Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pituitary homeobox 3

Gene

Pitx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi62 – 121HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary homeobox 3
Alternative name(s):
Homeobox protein PITX3
Paired-like homeodomain transcription factor 3
Gene namesi
Name:Pitx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1100498 Pitx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Mutations in Pitx3 appear to be the cause of the aphakia (ak) phenotype, a recessive homozygous disease characterized by small eyes and closed eyelids.1 Publication

Disruption phenotypei

Mice show loss of nascent substantia nigra dopaminergic neurons at the beginning of their final differentiation and a loss of tyrosine hydroxylase (TH) expression specifically in the substantia nigra neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000492301 – 302Pituitary homeobox 3Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineBy similarity1
Modified residuei57PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35160
PRIDEiO35160

PTM databases

PhosphoSitePlusiO35160

Expressioni

Tissue specificityi

Highly expressed in developing eye lens. Expression is restricted to the substantia nigra and ventral tegmental area in the midbrain.2 Publications

Developmental stagei

First visible in E10.5 embryos where expression is confined to the lens vesicles. Between E11.5 and E12.5, expressed in both the lens epithelium and differentiating primary fiber cells. In the late fetal stage after the lens is formed, primarily found in the lens epithelium and the lens equator region where lens epithelial cells exit from the cell cycle and differentiate into fiber cells (at protein level). First expressed in the eye at 10 dpc embryos. Throughout eye development, expressed in the lens placode and forming lens pit. From 12 dpc, also detected in the midbrain region, tongue, incisor primordia, condensing mesenchyme around the sternum and vertebrae and in the head muscles.2 Publications

Gene expression databases

BgeeiENSMUSG00000025229 Expressed in 49 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_PITX3
ExpressionAtlasiO35160 baseline and differential
GenevisibleiO35160 MM

Interactioni

Subunit structurei

Interacts with SFPQ.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202188, 1 interactor
STRINGi10090.ENSMUSP00000026259

Structurei

3D structure databases

ProteinModelPortaliO35160
SMRiO35160
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 275OARPROSITE-ProRule annotationAdd BLAST14
Motifi268 – 272Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi240 – 250Poly-AlaAdd BLAST11

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0486 Eukaryota
ENOG410ZZ6J LUCA
GeneTreeiENSGT00930000150861
HOGENOMiHOG000273886
HOVERGENiHBG068770
InParanoidiO35160
KOiK09357
OMAiLCKGGFA
OrthoDBiEOG091G0H7U
PhylomeDBiO35160
TreeFamiTF351940

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR016233 Homeobox_Pitx/unc30
IPR003654 OAR_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF03826 OAR, 1 hit
PIRSFiPIRSF000563 Homeobox_protein_Pitx/Unc30, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS50803 OAR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35160-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEFGLLGEAE ARSPALSLSD AGTPHPPLPE HGCKGQEHSD SEKASASLPG
60 70 80 90 100
GSPEDGSLKK KQRRQRTHFT SQQLQELEAT FQRNRYPDMS TREEIAVWTN
110 120 130 140 150
LTEARVRVWF KNRRAKWRKR ERSQQAELCK GGFAAPLGGL VPPYEEVYPG
160 170 180 190 200
YSYGNWPPKA LAPPLAAKTF PFAFNSVNVG PLASQPVFSP PSSIAASMVP
210 220 230 240 250
SAAAAPGTVP GPGALQGLGG APPGLAPAAV SSGAVSCPYA SAAAAAAAAA
260 270 280 290 300
SSPYVYRDPC NSSLASLRLK AKQHASFSYP AVPGPPPAAN LSPCQYAVER

PV
Length:302
Mass (Da):31,715
Last modified:January 1, 1998 - v1
Checksum:iEB6EF6863B349264
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UZN3G3UZN3_MOUSE
Pituitary homeobox 3
Pitx3
90Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005772 mRNA Translation: AAB87380.1
BC120844 mRNA Translation: AAI20845.1
BC137810 mRNA Translation: AAI37811.1
CCDSiCCDS29872.1
RefSeqiNP_032878.1, NM_008852.4
UniGeneiMm.6255

Genome annotation databases

EnsembliENSMUST00000026259; ENSMUSP00000026259; ENSMUSG00000025229
GeneIDi18742
KEGGimmu:18742
UCSCiuc008hsl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005772 mRNA Translation: AAB87380.1
BC120844 mRNA Translation: AAI20845.1
BC137810 mRNA Translation: AAI37811.1
CCDSiCCDS29872.1
RefSeqiNP_032878.1, NM_008852.4
UniGeneiMm.6255

3D structure databases

ProteinModelPortaliO35160
SMRiO35160
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202188, 1 interactor
STRINGi10090.ENSMUSP00000026259

PTM databases

PhosphoSitePlusiO35160

Proteomic databases

PaxDbiO35160
PRIDEiO35160

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026259; ENSMUSP00000026259; ENSMUSG00000025229
GeneIDi18742
KEGGimmu:18742
UCSCiuc008hsl.1 mouse

Organism-specific databases

CTDi5309
MGIiMGI:1100498 Pitx3

Phylogenomic databases

eggNOGiKOG0486 Eukaryota
ENOG410ZZ6J LUCA
GeneTreeiENSGT00930000150861
HOGENOMiHOG000273886
HOVERGENiHBG068770
InParanoidiO35160
KOiK09357
OMAiLCKGGFA
OrthoDBiEOG091G0H7U
PhylomeDBiO35160
TreeFamiTF351940

Miscellaneous databases

PROiPR:O35160
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025229 Expressed in 49 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_PITX3
ExpressionAtlasiO35160 baseline and differential
GenevisibleiO35160 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR016233 Homeobox_Pitx/unc30
IPR003654 OAR_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF03826 OAR, 1 hit
PIRSFiPIRSF000563 Homeobox_protein_Pitx/Unc30, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS50803 OAR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPITX3_MOUSE
AccessioniPrimary (citable) accession number: O35160
Secondary accession number(s): Q0VB03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again