UniProtKB - O35134 (RPA1_MOUSE)
Protein
DNA-directed RNA polymerase I subunit RPA1
Gene
Polr1a
Organism
Mus musculus (Mouse)
Status
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity
Catalytic activityi
- EC:2.7.7.6
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | ZincBy similarity | 1 | |
Metal bindingi | 67 | ZincBy similarity | 1 | |
Metal bindingi | 74 | ZincBy similarity | 1 | |
Metal bindingi | 77 | ZincBy similarity | 1 | |
Metal bindingi | 595 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 597 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 599 | Magnesium; catalyticBy similarity | 1 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- DNA binding Source: InterPro
- RNA polymerase I activity Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- negative regulation of protein localization to nucleolus Source: MGI
- rRNA transcription Source: MGI
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Transcription |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5250924, B-WICH complex positively regulates rRNA expression R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-73772, RNA Polymerase I Promoter Escape R-MMU-73863, RNA Polymerase I Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase I subunit RPA1 (EC:2.7.7.6)Short name: RNA polymerase I subunit A1 Alternative name(s): DNA-directed RNA polymerase I largest subunit DNA-directed RNA polymerase I subunit A RNA polymerase I 194 kDa subunit Short name: RPA194 |
Gene namesi | Name:Polr1a Synonyms:Rpa1, Rpo1-4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096397, Polr1a |
Subcellular locationi
Nucleus
- nucleolus By similarity
Other locations
- Chromosome 2 Publications
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
- RNA polymerase I complex Source: MGI
Keywords - Cellular componenti
Chromosome, DNA-directed RNA polymerase, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073924 | 1 – 1717 | DNA-directed RNA polymerase I subunit RPA1Add BLAST | 1717 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1393 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O35134 |
MaxQBi | O35134 |
PaxDbi | O35134 |
PeptideAtlasi | O35134 |
PRIDEi | O35134 |
PTM databases
iPTMneti | O35134 |
PhosphoSitePlusi | O35134 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000049553, Expressed in epiblast (generic) and 290 other tissues |
ExpressionAtlasi | O35134, baseline and differential |
Genevisiblei | O35134, MM |
Interactioni
Subunit structurei
Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity).
Interacts with MYO1C (PubMed:16514417).
Interacts with ERBB2 (By similarity).
Interacts with DDX11 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 202995, 27 interactors |
STRINGi | 10090.ENSMUSP00000060858 |
Miscellaneous databases
RNActi | O35134, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 968 – 980 | Bridging helixBy similarityAdd BLAST | 13 |
Sequence similaritiesi
Belongs to the RNA polymerase beta' chain family.Curated
Phylogenomic databases
eggNOGi | KOG0262, Eukaryota |
GeneTreei | ENSGT00920000149138 |
HOGENOMi | CLU_000487_2_4_1 |
InParanoidi | O35134 |
OMAi | RFFNRED |
OrthoDBi | 591636at2759 |
PhylomeDBi | O35134 |
TreeFami | TF103033 |
Family and domain databases
Gene3Di | 1.10.132.30, 1 hit 1.10.274.100, 1 hit |
