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Protein

DNA-directed RNA polymerase I subunit RPA1

Gene

Polr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64ZincBy similarity1
Metal bindingi67ZincBy similarity1
Metal bindingi74ZincBy similarity1
Metal bindingi77ZincBy similarity1
Metal bindingi595Magnesium; catalyticBy similarity1
Metal bindingi597Magnesium; catalyticBy similarity1
Metal bindingi599Magnesium; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA1 (EC:2.7.7.6)
Short name:
RNA polymerase I subunit A1
Alternative name(s):
DNA-directed RNA polymerase I largest subunit
DNA-directed RNA polymerase I subunit A
RNA polymerase I 194 kDa subunit
Short name:
RPA194
Gene namesi
Name:Polr1a
Synonyms:Rpa1, Rpo1-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1096397 Polr1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739241 – 1717DNA-directed RNA polymerase I subunit RPA1Add BLAST1717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1393PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35134
MaxQBiO35134
PaxDbiO35134
PeptideAtlasiO35134
PRIDEiO35134

PTM databases

iPTMnetiO35134
PhosphoSitePlusiO35134

Expressioni

Gene expression databases

BgeeiENSMUSG00000049553 Expressed in 275 organ(s), highest expression level in embryonic stem cell
CleanExiMM_RPA1
ExpressionAtlasiO35134 baseline and differential
GenevisibleiO35134 MM

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity). Interacts with MYO1C (PubMed:16514417). Interacts with ERBB2 (By similarity). Interacts with DDX11 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi202995, 8 interactors
STRINGi10090.ENSMUSP00000060858

Structurei

3D structure databases

ProteinModelPortaliO35134
SMRiO35134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni968 – 980Bridging helixBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

eggNOGiKOG0262 Eukaryota
COG0086 LUCA
GeneTreeiENSGT00920000149138
HOGENOMiHOG000205401
HOVERGENiHBG017741
InParanoidiO35134
KOiK02999
OMAiRFFNRED
OrthoDBiEOG091G00IM
PhylomeDBiO35134
TreeFamiTF103033

