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UniProtKB - O35134 (RPA1_MOUSE)

Protein

DNA-directed RNA polymerase I subunit RPA1

Gene

Polr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64ZincBy similarity1
Metal bindingi67ZincBy similarity1
Metal bindingi74ZincBy similarity1
Metal bindingi77ZincBy similarity1
Metal bindingi595Magnesium; catalyticBy similarity1
Metal bindingi597Magnesium; catalyticBy similarity1
Metal bindingi599Magnesium; catalyticBy similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA1 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit A1
Alternative name(s):
DNA-directed RNA polymerase I largest subunit
DNA-directed RNA polymerase I subunit A
RNA polymerase I 194 kDa subunit
Short name:
RPA194
Gene namesi
Name:Polr1a
Synonyms:Rpa1, Rpo1-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1096397 Polr1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739241 – 1717DNA-directed RNA polymerase I subunit RPA1Add BLAST1717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1393PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35134
MaxQBiO35134
PaxDbiO35134
PeptideAtlasiO35134
PRIDEiO35134

PTM databases

iPTMnetiO35134
PhosphoSitePlusiO35134

Expressioni

Gene expression databases

BgeeiENSMUSG00000049553 Expressed in 275 organ(s), highest expression level in embryonic stem cell
CleanExiMM_RPA1
ExpressionAtlasiO35134 baseline and differential
GenevisibleiO35134 MM

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity). Interacts with MYO1C (PubMed:16514417). Interacts with ERBB2 (By similarity). Interacts with DDX11 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi202995, 8 interactors
STRINGi10090.ENSMUSP00000060858

Structurei

3D structure databases

ProteinModelPortaliO35134
SMRiO35134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni968 – 980Bridging helixBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0262 Eukaryota
COG0086 LUCA
GeneTreeiENSGT00920000149138
HOGENOMiHOG000205401
HOVERGENiHBG017741
InParanoidiO35134
KOiK02999
OMAiRFFNRED
OrthoDBiEOG091G00IM
PhylomeDBiO35134
TreeFamiTF103033

