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Protein

Ribosomal RNA small subunit methyltransferase NEP1

Gene

Emg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

S-adenosyl-L-methionine-dependent pseudouridine N1-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei86Stabilizes Arg-84By similarity1
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei176S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei201S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei206S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • rRNA (pseudouridine) methyltransferase activity Source: UniProtKB
  • rRNA binding Source: GO_Central

GO - Biological processi

  • blastocyst development Source: MGI
  • nucleologenesis Source: MGI
  • rRNA base methylation Source: GO_Central

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, RNA-binding, rRNA-binding, Transferase
Biological processRibosome biogenesis, rRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase NEP1By similarity (EC:2.1.1.-By similarity)
Alternative name(s):
18S rRNA (pseudouridine(1248)-N1)-methyltransferaseBy similarity
18S rRNA Psi1248 methyltransferaseBy similarity
Nucleolar protein EMG1 homologBy similarity
Protein C2fBy similarity
Ribosome biogenesis protein NEP1By similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Emg1By similarity
Synonyms:C2fBy similarity, Grcc2f
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1315195 Emg1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001586071 – 244Ribosomal RNA small subunit methyltransferase NEP1Add BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei5PhosphoserineBy similarity1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
O35130

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
O35130

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O35130

PeptideAtlas

More...
PeptideAtlasi
O35130

PRoteomics IDEntifications database

More...
PRIDEi
O35130

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O35130

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
O35130

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000004268 Expressed in 306 organ(s), highest expression level in mandibular prominence

CleanEx database of gene expression profiles

More...
CleanExi
MM_EMG1

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
O35130 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
O35130 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei84Interaction with substrate rRNABy similarity1
Sitei125Interaction with substrate rRNABy similarity1
Sitei128Interaction with substrate rRNABy similarity1
Sitei132Interaction with substrate rRNABy similarity1

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
200052, 2 interactors

Protein interaction database and analysis system

More...
IntActi
O35130, 1 interactor

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000004379

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
O35130

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O35130

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni219 – 224S-adenosyl-L-methionine bindingBy similarity6

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3073 Eukaryota
COG1756 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000000305

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000193819

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG029983

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O35130

KEGG Orthology (KO)

More...
KOi
K14568

Identification of Orthologs from Complete Genome Data

More...
OMAi
GCMKIGT

Database of Orthologous Groups

More...
OrthoDBi
1266231at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O35130

TreeFam database of animal gene trees

More...
TreeFami
TF314335

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.1280.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR005304 Rbsml_bgen_MeTrfase_EMG1/NEP1
IPR029026 tRNA_m1G_MTases_N

The PANTHER Classification System

More...
PANTHERi
PTHR12636 PTHR12636, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03587 EMG1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF75217 SSF75217, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35130-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAASGGFQP RERRFSVQEQ DWETTPPKKL RLGAGSKCGG RRLIVVLEGA
60 70 80 90 100
SLETVKVGKT YELLNCDRHK SMLLKNGRDP GEVRPDITHQ SLLMLMDSPL
110 120 130 140 150
NRAGLLQVYI HTQKNVLIEV NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD
160 170 180 190 200
GPQKLLKVIK NPVSDHFPVG CMKIGTSFSV EDISDIRELV PSSDPVVFVV
210 220 230 240
GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKVTTAFEEV WGVI
Length:244
Mass (Da):26,974
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF9B1A6161D30F8BD
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SUL6A0A0N4SUL6_MOUSE
Ribosomal RNA small subunit methylt...
Emg1
122Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36006.1
BC002004 mRNA Translation: AAH02004.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS20524.1

NCBI Reference Sequences

More...
RefSeqi
NP_038564.1, NM_013536.2

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Mm.6390

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000004379; ENSMUSP00000004379; ENSMUSG00000004268

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
14791

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:14791

UCSC genome browser

More...
UCSCi
uc009drg.2 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36006.1
BC002004 mRNA Translation: AAH02004.1
CCDSiCCDS20524.1
RefSeqiNP_038564.1, NM_013536.2
UniGeneiMm.6390

3D structure databases

ProteinModelPortaliO35130
SMRiO35130
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200052, 2 interactors
IntActiO35130, 1 interactor
STRINGi10090.ENSMUSP00000004379

PTM databases

iPTMnetiO35130
PhosphoSitePlusiO35130

Proteomic databases

EPDiO35130
jPOSTiO35130
PaxDbiO35130
PeptideAtlasiO35130
PRIDEiO35130

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
14791
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004379; ENSMUSP00000004379; ENSMUSG00000004268
GeneIDi14791
KEGGimmu:14791
UCSCiuc009drg.2 mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
10436
MGIiMGI:1315195 Emg1

Phylogenomic databases

eggNOGiKOG3073 Eukaryota
COG1756 LUCA
GeneTreeiENSGT00390000000305
HOGENOMiHOG000193819
HOVERGENiHBG029983
InParanoidiO35130
KOiK14568
OMAiGCMKIGT
OrthoDBi1266231at2759
PhylomeDBiO35130
TreeFamiTF314335

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Emg1 mouse

Protein Ontology

More...
PROi
PR:O35130

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000004268 Expressed in 306 organ(s), highest expression level in mandibular prominence
CleanExiMM_EMG1
ExpressionAtlasiO35130 baseline and differential
GenevisibleiO35130 MM

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR005304 Rbsml_bgen_MeTrfase_EMG1/NEP1
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR12636 PTHR12636, 1 hit
PfamiView protein in Pfam
PF03587 EMG1, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNEP1_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O35130
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: January 1, 1998
Last modified: January 16, 2019
This is version 133 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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