Family and domain databases

Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR015699 DNA-dir_RNA_pol1_lsu
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR038120 Rpb1_funnel_sf
PANTHERiPTHR19376:SF11 PTHR19376:SF11, 1 hit
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O35134-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLASKHTPWR RLQGISFGMY SAEELKKLSV KSITNPRYVD YLGNPSANGL
60 70 80 90 100
YDLALGPADS KEVCATCVQD FNNCSGHLGH IDLPLTVYNP FLFDKLYLLL
110 120 130 140 150
RGSCLSCHML TCPRAAIYLL ISQLRVLEVG ALQAVYELER ILSRFLEETG
160 170 180 190 200
DPSAFEIQEE LEEYTSKILQ NNLLGSQGTH VKNVCESRSK LVAQFWKTHM
210 220 230 240 250
AAKQCPHCKT GRSVVRKEHN SKLIITYPAT VHKKSDQEGT ELPEGVPEAP
260 270 280 290 300
GIDKAQMGKR GYLTPSSAQE HLFAIWKNEG FFLNYLFSGL DDIGPESSFN
310 320 330 340 350
PSMFFLDFIV VPPSRYRPVN RLGDQMFTNG QTVNLQAVMK DAVLIRKLLA
360 370 380 390 400
LMAQEQKLPC EMTELTIDKE NDSSVAIDRS FLGLLPGPSL TDKLYNIWIR
410 420 430 440 450
LQSHVNIVFD SEMDKLMLEK YPGIRQILEK KEGLFRKHMM GKRVDYAARS
460 470 480 490 500
VICPDMYINT NEIGIPMVFA TKLTYPQPVT PWNVQELRQA VINGPNVHPG
510 520 530 540 550
ASMVINEDGS RTALSSVDAA QREAVAKQLL TPATGAPKPQ GTKVVCRHVK
560 570 580 590 600
NGDILLLNRQ PTLHRPSIQA HRARILPEEK VLRLHYANCK AYNADFDGDE
610 620 630 640 650
MNAHFPQSEL GRAEAYVLAC TDQQYLVPKD GQPLAGLIQD HMVSGANMTI
660 670 680 690 700
RGCFFTREQY MELVYRGLTD KVGRVKLFPP AILKPFPLWT GKQVVSTLLI
710 720 730 740 750
NIIPEDYAPL NLSGKAKIGS KAWVKEKPRP IPDFDPDSMC ESQVIIREGE
760 770 780 790 800
LLCGVLDKAH YGSSAYGLVH CCYEIYGGET SGRVLTCLAR LFTAYLQLYR
810 820 830 840 850
GFTLGVEDIL VKPNADVVRQ RIIEESTQCG PQAVKAALSL PETASCDEIQ
860 870 880 890 900
GKWQDAHLSK DQRDFNMIDM KFKEEVNHYS NEINKACMPL GLHRQFPENN
910 920 930 940 950
LQMMVQSGAK GSTVNTMQIS CLLGQIELEG RRPPLMASGK SLPCFEPYEF
960 970 980 990 1000
TPRAGGFVTG RFLTGIRPPE FFFHCMAGRE GLVDTAVKTS RSGYLQRCII
1010 1020 1030 1040 1050
KHLEGLVIQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ PKQFPFLAGN
1060 1070 1080 1090 1100
YEVIMKSKHL HEVLSRADPQ KVLGHIKAIK KWHHKHSGAL LRKGAFLSFS
1110 1120 1130 1140 1150
QKIQAAVKAL NLKGSIQNGR SPETQQMLQM WYDLDEESRW KYQKRAAPCP
1160 1170 1180 1190 1200
DPSLSVWRPD IYFASVSETF EKKIDDFSQE WAAQAERSYK KSELSLDRLR
1210 1220 1230 1240 1250
TLLQLKWQRS LCDPGEAVGL LAAQSIGEPS TQMTLNTFHF AGRGEMNVTL
1260 1270 1280 1290 1300
GIPRLREILM VASANIKTPM MSVPVFDTKK ALKKVKSLKK RLTRVCLGEV
1310 1320 1330 1340 1350
LQKVDIQESF CMGEKRNKFQ VYELRFQFLP HAYYQQEKCL RPEDILHFME
1360 1370 1380 1390 1400
TRFFKLLMEA IKKKKNKASA FRNVNSRRAT QKDLNDTEDS GRSQREEERD
1410 1420 1430 1440 1450
EEEEGNIVDA EAEEGDADAS DTKRKEKQEE EVDYESEEEG EEEEEEEVQE
1460 1470 1480 1490 1500
EGNIKGDGVH QGHEPDEEEH LGLEEEESSQ KPPRRHSRPQ GAEAIKRRIQ
1510 1520 1530 1540 1550
AVRESYSFIE DYQYDTEESL WCQVTVKLPL MKINFDMSSL VVSLAHKAIV
1560 1570 1580 1590 1600
YTTKGITRCL LNETTNSKNE KELVLNTEGI NLPELFKYSE ILDLRRLYSN
1610 1620 1630 1640 1650
DIHAMANTYG IEAALRVIEK EIKDVFAVYG IAVDPRHLSL VADYMCFEGV
1660 1670 1680 1690 1700
YKPLNRFGIQ SSSSPLQQMT FETSFQFLKQ ATMMGSHDEL KSPSACLVVG
1710
KVVKGGTGLF ELKQPLR
Length:1,717
Mass (Da):194,110
Last modified:December 7, 2004 - v2
Checksum:iE78B7E3F6F7129F1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPM9A0A0U1RPM9_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
743Annotation score:
A0A0U1RPX8A0A0U1RPX8_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
282Annotation score:
A0A0U1RNG3A0A0U1RNG3_MOUSE
DNA-directed RNA polymerase I subun...
Polr1a
48Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti821R → H in AAH53744 (PubMed:15489334).Curated1
Sequence conflicti1246M → T in AAB66718 (PubMed:9236775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA Translation: AAB66718.1
BC053744 mRNA Translation: AAH53744.1
CCDSiCCDS20236.1
PIRiT13961
RefSeqiNP_033114.3, NM_009088.3
UniGeneiMm.135581

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553
GeneIDi20019
KEGGimmu:20019
UCSCiuc009chv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000938 mRNA Translation: AAB66718.1
BC053744 mRNA Translation: AAH53744.1
CCDSiCCDS20236.1
PIRiT13961
RefSeqiNP_033114.3, NM_009088.3
UniGeneiMm.135581

3D structure databases

ProteinModelPortaliO35134
SMRiO35134
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202995, 8 interactors
STRINGi10090.ENSMUSP00000060858

PTM databases

iPTMnetiO35134
PhosphoSitePlusiO35134

Proteomic databases

EPDiO35134
MaxQBiO35134
PaxDbiO35134
PeptideAtlasiO35134
PRIDEiO35134

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553
GeneIDi20019
KEGGimmu:20019
UCSCiuc009chv.2 mouse

Organism-specific databases

CTDi25885
MGIiMGI:1096397 Polr1a

Phylogenomic databases

eggNOGiKOG0262 Eukaryota
COG0086 LUCA
GeneTreeiENSGT00920000149138
HOGENOMiHOG000205401
HOVERGENiHBG017741
InParanoidiO35134
KOiK02999
OMAiRFFNRED
OrthoDBiEOG091G00IM
PhylomeDBiO35134
TreeFamiTF103033

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Miscellaneous databases

ChiTaRSiPolr1a mouse
PROiPR:O35134
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049553 Expressed in 275 organ(s), highest expression level in embryonic stem cell
CleanExiMM_RPA1
ExpressionAtlasiO35134 baseline and differential
GenevisibleiO35134 MM

Family and domain databases

Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR015699 DNA-dir_RNA_pol1_lsu
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR038120 Rpb1_funnel_sf
PANTHERiPTHR19376:SF11 PTHR19376:SF11, 1 hit
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPA1_MOUSE
AccessioniPrimary (citable) accession number: O35134
Secondary accession number(s): Q7TSA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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