UniProtKB - O35137 (ALX4_MOUSE)
Protein
Homeobox protein aristaless-like 4
Gene
Alx4
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor involved in skull and limb development.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 202 – 261 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- HMG box domain binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- anterior/posterior pattern specification Source: MGI
- digestive tract development Source: MGI
- embryonic digit morphogenesis Source: MGI
- embryonic forelimb morphogenesis Source: MGI
- embryonic hindlimb morphogenesis Source: MGI
- embryonic skeletal system morphogenesis Source: MGI
- hair follicle development Source: MGI
- limb morphogenesis Source: MGI
- muscle organ development Source: MGI
- pattern specification process Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- post-embryonic development Source: MGI
- regulation of apoptotic process Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- roof of mouth development Source: MGI
- skeletal system morphogenesis Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein aristaless-like 4Alternative name(s): ALX-4 |
Gene namesi | Name:Alx4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108359, Alx4 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Defects in Alx4 are the cause of Strong luxoid (lst) phenotype. At heterozygosity lst is characterized by preaxial abnormalities of the hindfeet and, very rarely, of the forefeet. Homozygotes show preaxial polydactyly of all four limbs, reductions and duplications of the radius, absence of the tibia, craniofacial defects, reduction of the pubis, and dorsal alopecia.1 Publication
Keywords - Diseasei
Disease variantPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048815 | 1 – 399 | Homeobox protein aristaless-like 4Add BLAST | 399 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 188 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | O35137 |
PaxDbi | O35137 |
PRIDEi | O35137 |
ProteomicsDBi | 296398 |
PTM databases
iPTMneti | O35137 |
PhosphoSitePlusi | O35137 |
Expressioni
Tissue specificityi
Expressed in osteoblasts. Not expressed in brain, heart, intestine, kidney, liver, muscle, spleen and testis.
Developmental stagei
Expressed from 8.25 dpc and confined to mesenchymal cells throughout the embryo development. Expression is seen at several sites including craniofacial region, first branchial arch and anterior aspect of the limb bud.
Gene expression databases
Bgeei | ENSMUSG00000040310, Expressed in head and 99 other tissues |
ExpressionAtlasi | O35137, baseline and differential |
Genevisiblei | O35137, MM |
Interactioni
Subunit structurei
Binds DNA.
GO - Molecular functioni
- HMG box domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 198081, 32 interactors |
STRINGi | 10090.ENSMUSP00000047962 |
Miscellaneous databases
RNActi | O35137, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 379 – 392 | OARPROSITE-ProRule annotationAdd BLAST | 14 |
Sequence similaritiesi
Belongs to the paired homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000159662 |
HOGENOMi | CLU_047013_0_0_1 |
InParanoidi | O35137 |
OMAi | CDTVTPC |
OrthoDBi | 738339at2759 |
PhylomeDBi | O35137 |
TreeFami | TF350743 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR033203, ALX4 IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR003654, OAR_dom |
PANTHERi | PTHR24329:SF322, PTHR24329:SF322, 3 hits |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03826, OAR, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS50803, OAR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O35137-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNAETCVSYC ESPAAAMDAY YSPVSQSREG SSPFRGFPGG DKFGTTFLSA
60 70 80 90 100
GAKGQGFGDA KSRARYGAGQ QDLAAPLESS SGARGSFNKF QPQPPTPQPP
110 120 130 140 150
PAPPAPPAHL YLQRGACKTP PDGSLKLQEG SGGHNAALQV PCYAKESNLG
160 170 180 190 200
EPELPPDSEP VGMDNSYLSV KETGAKGPQD RASAEIPSPL EKTDSESNKG
210 220 230 240 250
KKRRNRTTFT SYQLEELEKV FQKTHYPDVY AREQLAMRTD LTEARVQVWF
260 270 280 290 300
QNRRAKWRKR ERFGQMQQVR THFSTAYELP LLTRAENYAQ IQNPSWIGNN
310 320 330 340 350
GAASPVPACV VPCDPVPACM SPHAHPPGSG ASSVSDFLSV SGAGSHVGQT
360 370 380 390
HMGSLFGAAG ISPGLNGYEM NGEPDRKTSS IAALRMKAKE HSAAISWAT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AIK5 | A2AIK5_MOUSE | Homeobox protein aristaless-like 4 | Alx4 | 325 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 206 | R → Q in lst; abolishes DNA binding and transcriptional activation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001465 mRNA Translation: AAC39943.1 |
CCDSi | CCDS16455.1 |
PIRi | JC6522 |
RefSeqi | NP_031468.1, NM_007442.3 |
Genome annotation databases
Ensembli | ENSMUST00000042078; ENSMUSP00000047962; ENSMUSG00000040310 |
GeneIDi | 11695 |
KEGGi | mmu:11695 |
UCSCi | uc012bzt.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001465 mRNA Translation: AAC39943.1 |
CCDSi | CCDS16455.1 |
PIRi | JC6522 |
RefSeqi | NP_031468.1, NM_007442.3 |
3D structure databases
BMRBi | O35137 |
SMRi | O35137 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198081, 32 interactors |
STRINGi | 10090.ENSMUSP00000047962 |
PTM databases
iPTMneti | O35137 |
PhosphoSitePlusi | O35137 |
Proteomic databases
MaxQBi | O35137 |
PaxDbi | O35137 |
PRIDEi | O35137 |
ProteomicsDBi | 296398 |
Protocols and materials databases
Antibodypediai | 13210, 293 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042078; ENSMUSP00000047962; ENSMUSG00000040310 |
GeneIDi | 11695 |
KEGGi | mmu:11695 |
UCSCi | uc012bzt.1, mouse |
Organism-specific databases
CTDi | 60529 |
MGIi | MGI:108359, Alx4 |
Phylogenomic databases
eggNOGi | KOG0490, Eukaryota |
GeneTreei | ENSGT00940000159662 |
HOGENOMi | CLU_047013_0_0_1 |
InParanoidi | O35137 |
OMAi | CDTVTPC |
OrthoDBi | 738339at2759 |
PhylomeDBi | O35137 |
TreeFami | TF350743 |
Miscellaneous databases
BioGRID-ORCSi | 11695, 0 hits in 52 CRISPR screens |
PROi | PR:O35137 |
RNActi | O35137, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040310, Expressed in head and 99 other tissues |
ExpressionAtlasi | O35137, baseline and differential |
Genevisiblei | O35137, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR033203, ALX4 IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR003654, OAR_dom |
PANTHERi | PTHR24329:SF322, PTHR24329:SF322, 3 hits |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03826, OAR, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS50803, OAR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALX4_MOUSE | |
Accessioni | O35137Primary (citable) accession number: O35137 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families