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Protein

Fractalkine

Gene

Cx3cl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (By similarity). The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).By similarity2 Publications

GO - Molecular functioni

  • CCR chemokine receptor binding Source: GO_Central
  • chemoattractant activity Source: MGI
  • chemokine activity Source: MGI
  • CX3C chemokine receptor binding Source: UniProtKB
  • CXCR1 chemokine receptor binding Source: MGI
  • integrin binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionCytokine
Biological processCell adhesion, Chemotaxis

Enzyme and pathway databases

ReactomeiR-MMU-380108 Chemokine receptors bind chemokines
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Fractalkine
Alternative name(s):
C-X3-C motif chemokine 1
CX3C membrane-anchored chemokine
Neurotactin
Small-inducible cytokine D1
Cleaved into the following chain:
Gene namesi
Name:Cx3cl1
Synonyms:Cx3c, Fkn, Scyd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1097153 Cx3cl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 336ExtracellularSequence analysisAdd BLAST312
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Topological domaini358 – 395CytoplasmicSequence analysisAdd BLAST38

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000525325 – 395FractalkineAdd BLAST371
ChainiPRO_000029622525 – ?337Processed fractalkineAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 58By similarity
Disulfide bondi36 ↔ 74By similarity

Post-translational modificationi

A soluble short 80 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei337 – 338Cleavage; to produce soluble formSequence analysis2

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO35188
PaxDbiO35188
PeptideAtlasiO35188
PRIDEiO35188

PTM databases

iPTMnetiO35188
PhosphoSitePlusiO35188

Expressioni

Tissue specificityi

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.3 Publications

Inductioni

By 12-O-tetradecanoylphorbol-13-acetate (TPA) and lipopolysaccharides (LPS) in bone marrow stroma cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000031778 Expressed in 225 organ(s), highest expression level in prefrontal cortex
CleanExiMM_CX3CL1
ExpressionAtlasiO35188 baseline and differential
GenevisibleiO35188 MM

Interactioni

Subunit structurei

Monomer. Forms a ternary complex with CX3CR1 and ITGAV:ITGB3 or ITGA4:ITGB1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35188, 1 interactor
MINTiO35188
STRINGi10090.ENSMUSP00000034230

Structurei

3D structure databases

ProteinModelPortaliO35188
SMRiO35188
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 100Chemokine and involved in interaction with ITGAV:ITGB3 and ITGA4:ITGB1By similarityAdd BLAST76
Regioni101 – 336Mucin-like stalkAdd BLAST236

Sequence similaritiesi

Belongs to the intercrine delta family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJQZ Eukaryota
ENOG41116BY LUCA
GeneTreeiENSGT00510000049483
HOGENOMiHOG000036946
HOVERGENiHBG057269
InParanoidiO35188
KOiK05508
OMAiYQSLQGC
OrthoDBiEOG091G0WNR
TreeFamiTF337534

Family and domain databases

CDDicd00274 Chemokine_CX3C, 1 hit
InterProiView protein in InterPro
IPR039809 Chemokine_b/g/d
IPR034127 Chemokine_CX3C
IPR001811 Chemokine_IL8-like_dom
IPR008097 CX3CL1
IPR036048 Interleukin_8-like_sf
PANTHERiPTHR12015 PTHR12015, 1 hit
PTHR12015:SF92 PTHR12015:SF92, 1 hit
PfamiView protein in Pfam
PF00048 IL8, 1 hit
SMARTiView protein in SMART
SM00199 SCY, 1 hit
SUPFAMiSSF54117 SSF54117, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O35188-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPSPLAWLL RLAAFFHLCT LLPGQHLGMT KCEIMCDKMT SRIPVALLIR
60 70 80 90 100
YQLNQESCGK RAIVLETTQH RRFCADPKEK WVQDAMKHLD HQAAALTKNG
110 120 130 140 150
GKFEKRVDNV TPGITLATRG LSPSALTKPE SATLEDLALE LTTISQEARG
160 170 180 190 200
TMGTSQEPPA AVTGSSLSTS EAQDAGLTAK PQSIGSFEAA DISTTVWPSP
210 220 230 240 250
AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENV GSEGQPPWVQ
260 270 280 290 300
GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELV
310 320 330 340 350
ASGSQAPKIE EPIHATADPQ KLSVLITPVP DTQAATRRQA VGLLAFLGLL
360 370 380 390
FCLGVAMFAY QSLQGCPRKM AGEMVEGLRY VPRSCGSNSY VLVPV
Length:395
Mass (Da):42,099
Last modified:July 27, 2011 - v3
Checksum:iB5A16F8BD4B80340
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D5RMK8A0A1D5RMK8_MOUSE
Fractalkine
Cx3cl1
64Annotation score:
D6RCV0D6RCV0_MOUSE
Fractalkine
Cx3cl1
57Annotation score:
A0A1D5RLW9A0A1D5RLW9_MOUSE
Fractalkine
Cx3cl1
45Annotation score:
E0CXC3E0CXC3_MOUSE
Fractalkine
Cx3cl1
37Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37D → G in AAB71763 (PubMed:9479488).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010586 mRNA Translation: AAB66331.1
U92565 mRNA Translation: AAB71763.1
AF071549 mRNA Translation: AAD41620.1
AK138214 mRNA Translation: BAE23585.1
AK138472 mRNA Translation: BAE23673.1
AK138574 mRNA Translation: BAE23702.1
AK154646 mRNA Translation: BAE32737.1
CH466525 Genomic DNA Translation: EDL11144.1
BC006650 mRNA Translation: AAH06650.1
BC054838 mRNA Translation: AAH54838.1
CCDSiCCDS22548.1
RefSeqiNP_033168.2, NM_009142.3
UniGeneiMm.103711

