UniProtKB - O35160 (PITX3_MOUSE)
Pituitary homeobox 3
Pitx3
Functioni
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
5 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 62 – 121 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- anatomical structure morphogenesis Source: GO_Central
- cellular response to glial cell derived neurotrophic factor Source: Ensembl
- dopaminergic neuron differentiation Source: UniProtKB
- lens development in camera-type eye Source: UniProtKB
- lens fiber cell differentiation Source: UniProtKB
- lens morphogenesis in camera-type eye Source: UniProtKB
- locomotory behavior Source: MGI
- midbrain development Source: UniProtKB
- negative regulation of gliogenesis Source: MGI
- negative regulation of neurogenesis Source: MGI
- neuron development Source: MGI
- positive regulation of cell proliferation in midbrain Source: MGI
- positive regulation of neuron apoptotic process Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of gene expression Source: MGI
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to cocaine Source: Ensembl
- response to immobilization stress Source: Ensembl
- response to methamphetamine hydrochloride Source: Ensembl
- response to morphine Source: Ensembl
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Pituitary homeobox 3Alternative name(s): Homeobox protein PITX3 Paired-like homeodomain transcription factor 3 |
Gene namesi | Name:Pitx3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1100498, Pitx3 |
VEuPathDBi | HostDB:ENSMUSG00000025229 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049230 | 1 – 302 | Pituitary homeobox 3Add BLAST | 302 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 57 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O35160 |
PRIDEi | O35160 |
ProteomicsDBi | 289587 |
PTM databases
PhosphoSitePlusi | O35160 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000025229, Expressed in lens of camera-type eye and 82 other tissues |
ExpressionAtlasi | O35160, baseline and differential |
Genevisiblei | O35160, MM |
Interactioni
Subunit structurei
Interacts with SFPQ.
1 PublicationProtein-protein interaction databases
BioGRIDi | 202188, 1 interactor |
IntActi | O35160, 1 interactor |
STRINGi | 10090.ENSMUSP00000026259 |
Miscellaneous databases
RNActi | O35160, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 71 | DisorderedSequence analysisAdd BLAST | 71 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 262 – 275 | OARPROSITE-ProRule annotationAdd BLAST | 14 | |
Motifi | 268 – 272 | Nuclear localization signalSequence analysis | 5 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0486, Eukaryota |
GeneTreei | ENSGT00940000161801 |
HOGENOMi | CLU_030301_0_0_1 |
InParanoidi | O35160 |
OMAi | PLPEHSC |
OrthoDBi | 1432356at2759 |
PhylomeDBi | O35160 |
TreeFami | TF351940 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR016233, Homeobox_Pitx/unc30 IPR003654, OAR_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03826, OAR, 1 hit |
PIRSFi | PIRSF000563, Homeobox_protein_Pitx/Unc30, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS50803, OAR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEFGLLGEAE ARSPALSLSD AGTPHPPLPE HGCKGQEHSD SEKASASLPG
60 70 80 90 100
GSPEDGSLKK KQRRQRTHFT SQQLQELEAT FQRNRYPDMS TREEIAVWTN
110 120 130 140 150
LTEARVRVWF KNRRAKWRKR ERSQQAELCK GGFAAPLGGL VPPYEEVYPG
160 170 180 190 200
YSYGNWPPKA LAPPLAAKTF PFAFNSVNVG PLASQPVFSP PSSIAASMVP
210 220 230 240 250
SAAAAPGTVP GPGALQGLGG APPGLAPAAV SSGAVSCPYA SAAAAAAAAA
260 270 280 290 300
SSPYVYRDPC NSSLASLRLK AKQHASFSYP AVPGPPPAAN LSPCQYAVER
PV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3UZN3 | G3UZN3_MOUSE | Pituitary homeobox 3 | Pitx3 | 90 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF005772 mRNA Translation: AAB87380.1 BC120844 mRNA Translation: AAI20845.1 BC137810 mRNA Translation: AAI37811.1 |
CCDSi | CCDS29872.1 |
RefSeqi | NP_032878.1, NM_008852.4 |
Genome annotation databases
Ensembli | ENSMUST00000026259; ENSMUSP00000026259; ENSMUSG00000025229 |
GeneIDi | 18742 |
KEGGi | mmu:18742 |
UCSCi | uc008hsl.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF005772 mRNA Translation: AAB87380.1 BC120844 mRNA Translation: AAI20845.1 BC137810 mRNA Translation: AAI37811.1 |
CCDSi | CCDS29872.1 |
RefSeqi | NP_032878.1, NM_008852.4 |
3D structure databases
AlphaFoldDBi | O35160 |
BMRBi | O35160 |
SMRi | O35160 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202188, 1 interactor |
IntActi | O35160, 1 interactor |
STRINGi | 10090.ENSMUSP00000026259 |
PTM databases
PhosphoSitePlusi | O35160 |
Proteomic databases
PaxDbi | O35160 |
PRIDEi | O35160 |
ProteomicsDBi | 289587 |
Protocols and materials databases
Antibodypediai | 18010, 211 antibodies from 34 providers |
DNASUi | 18742 |
Genome annotation databases
Ensembli | ENSMUST00000026259; ENSMUSP00000026259; ENSMUSG00000025229 |
GeneIDi | 18742 |
KEGGi | mmu:18742 |
UCSCi | uc008hsl.1, mouse |
Organism-specific databases
CTDi | 5309 |
MGIi | MGI:1100498, Pitx3 |
VEuPathDBi | HostDB:ENSMUSG00000025229 |
Phylogenomic databases
eggNOGi | KOG0486, Eukaryota |
GeneTreei | ENSGT00940000161801 |
HOGENOMi | CLU_030301_0_0_1 |
InParanoidi | O35160 |
OMAi | PLPEHSC |
OrthoDBi | 1432356at2759 |
PhylomeDBi | O35160 |
TreeFami | TF351940 |
Miscellaneous databases
BioGRID-ORCSi | 18742, 0 hits in 75 CRISPR screens |
PROi | PR:O35160 |
RNActi | O35160, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025229, Expressed in lens of camera-type eye and 82 other tissues |
ExpressionAtlasi | O35160, baseline and differential |
Genevisiblei | O35160, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR016233, Homeobox_Pitx/unc30 IPR003654, OAR_dom |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03826, OAR, 1 hit |
PIRSFi | PIRSF000563, Homeobox_protein_Pitx/Unc30, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS50803, OAR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PITX3_MOUSE | |
Accessioni | O35160Primary (citable) accession number: O35160 Secondary accession number(s): Q0VB03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families