Family and domain databases

Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR015699 DNA-dir_RNA_pol1_lsu
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR038120 Rpb1_funnel_sf
PANTHERiPTHR19376:SF11 PTHR19376:SF11, 1 hit
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O35134-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLASKHTPWR RLQGISFGMY SAEELKKLSV KSITNPRYVD YLGNPSANGL 
        60         70         80         90        100
YDLALGPADS KEVCATCVQD FNNCSGHLGH IDLPLTVYNP FLFDKLYLLL 
       110        120        130        140        150
RGSCLSCHML TCPRAAIYLL ISQLRVLEVG ALQAVYELER ILSRFLEETG 
       160        170        180        190        200
DPSAFEIQEE LEEYTSKILQ NNLLGSQGTH VKNVCESRSK LVAQFWKTHM 
       210        220        230        240        250
AAKQCPHCKT GRSVVRKEHN SKLIITYPAT VHKKSDQEGT ELPEGVPEAP 
       260        270        280        290        300
GIDKAQMGKR GYLTPSSAQE HLFAIWKNEG FFLNYLFSGL DDIGPESSFN 
       310        320        330        340        350
PSMFFLDFIV VPPSRYRPVN RLGDQMFTNG QTVNLQAVMK DAVLIRKLLA 
       360        370        380        390        400
LMAQEQKLPC EMTELTIDKE NDSSVAIDRS FLGLLPGPSL TDKLYNIWIR 
       410        420        430        440        450
LQSHVNIVFD SEMDKLMLEK YPGIRQILEK KEGLFRKHMM GKRVDYAARS 
       460        470        480        490        500
VICPDMYINT NEIGIPMVFA TKLTYPQPVT PWNVQELRQA VINGPNVHPG 
       510        520        530        540        550
ASMVINEDGS RTALSSVDAA QREAVAKQLL TPATGAPKPQ GTKVVCRHVK 
       560        570        580        590        600
NGDILLLNRQ PTLHRPSIQA HRARILPEEK VLRLHYANCK AYNADFDGDE 
       610        620        630        640        650
MNAHFPQSEL GRAEAYVLAC TDQQYLVPKD GQPLAGLIQD HMVSGANMTI 
       660        670        680        690        700
RGCFFTREQY MELVYRGLTD KVGRVKLFPP AILKPFPLWT GKQVVSTLLI 
       710        720        730        740        750
NIIPEDYAPL NLSGKAKIGS KAWVKEKPRP IPDFDPDSMC ESQVIIREGE 
       760        770        780        790        800
LLCGVLDKAH YGSSAYGLVH CCYEIYGGET SGRVLTCLAR LFTAYLQLYR 
       810        820        830        840        850
GFTLGVEDIL VKPNADVVRQ RIIEESTQCG PQAVKAALSL PETASCDEIQ 
       860        870        880        890        900
GKWQDAHLSK DQRDFNMIDM KFKEEVNHYS NEINKACMPL GLHRQFPENN 
       910        920        930        940        950
LQMMVQSGAK GSTVNTMQIS CLLGQIELEG RRPPLMASGK SLPCFEPYEF 
       960        970        980        990       1000
TPRAGGFVTG RFLTGIRPPE FFFHCMAGRE GLVDTAVKTS RSGYLQRCII 
      1010       1020       1030       1040       1050
KHLEGLVIQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ PKQFPFLAGN 
      1060       1070       1080       1090       1100
YEVIMKSKHL HEVLSRADPQ KVLGHIKAIK KWHHKHSGAL LRKGAFLSFS 
      1110       1120       1130       1140       1150
QKIQAAVKAL NLKGSIQNGR SPETQQMLQM WYDLDEESRW KYQKRAAPCP 
      1160       1170       1180       1190       1200
DPSLSVWRPD IYFASVSETF EKKIDDFSQE WAAQAERSYK KSELSLDRLR 
      1210       1220       1230       1240       1250
TLLQLKWQRS LCDPGEAVGL LAAQSIGEPS TQMTLNTFHF AGRGEMNVTL 
      1260       1270       1280       1290       1300
GIPRLREILM VASANIKTPM MSVPVFDTKK ALKKVKSLKK RLTRVCLGEV 
      1310       1320       1330       1340       1350
LQKVDIQESF CMGEKRNKFQ VYELRFQFLP HAYYQQEKCL RPEDILHFME 
      1360       1370       1380       1390       1400
TRFFKLLMEA IKKKKNKASA FRNVNSRRAT QKDLNDTEDS GRSQREEERD 
      1410       1420       1430       1440       1450
EEEEGNIVDA EAEEGDADAS DTKRKEKQEE EVDYESEEEG EEEEEEEVQE 
      1460       1470       1480       1490       1500
EGNIKGDGVH QGHEPDEEEH LGLEEEESSQ KPPRRHSRPQ GAEAIKRRIQ 
      1510       1520       1530       1540       1550
AVRESYSFIE DYQYDTEESL WCQVTVKLPL MKINFDMSSL VVSLAHKAIV 
      1560       1570       1580       1590       1600
YTTKGITRCL LNETTNSKNE KELVLNTEGI NLPELFKYSE ILDLRRLYSN 
      1610       1620       1630       1640       1650
DIHAMANTYG IEAALRVIEK EIKDVFAVYG IAVDPRHLSL VADYMCFEGV 
      1660       1670       1680       1690       1700
YKPLNRFGIQ SSSSPLQQMT FETSFQFLKQ ATMMGSHDEL KSPSACLVVG 
      1710 
KVVKGGTGLF ELKQPLR
Length:1,717
Mass (Da):194,110
Last modified:December 7, 2004 - v2
Checksum:iE78B7E3F6F7129F1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPM9A0A0U1RPM9_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
743Annotation score:
A0A0U1RPX8A0A0U1RPX8_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
282Annotation score:
A0A0U1RNG3A0A0U1RNG3_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
48Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti821R → H in AAH53744 (PubMed:15489334).Curated1
Sequence conflicti1246M → T in AAB66718 (PubMed:9236775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA Translation: AAB66718.1
BC053744 mRNA Translation: AAH53744.1
CCDSiCCDS20236.1
PIRiT13961
RefSeqiNP_033114.3, NM_009088.3
UniGeneiMm.135581

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553
GeneIDi20019
KEGGimmu:20019
UCSCiuc009chv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA Translation: AAB66718.1
BC053744 mRNA Translation: AAH53744.1
CCDSiCCDS20236.1
PIRiT13961
RefSeqiNP_033114.3, NM_009088.3
UniGeneiMm.135581

3D structure databases

ProteinModelPortaliO35134
SMRiO35134
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202995, 8 interactors
STRINGi10090.ENSMUSP00000060858

PTM databases

iPTMnetiO35134
PhosphoSitePlusiO35134

Proteomic databases

EPDiO35134
MaxQBiO35134
PaxDbiO35134
PeptideAtlasiO35134
PRIDEiO35134

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553
GeneIDi20019
KEGGimmu:20019
UCSCiuc009chv.2 mouse

Organism-specific databases

CTDi25885
MGIiMGI:1096397 Polr1a

Phylogenomic databases

eggNOGiKOG0262 Eukaryota
COG0086 LUCA
GeneTreeiENSGT00920000149138
HOGENOMiHOG000205401
HOVERGENiHBG017741
InParanoidiO35134
KOiK02999
OMAiRFFNRED
OrthoDBiEOG091G00IM
PhylomeDBiO35134
TreeFamiTF103033

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Miscellaneous databases

ChiTaRSiPolr1a mouse
PROiPR:O35134
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049553 Expressed in 275 organ(s), highest expression level in embryonic stem cell
CleanExiMM_RPA1
ExpressionAtlasiO35134 baseline and differential
GenevisibleiO35134 MM

Family and domain databases

Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR015699 DNA-dir_RNA_pol1_lsu
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR038120 Rpb1_funnel_sf
PANTHERiPTHR19376:SF11 PTHR19376:SF11, 1 hit
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPA1_MOUSE
AccessioniPrimary (citable) accession number: O35134
Secondary accession number(s): Q7TSA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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