InterProi | View protein in InterPro IPR015699, DNA-dir_RNA_pol1_lsu IPR000722, RNA_pol_asu IPR006592, RNA_pol_N IPR007080, RNA_pol_Rpb1_1 IPR007066, RNA_pol_Rpb1_3 IPR042102, RNA_pol_Rpb1_3_sf IPR007083, RNA_pol_Rpb1_4 IPR007081, RNA_pol_Rpb1_5 IPR038120, Rpb1_funnel_sf |
PANTHERi | PTHR19376:SF11, PTHR19376:SF11, 1 hit |
Pfami | View protein in Pfam PF04997, RNA_pol_Rpb1_1, 1 hit PF00623, RNA_pol_Rpb1_2, 1 hit PF04983, RNA_pol_Rpb1_3, 1 hit PF05000, RNA_pol_Rpb1_4, 1 hit PF04998, RNA_pol_Rpb1_5, 1 hit |
SMARTi | View protein in SMART SM00663, RPOLA_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O35134-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLASKHTPWR RLQGISFGMY SAEELKKLSV KSITNPRYVD YLGNPSANGL
60 70 80 90 100
YDLALGPADS KEVCATCVQD FNNCSGHLGH IDLPLTVYNP FLFDKLYLLL
110 120 130 140 150
RGSCLSCHML TCPRAAIYLL ISQLRVLEVG ALQAVYELER ILSRFLEETG
160 170 180 190 200
DPSAFEIQEE LEEYTSKILQ NNLLGSQGTH VKNVCESRSK LVAQFWKTHM
210 220 230 240 250
AAKQCPHCKT GRSVVRKEHN SKLIITYPAT VHKKSDQEGT ELPEGVPEAP
260 270 280 290 300
GIDKAQMGKR GYLTPSSAQE HLFAIWKNEG FFLNYLFSGL DDIGPESSFN
310 320 330 340 350
PSMFFLDFIV VPPSRYRPVN RLGDQMFTNG QTVNLQAVMK DAVLIRKLLA
360 370 380 390 400
LMAQEQKLPC EMTELTIDKE NDSSVAIDRS FLGLLPGPSL TDKLYNIWIR
410 420 430 440 450
LQSHVNIVFD SEMDKLMLEK YPGIRQILEK KEGLFRKHMM GKRVDYAARS
460 470 480 490 500
VICPDMYINT NEIGIPMVFA TKLTYPQPVT PWNVQELRQA VINGPNVHPG
510 520 530 540 550
ASMVINEDGS RTALSSVDAA QREAVAKQLL TPATGAPKPQ GTKVVCRHVK
560 570 580 590 600
NGDILLLNRQ PTLHRPSIQA HRARILPEEK VLRLHYANCK AYNADFDGDE
610 620 630 640 650
MNAHFPQSEL GRAEAYVLAC TDQQYLVPKD GQPLAGLIQD HMVSGANMTI
660 670 680 690 700
RGCFFTREQY MELVYRGLTD KVGRVKLFPP AILKPFPLWT GKQVVSTLLI
710 720 730 740 750
NIIPEDYAPL NLSGKAKIGS KAWVKEKPRP IPDFDPDSMC ESQVIIREGE
760 770 780 790 800
LLCGVLDKAH YGSSAYGLVH CCYEIYGGET SGRVLTCLAR LFTAYLQLYR
810 820 830 840 850
GFTLGVEDIL VKPNADVVRQ RIIEESTQCG PQAVKAALSL PETASCDEIQ
860 870 880 890 900
GKWQDAHLSK DQRDFNMIDM KFKEEVNHYS NEINKACMPL GLHRQFPENN
910 920 930 940 950
LQMMVQSGAK GSTVNTMQIS CLLGQIELEG RRPPLMASGK SLPCFEPYEF
960 970 980 990 1000
TPRAGGFVTG RFLTGIRPPE FFFHCMAGRE GLVDTAVKTS RSGYLQRCII
1010 1020 1030 1040 1050
KHLEGLVIQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ PKQFPFLAGN
1060 1070 1080 1090 1100
YEVIMKSKHL HEVLSRADPQ KVLGHIKAIK KWHHKHSGAL LRKGAFLSFS
1110 1120 1130 1140 1150
QKIQAAVKAL NLKGSIQNGR SPETQQMLQM WYDLDEESRW KYQKRAAPCP
1160 1170 1180 1190 1200
DPSLSVWRPD IYFASVSETF EKKIDDFSQE WAAQAERSYK KSELSLDRLR
1210 1220 1230 1240 1250
TLLQLKWQRS LCDPGEAVGL LAAQSIGEPS TQMTLNTFHF AGRGEMNVTL
1260 1270 1280 1290 1300
GIPRLREILM VASANIKTPM MSVPVFDTKK ALKKVKSLKK RLTRVCLGEV
1310 1320 1330 1340 1350
LQKVDIQESF CMGEKRNKFQ VYELRFQFLP HAYYQQEKCL RPEDILHFME
1360 1370 1380 1390 1400
TRFFKLLMEA IKKKKNKASA FRNVNSRRAT QKDLNDTEDS GRSQREEERD
1410 1420 1430 1440 1450
EEEEGNIVDA EAEEGDADAS DTKRKEKQEE EVDYESEEEG EEEEEEEVQE
1460 1470 1480 1490 1500
EGNIKGDGVH QGHEPDEEEH LGLEEEESSQ KPPRRHSRPQ GAEAIKRRIQ
1510 1520 1530 1540 1550
AVRESYSFIE DYQYDTEESL WCQVTVKLPL MKINFDMSSL VVSLAHKAIV
1560 1570 1580 1590 1600
YTTKGITRCL LNETTNSKNE KELVLNTEGI NLPELFKYSE ILDLRRLYSN
1610 1620 1630 1640 1650
DIHAMANTYG IEAALRVIEK EIKDVFAVYG IAVDPRHLSL VADYMCFEGV
1660 1670 1680 1690 1700
YKPLNRFGIQ SSSSPLQQMT FETSFQFLKQ ATMMGSHDEL KSPSACLVVG
1710
KVVKGGTGLF ELKQPLR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RPM9 | A0A0U1RPM9_MOUSE | DNA-directed RNA polymerase | Polr1a | 743 | Annotation score: | ||
