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Protein

Histone H3-like centromeric protein A

Gene

Cenpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division (By similarity). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:27499292).By similarity1 Publication

GO - Molecular functioni

  • nucleosomal DNA binding Source: GO_Central
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H3-like centromeric protein A
Alternative name(s):
Centromere protein A
Short name:
CENP-A
Gene namesi
Name:Cenpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88375 Cenpa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002213742 – 134Histone H3-like centromeric protein AAdd BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei22PhosphoserineBy similarity1
Modified residuei62PhosphoserineBy similarity1

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.
Trimethylated by NTMT1 at the N-terminal glycine after cleavage of Met-1. Methylation is low before incorporation into nucleosomes and increases with cell cycle progression, with the highest levels in mitotic nucleosomes.By similarity
Phosphorylated by CDK1 at Ser-62 during early mitosis; this abolishes association with chromatin and centromeres, prevents interaction with HJURP and thereby prevents premature assembly of CENPA into centromeres. Dephosphorylated at Ser-62 by PPP1CA during late mitosis.By similarity

Keywords - PTMi

ADP-ribosylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiO35216
PaxDbiO35216
PeptideAtlasiO35216
PRIDEiO35216

PTM databases

iPTMnetiO35216
PhosphoSitePlusiO35216

Expressioni

Gene expression databases

BgeeiENSMUSG00000029177 Expressed in 218 organ(s), highest expression level in urogenital fold
CleanExiMM_CENPA
ExpressionAtlasiO35216 baseline and differential
GenevisibleiO35216 MM

Interactioni

Subunit structurei

Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core. The octamer contains two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. CENPA modulates the DNA-binding characteristics of nucleosomes so that protruding DNA ends have higher flexibility than in nucleosomes containing conventional histone H3. Inhibits binding of histone H1 to nucleosomes, since histone H1 binds preferentially to rigid DNA linkers that protrude from nucleosomes. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Can assemble into nucleosomes that contain both CENPA and histone H3.3; these nucleosomes interact with a single CENPC chain. Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts (via CATD domain) with HJURP; the interaction is direct and is required for its localization to centromeres. Interacts with CENPC, CENPN and CENPT; interaction is direct. Part of a centromere complex consisting of CENPA, CENPT and CENPW. Identified in centromere complexes containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Can self-associate. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro). Interacts with CDK1, PPP1CA and RBBP7.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35216, 2 interactors
MINTiO35216
STRINGi10090.ENSMUSP00000122831

Structurei

3D structure databases

ProteinModelPortaliO35216
SMRiO35216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 48Important for flexibility of DNA ends that protrude from nucleosomesBy similarityAdd BLAST15
Regioni35 – 134H3-likeAdd BLAST100
Regioni69 – 110CATDAdd BLAST42

Domaini

The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA. It is necessary and sufficient to mediate the localization into centromeres.By similarity

Sequence similaritiesi

Belongs to the histone H3 family.Curated

Phylogenomic databases

eggNOGiKOG1745 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00910000144321
HOVERGENiHBG001172
InParanoidiO35216
KOiK11495
OMAiVHLFEDC
OrthoDBiEOG091G13T2
PhylomeDBiO35216
TreeFamiTF354293

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000164 Histone_H3/CENP-A
PANTHERiPTHR11426 PTHR11426, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00622 HISTONEH3
SMARTiView protein in SMART
SM00428 H3, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O35216-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPRRKPQTP RRRPSSPAPG PSRQSSSVGS QTLRRRQKFM WLKEIKTLQK
60 70 80 90 100
STDLLFRKKP FSMVVREICE KFSRGVDFWW QAQALLALQE AAEAFLIHLF
110 120 130
EDAYLLSLHA GRVTLFPKDI QLTRRIRGFE GGLP
Length:134
Mass (Da):15,509
Last modified:January 1, 1998 - v1
Checksum:i0381FA6492E43C3D
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JGI2A0A0G2JGI2_MOUSE
Histone H3-like centromeric protein...
Cenpa
99Annotation score:
A0A0G2JEV2A0A0G2JEV2_MOUSE
Centromere protein A, isoform CRA_f
Cenpa mCG_23505
130Annotation score:
D6RJ71D6RJ71_MOUSE
Centromere protein A, isoform CRA_a
Cenpa mCG_23505
39Annotation score:
D6RCV6D6RCV6_MOUSE
Centromere protein A, isoform CRA_c
Cenpa mCG_23505
42Annotation score:
A0A0G2JEV0A0A0G2JEV0_MOUSE
Histone H3-like centromeric protein...
Cenpa
59Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012709 mRNA Translation: AAC39957.1
AF012710 Genomic DNA Translation: AAC39958.1
AK010718 mRNA Translation: BAB27140.1
AK011399 mRNA Translation: BAB27591.1
AK053763 mRNA Translation: BAC35512.1
AK089033 mRNA Translation: BAC40710.1
BC011038 mRNA Translation: AAH11038.1
CCDSiCCDS19162.1
RefSeqiNP_001289058.1, NM_001302129.1
NP_001289059.1, NM_001302130.1
NP_001289060.1, NM_001302131.1
NP_001289061.1, NM_001302132.1
NP_031707.1, NM_007681.3
UniGeneiMm.290563

Genome annotation databases

EnsembliENSMUST00000144742; ENSMUSP00000122831; ENSMUSG00000029177
GeneIDi12615
KEGGimmu:12615
UCSCiuc008wvt.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012709 mRNA Translation: AAC39957.1
AF012710 Genomic DNA Translation: AAC39958.1
AK010718 mRNA Translation: BAB27140.1
AK011399 mRNA Translation: BAB27591.1
AK053763 mRNA Translation: BAC35512.1
AK089033 mRNA Translation: BAC40710.1
BC011038 mRNA Translation: AAH11038.1
CCDSiCCDS19162.1
RefSeqiNP_001289058.1, NM_001302129.1
NP_001289059.1, NM_001302130.1
NP_001289060.1, NM_001302131.1
NP_001289061.1, NM_001302132.1
NP_031707.1, NM_007681.3
UniGeneiMm.290563

3D structure databases

ProteinModelPortaliO35216
SMRiO35216
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35216, 2 interactors
MINTiO35216
STRINGi10090.ENSMUSP00000122831

PTM databases

iPTMnetiO35216
PhosphoSitePlusiO35216

Proteomic databases

MaxQBiO35216
PaxDbiO35216
PeptideAtlasiO35216
PRIDEiO35216

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144742; ENSMUSP00000122831; ENSMUSG00000029177
GeneIDi12615
KEGGimmu:12615
UCSCiuc008wvt.2 mouse

Organism-specific databases

CTDi1058
MGIiMGI:88375 Cenpa

Phylogenomic databases

eggNOGiKOG1745 Eukaryota
COG2036 LUCA
GeneTreeiENSGT00910000144321
HOVERGENiHBG001172
InParanoidiO35216
KOiK11495
OMAiVHLFEDC
OrthoDBiEOG091G13T2
PhylomeDBiO35216
TreeFamiTF354293

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:O35216
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029177 Expressed in 218 organ(s), highest expression level in urogenital fold
CleanExiMM_CENPA
ExpressionAtlasiO35216 baseline and differential
GenevisibleiO35216 MM

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007125 Histone_H2A/H2B/H3
IPR000164 Histone_H3/CENP-A
PANTHERiPTHR11426 PTHR11426, 1 hit
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PRINTSiPR00622 HISTONEH3
SMARTiView protein in SMART
SM00428 H3, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCENPA_MOUSE
AccessioniPrimary (citable) accession number: O35216
Secondary accession number(s): Q545C9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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