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Protein

Ribosomal RNA small subunit methyltransferase NEP1

Gene

Emg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent pseudouridine N1-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.By similarity

Catalytic activityi

S-adenosyl-L-methionine + a pseudouridine in 16S/18S rRNA = S-adenosyl-L-homocysteine + an N1-methylpseudouridine in 16S/18S rRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei86Stabilizes Arg-84By similarity1
Binding sitei176S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei201S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei206S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • rRNA (pseudouridine) methyltransferase activity Source: UniProtKB
  • rRNA binding Source: GO_Central

GO - Biological processi

  • blastocyst development Source: MGI
  • nucleologenesis Source: MGI
  • rRNA base methylation Source: GO_Central

Keywordsi

Molecular functionMethyltransferase, RNA-binding, rRNA-binding, Transferase
Biological processRibosome biogenesis, rRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase NEP1By similarity (EC:2.1.1.-By similarity)
Alternative name(s):
18S rRNA (pseudouridine(1248)-N1)-methyltransferaseBy similarity
18S rRNA Psi1248 methyltransferaseBy similarity
Nucleolar protein EMG1 homologBy similarity
Protein C2fBy similarity
Ribosome biogenesis protein NEP1By similarity
Gene namesi
Name:Emg1By similarity
Synonyms:C2fBy similarity, Grcc2f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315195 Emg1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001586071 – 244Ribosomal RNA small subunit methyltransferase NEP1Add BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35130
PaxDbiO35130
PeptideAtlasiO35130
PRIDEiO35130

PTM databases

iPTMnetiO35130
PhosphoSitePlusiO35130

Expressioni

Gene expression databases

BgeeiENSMUSG00000004268 Expressed in 306 organ(s), highest expression level in mandibular prominence
CleanExiMM_EMG1
ExpressionAtlasiO35130 baseline and differential
GenevisibleiO35130 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei84Interaction with substrate rRNABy similarity1
Sitei125Interaction with substrate rRNABy similarity1
Sitei128Interaction with substrate rRNABy similarity1
Sitei132Interaction with substrate rRNABy similarity1

Protein-protein interaction databases

BioGridi200052, 2 interactors
IntActiO35130, 1 interactor
STRINGi10090.ENSMUSP00000004379

Structurei

3D structure databases

ProteinModelPortaliO35130
SMRiO35130
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 224S-adenosyl-L-methionine bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3073 Eukaryota
COG1756 LUCA
GeneTreeiENSGT00390000000305
HOGENOMiHOG000193819
HOVERGENiHBG029983
InParanoidiO35130
KOiK14568
OMAiGCMKIGT
OrthoDBiEOG091G0PXB
PhylomeDBiO35130
TreeFamiTF314335

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR005304 Rbsml_bgen_MeTrfase_EMG1/NEP1
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR12636 PTHR12636, 1 hit
PfamiView protein in Pfam
PF03587 EMG1, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35130-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAASGGFQP RERRFSVQEQ DWETTPPKKL RLGAGSKCGG RRLIVVLEGA
60 70 80 90 100
SLETVKVGKT YELLNCDRHK SMLLKNGRDP GEVRPDITHQ SLLMLMDSPL
110 120 130 140 150
NRAGLLQVYI HTQKNVLIEV NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD
160 170 180 190 200
GPQKLLKVIK NPVSDHFPVG CMKIGTSFSV EDISDIRELV PSSDPVVFVV
210 220 230 240
GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKVTTAFEEV WGVI
Length:244
Mass (Da):26,974
Last modified:January 1, 1998 - v1
Checksum:iF9B1A6161D30F8BD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SUL6A0A0N4SUL6_MOUSE
Ribosomal RNA small subunit methylt...
Emg1
122Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36006.1
BC002004 mRNA Translation: AAH02004.1
CCDSiCCDS20524.1
RefSeqiNP_038564.1, NM_013536.2
UniGeneiMm.6390

Genome annotation databases

EnsembliENSMUST00000004379; ENSMUSP00000004379; ENSMUSG00000004268
GeneIDi14791
KEGGimmu:14791
UCSCiuc009drg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36006.1
BC002004 mRNA Translation: AAH02004.1
CCDSiCCDS20524.1
RefSeqiNP_038564.1, NM_013536.2
UniGeneiMm.6390

3D structure databases

ProteinModelPortaliO35130
SMRiO35130
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200052, 2 interactors
IntActiO35130, 1 interactor
STRINGi10090.ENSMUSP00000004379

PTM databases

iPTMnetiO35130
PhosphoSitePlusiO35130

Proteomic databases

EPDiO35130
PaxDbiO35130
PeptideAtlasiO35130
PRIDEiO35130

Protocols and materials databases

DNASUi14791
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004379; ENSMUSP00000004379; ENSMUSG00000004268
GeneIDi14791
KEGGimmu:14791
UCSCiuc009drg.2 mouse

Organism-specific databases

CTDi10436
MGIiMGI:1315195 Emg1

Phylogenomic databases

eggNOGiKOG3073 Eukaryota
COG1756 LUCA
GeneTreeiENSGT00390000000305
HOGENOMiHOG000193819
HOVERGENiHBG029983
InParanoidiO35130
KOiK14568
OMAiGCMKIGT
OrthoDBiEOG091G0PXB
PhylomeDBiO35130
TreeFamiTF314335

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiEmg1 mouse
PROiPR:O35130
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004268 Expressed in 306 organ(s), highest expression level in mandibular prominence
CleanExiMM_EMG1
ExpressionAtlasiO35130 baseline and differential
GenevisibleiO35130 MM

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR005304 Rbsml_bgen_MeTrfase_EMG1/NEP1
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR12636 PTHR12636, 1 hit
PfamiView protein in Pfam
PF03587 EMG1, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNEP1_MOUSE
AccessioniPrimary (citable) accession number: O35130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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