPNNAA
360 370
IRSVMGALAS QATKDGKNDK KEEEKK
Length:376
Mass (Da):40,704
Last modified:January 1, 1998 - v1
Checksum:i732AC02B56760EAA
GO
Isoform Rpn10B (identifier: O35226-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-254: E → EGER

Show »
Length:379
Mass (Da):41,046
Checksum:i9D3DC705FACF4AEE
GO
Isoform Rpn10C (identifier: O35226-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-254: E → EGER
     321-365: EEDDYDVMQD...GALASQATKD → VRASSEALTQ...KGLGATEGNT
     366-376: Missing.

Show »
Length:368
Mass (Da):39,590
Checksum:i1945E30F72EF6F3F
GO
Isoform Rpn10D (identifier: O35226-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     299-349: EFSQESADMD...NLPGVDPNNA → GGPWLGWQGL...QPWTHLIQSR
     350-376: Missing.

Show »
Length:349
Mass (Da):37,964
Checksum:iEE0CA045056F9A0F
GO
Isoform Rpn10E (identifier: O35226-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-260: DSDDAL → GERGGF
     261-376: Missing.

Show »
Length:260
Mass (Da):27,913
Checksum:iA715852C2CA04644
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7AI87F7AI87_MOUSE
26S proteasome non-ATPase regulator...
Psmd4
194Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005293254E → EGER in isoform Rpn10B and isoform Rpn10C. 1 Publication1
Alternative sequenceiVSP_005298255 – 260DSDDAL → GERGGF in isoform Rpn10E. Curated6
Alternative sequenceiVSP_005299261 – 376Missing in isoform Rpn10E. CuratedAdd BLAST116
Alternative sequenceiVSP_005296299 – 349EFSQE…DPNNA → GGPWLGWQGLDRVWGEELAL SGLLLSFPQSLAKNRLTWMP AQPWTHLIQSR in isoform Rpn10D. CuratedAdd BLAST51
Alternative sequenceiVSP_005294321 – 365EEDDY…QATKD → VRASSEALTQPSLTSPAFRS LSFWDQGLSSLAFHKKGLGA TEGNT in isoform Rpn10C. CuratedAdd BLAST45
Alternative sequenceiVSP_005297350 – 376Missing in isoform Rpn10D. CuratedAdd BLAST27
Alternative sequenceiVSP_005295366 – 376Missing in isoform Rpn10C. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013099 mRNA Translation: AAC53547.1
AB029090 Genomic DNA Translation: BAA97572.1
AB029142 mRNA Translation: BAA97573.1
AB029143 mRNA Translation: BAA97574.1
AB029144 mRNA Translation: BAA97575.1
AB029145 mRNA Translation: BAA97576.1
AB029146 mRNA Translation: BAA97577.1
AF175574 mRNA Translation: AAG09199.1
BC009005 mRNA Translation: AAH09005.1
CCDSiCCDS38541.1 [O35226-1]
CCDS71287.1 [O35226-2]
PIRiJC6535
RefSeqiNP_001268946.1, NM_001282017.1 [O35226-2]
NP_032977.1, NM_008951.2 [O35226-1]
UniGeneiMm.2261

Genome annotation databases

EnsembliENSMUST00000071664; ENSMUSP00000071589; ENSMUSG00000005625 [O35226-2]
ENSMUST00000107237; ENSMUSP00000102857; ENSMUSG00000005625 [O35226-1]
ENSMUST00000117355; ENSMUSP00000113554; ENSMUSG00000005625 [O35226-3]
GeneIDi19185
KEGGimmu:19185
UCSCiuc008qhr.2 mouse [O35226-1]
uc008qht.2 mouse [O35226-3]
uc008qhu.2 mouse [O35226-4]
uc008qhv.2 mouse [O35226-5]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013099 mRNA Translation: AAC53547.1
AB029090 Genomic DNA Translation: BAA97572.1
AB029142 mRNA Translation: BAA97573.1
AB029143 mRNA Translation: BAA97574.1
AB029144 mRNA Translation: BAA97575.1
AB029145 mRNA Translation: BAA97576.1
AB029146 mRNA Translation: BAA97577.1
AF175574 mRNA Translation: AAG09199.1
BC009005 mRNA Translation: AAH09005.1
CCDSiCCDS38541.1 [O35226-1]
CCDS71287.1 [O35226-2]
PIRiJC6535
RefSeqiNP_001268946.1, NM_001282017.1 [O35226-2]
NP_032977.1, NM_008951.2 [O35226-1]
UniGeneiMm.2261

3D structure databases

ProteinModelPortaliO35226
SMRiO35226
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202431, 5 interactors
IntActiO35226, 21 interactors
MINTiO35226
STRINGi10090.ENSMUSP00000102857

PTM databases

iPTMnetiO35226
PhosphoSitePlusiO35226
SwissPalmiO35226

2D gel databases

REPRODUCTION-2DPAGEiIPI00345779

Proteomic databases

EPDiO35226
MaxQBiO35226
PaxDbiO35226
PeptideAtlasiO35226
PRIDEiO35226

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071664; ENSMUSP00000071589; ENSMUSG00000005625 [O35226-2]
ENSMUST00000107237; ENSMUSP00000102857; ENSMUSG00000005625 [O35226-1]
ENSMUST00000117355; ENSMUSP00000113554; ENSMUSG00000005625 [O35226-3]
GeneIDi19185
KEGGimmu:19185
UCSCiuc008qhr.2 mouse [O35226-1]
uc008qht.2 mouse [O35226-3]
uc008qhu.2 mouse [O35226-4]
uc008qhv.2 mouse [O35226-5]

Organism-specific databases

CTDi5710
MGIiMGI:1201670 Psmd4

Phylogenomic databases

eggNOGiKOG2884 Eukaryota
COG5148 LUCA
GeneTreeiENSGT00530000064050
HOGENOMiHOG000165630
HOVERGENiHBG000425
InParanoidiO35226
KOiK03029
OMAiEANTEEA
PhylomeDBiO35226
TreeFamiTF106232

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-8951664 Neddylation
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiPsmd4 mouse
PROiPR:O35226
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005625 Expressed in 300 organ(s), highest expression level in primary oocyte
ExpressionAtlasiO35226 baseline and differential
GenevisibleiO35226 MM

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR003903 UIM_dom
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF02809 UIM, 2 hits
PF13519 VWA_2, 1 hit
SMARTiView protein in SMART
SM00726 UIM, 2 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 2 hits
PS50234 VWFA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD4_MOUSE
AccessioniPrimary (citable) accession number: O35226
Secondary accession number(s): Q91V59
, Q9JJM0, Q9JJM1, Q9JJM2, Q9JJM3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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