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Protein

ATPase inhibitor, mitochondrial

Gene

ATP5IF1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase inhibitor, mitochondrialCurated
Alternative name(s):
ATP synthase F1 subunit epsilonBy similarity
Inhibitor of F(1)F(o)-ATPase
Short name:
IF(1)
Short name:
IF1
Gene namesi
Name:ATP5IF1By similarity
Synonyms:Atpi, Atpif1, If1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196457 Atpif1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionBy similarityAdd BLAST25
ChainiPRO_000000254826 – 106ATPase inhibitor, mitochondrialAdd BLAST81

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineBy similarity1
Modified residuei103N6-succinyllysineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35143
PaxDbiO35143
PeptideAtlasiO35143
PRIDEiO35143
TopDownProteomicsiO35143

PTM databases

iPTMnetiO35143
PhosphoSitePlusiO35143

Expressioni

Gene expression databases

BgeeiENSMUSG00000054428 Expressed in 300 organ(s), highest expression level in striatum
CleanExiMM_ATPIF1
ExpressionAtlasiO35143 baseline and differential
GenevisibleiO35143 MM

Interactioni

Subunit structurei

Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198273, 1 interactor
IntActiO35143, 1 interactor
MINTiO35143
STRINGi10090.ENSMUSP00000064282

Structurei

3D structure databases

ProteinModelPortaliO35143
SMRiO35143
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 52N-terminal inhibitory regionBy similarityAdd BLAST27
Regioni74 – 106Antiparallel alpha-helical coiled coil regionBy similarityAdd BLAST33

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili60 – 106Sequence analysisAdd BLAST47

Domaini

Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 74-106, leaving each N-terminal inhibitory region (residues 26-52) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 26-52) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the ATPase inhibitor family.Curated

Keywords - Domaini

Coiled coil, Transit peptide

Phylogenomic databases

eggNOGiENOG410J2TJ Eukaryota
ENOG41127Z5 LUCA
GeneTreeiENSGT00390000006264
HOGENOMiHOG000247022
HOVERGENiHBG061381
InParanoidiO35143
KOiK22255
OrthoDBiEOG091G1B33
TreeFamiTF320659

Family and domain databases

InterProiView protein in InterPro
IPR007648 ATPase_inhibitor_mt
PfamiView protein in Pfam
PF04568 IATP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35143-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGSALAVRA RFGVWGMKVL QTRGFVSDSS DSMDTGAGSI REAGGAFGKR
60 70 80 90 100
EKAEEDRYFR EKTKEQLAAL RKHHEDEIDH HSKEIERLQK QIERHKKKIQ

QLKNNH
Length:106
Mass (Da):12,159
Last modified:October 3, 2012 - v2
Checksum:i78C0FE0CD04FA2D3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PV44E9PV44_MOUSE
ATPase inhibitory factor 1
Atpif1
74Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93E → D in AAB69647 (PubMed:9497501).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002718 mRNA Translation: AAB69647.1
AK008346 mRNA Translation: BAB25618.1
AK152389 mRNA Translation: BAE31177.1
AL805897 Genomic DNA Translation: CAM26517.1
CH466552 Genomic DNA Translation: EDL30101.1
BC012680 mRNA Translation: AAH12680.1
CCDSiCCDS18727.1
RefSeqiNP_031538.2, NM_007512.4
UniGeneiMm.2171

Genome annotation databases

EnsembliENSMUST00000067496; ENSMUSP00000064282; ENSMUSG00000054428
GeneIDi11983
KEGGimmu:11983
UCSCiuc008vbl.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002718 mRNA Translation: AAB69647.1
AK008346 mRNA Translation: BAB25618.1
AK152389 mRNA Translation: BAE31177.1
AL805897 Genomic DNA Translation: CAM26517.1
CH466552 Genomic DNA Translation: EDL30101.1
BC012680 mRNA Translation: AAH12680.1
CCDSiCCDS18727.1
RefSeqiNP_031538.2, NM_007512.4
UniGeneiMm.2171

3D structure databases

ProteinModelPortaliO35143
SMRiO35143
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198273, 1 interactor
IntActiO35143, 1 interactor
MINTiO35143
STRINGi10090.ENSMUSP00000064282

PTM databases

iPTMnetiO35143
PhosphoSitePlusiO35143

Proteomic databases

EPDiO35143
PaxDbiO35143
PeptideAtlasiO35143
PRIDEiO35143
TopDownProteomicsiO35143

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067496; ENSMUSP00000064282; ENSMUSG00000054428
GeneIDi11983
KEGGimmu:11983
UCSCiuc008vbl.3 mouse

Organism-specific databases

CTDi11983
MGIiMGI:1196457 Atpif1

Phylogenomic databases

eggNOGiENOG410J2TJ Eukaryota
ENOG41127Z5 LUCA
GeneTreeiENSGT00390000006264
HOGENOMiHOG000247022
HOVERGENiHBG061381
InParanoidiO35143
KOiK22255
OrthoDBiEOG091G1B33
TreeFamiTF320659

Miscellaneous databases

PROiPR:O35143
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054428 Expressed in 300 organ(s), highest expression level in striatum
CleanExiMM_ATPIF1
ExpressionAtlasiO35143 baseline and differential
GenevisibleiO35143 MM

Family and domain databases

InterProiView protein in InterPro
IPR007648 ATPase_inhibitor_mt
PfamiView protein in Pfam
PF04568 IATP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATIF1_MOUSE
AccessioniPrimary (citable) accession number: O35143
Secondary accession number(s): Q9D879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 3, 2012
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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