A0A0U1RPX8 | A0A0U1RPX8_MOUSE | DNA-directed RNA polymerase | Polr1a | 282 | Annotation score: | ||
A0A0U1RNG3 | A0A0U1RNG3_MOUSE | DNA-directed RNA polymerase I subun... | Polr1a | 48 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 821 | R → H in AAH53744 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1246 | M → T in AAB66718 (PubMed:9236775).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF000938 mRNA Translation: AAB66718.1 BC053744 mRNA Translation: AAH53744.1 |
CCDSi | CCDS20236.1 |
PIRi | T13961 |
RefSeqi | NP_033114.3, NM_009088.3 |
Genome annotation databases
Ensembli | ENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553 |
GeneIDi | 20019 |
KEGGi | mmu:20019 |
UCSCi | uc009chv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF000938 mRNA Translation: AAB66718.1 BC053744 mRNA Translation: AAH53744.1 |
CCDSi | CCDS20236.1 |
PIRi | T13961 |
RefSeqi | NP_033114.3, NM_009088.3 |
3D structure databases
SMRi | O35134 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202995, 27 interactors |
STRINGi | 10090.ENSMUSP00000060858 |
PTM databases
iPTMneti | O35134 |
PhosphoSitePlusi | O35134 |
Proteomic databases
EPDi | O35134 |
MaxQBi | O35134 |
PaxDbi | O35134 |
PeptideAtlasi | O35134 |
PRIDEi | O35134 |
Protocols and materials databases
Antibodypediai | 4117, 111 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000055296; ENSMUSP00000060858; ENSMUSG00000049553 |
GeneIDi | 20019 |
KEGGi | mmu:20019 |
UCSCi | uc009chv.2, mouse |
Organism-specific databases
CTDi | 25885 |
MGIi | MGI:1096397, Polr1a |
Phylogenomic databases
eggNOGi | KOG0262, Eukaryota |
GeneTreei | ENSGT00920000149138 |
HOGENOMi | CLU_000487_2_4_1 |
InParanoidi | O35134 |
OMAi | RFFNRED |
OrthoDBi | 591636at2759 |
PhylomeDBi | O35134 |
TreeFami | TF103033 |
Enzyme and pathway databases
Reactomei | R-MMU-5250924, B-WICH complex positively regulates rRNA expression R-MMU-73762, RNA Polymerase I Transcription Initiation R-MMU-73772, RNA Polymerase I Promoter Escape R-MMU-73863, RNA Polymerase I Transcription Termination |
Miscellaneous databases
BioGRID-ORCSi | 20019, 8 hits in 18 CRISPR screens |
ChiTaRSi | Polr1a, mouse |
PROi | PR:O35134 |
RNActi | O35134, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000049553, Expressed in epiblast (generic) and 290 other tissues |
ExpressionAtlasi | O35134, baseline and differential |
Genevisiblei | O35134, MM |
Family and domain databases
Gene3Di | 1.10.132.30, 1 hit 1.10.274.100, 1 hit |
InterProi | View protein in InterPro IPR015699, DNA-dir_RNA_pol1_lsu IPR000722, RNA_pol_asu IPR006592, RNA_pol_N IPR007080, RNA_pol_Rpb1_1 IPR007066, RNA_pol_Rpb1_3 IPR042102, RNA_pol_Rpb1_3_sf IPR007083, RNA_pol_Rpb1_4 IPR007081, RNA_pol_Rpb1_5 IPR038120, Rpb1_funnel_sf |
PANTHERi | PTHR19376:SF11, PTHR19376:SF11, 1 hit |
Pfami | View protein in Pfam PF04997, RNA_pol_Rpb1_1, 1 hit PF00623, RNA_pol_Rpb1_2, 1 hit PF04983, RNA_pol_Rpb1_3, 1 hit PF05000, RNA_pol_Rpb1_4, 1 hit PF04998, RNA_pol_Rpb1_5, 1 hit |
SMARTi | View protein in SMART SM00663, RPOLA_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPA1_MOUSE | |
Accessioni | O35134Primary (citable) accession number: O35134 Secondary accession number(s): Q7TSA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | December 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families