Genome annotation databases

EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778
GeneIDi20312
KEGGimmu:20312
UCSCiuc009mwx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010586 mRNA Translation: AAB66331.1
U92565 mRNA Translation: AAB71763.1
AF071549 mRNA Translation: AAD41620.1
AK138214 mRNA Translation: BAE23585.1
AK138472 mRNA Translation: BAE23673.1
AK138574 mRNA Translation: BAE23702.1
AK154646 mRNA Translation: BAE32737.1
CH466525 Genomic DNA Translation: EDL11144.1
BC006650 mRNA Translation: AAH06650.1
BC054838 mRNA Translation: AAH54838.1
CCDSiCCDS22548.1
RefSeqiNP_033168.2, NM_009142.3
UniGeneiMm.103711

3D structure databases

ProteinModelPortaliO35188
SMRiO35188
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35188, 1 interactor
MINTiO35188
STRINGi10090.ENSMUSP00000034230

PTM databases

iPTMnetiO35188
PhosphoSitePlusiO35188

Proteomic databases

MaxQBiO35188
PaxDbiO35188
PeptideAtlasiO35188
PRIDEiO35188

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034230; ENSMUSP00000034230; ENSMUSG00000031778
GeneIDi20312
KEGGimmu:20312
UCSCiuc009mwx.1 mouse

Organism-specific databases

CTDi6376
MGIiMGI:1097153 Cx3cl1

Phylogenomic databases

eggNOGiENOG410IJQZ Eukaryota
ENOG41116BY LUCA
GeneTreeiENSGT00510000049483
HOGENOMiHOG000036946
HOVERGENiHBG057269
InParanoidiO35188
KOiK05508
OMAiYQSLQGC
OrthoDBiEOG091G0WNR
TreeFamiTF337534

Enzyme and pathway databases

ReactomeiR-MMU-380108 Chemokine receptors bind chemokines
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:O35188
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031778 Expressed in 225 organ(s), highest expression level in prefrontal cortex
CleanExiMM_CX3CL1
ExpressionAtlasiO35188 baseline and differential
GenevisibleiO35188 MM

Family and domain databases

CDDicd00274 Chemokine_CX3C, 1 hit
InterProiView protein in InterPro
IPR039809 Chemokine_b/g/d
IPR034127 Chemokine_CX3C
IPR001811 Chemokine_IL8-like_dom
IPR008097 CX3CL1
IPR036048 Interleukin_8-like_sf
PANTHERiPTHR12015 PTHR12015, 1 hit
PTHR12015:SF92 PTHR12015:SF92, 1 hit
PfamiView protein in Pfam
PF00048 IL8, 1 hit
SMARTiView protein in SMART
SM00199 SCY, 1 hit
SUPFAMiSSF54117 SSF54117, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiX3CL1_MOUSE
AccessioniPrimary (citable) accession number: O35188
Secondary accession number(s): O35933, Q91V44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 149 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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