UniProtKB - O35115 (FHL2_RAT)
Protein
Four and a half LIM domains protein 2
Gene
Fhl2
Organism
Rattus norvegicus (Rat)
Status
Functioni
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation (By similarity). Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:22851699).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 31 | C4-typeSequence analysisAdd BLAST | 25 |
GO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- identical protein binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- transcription coregulator activity Source: GO_Central
- transcription corepressor activity Source: RGD
- transcription factor binding Source: UniProtKB
GO - Biological processi
- atrial cardiac muscle cell development Source: RGD
- heart trabecula formation Source: RGD
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- osteoblast differentiation Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- response to hormone Source: RGD
- ventricular cardiac muscle cell development Source: RGD
Keywordsi
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Four and a half LIM domains protein 2Short name: FHL-2 Alternative name(s): LIM domain protein DRAL Skeletal muscle LIM-protein 3 Short name: SLIM-3 |
Gene namesi | Name:Fhl2 Synonyms:Dral, Slim3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61963, Fhl2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075739 | 1 – 279 | Four and a half LIM domains protein 2Add BLAST | 279 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 167 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 220 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 238 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | O35115 |
PRIDEi | O35115 |
PTM databases
iPTMneti | O35115 |
PhosphoSitePlusi | O35115 |
Expressioni
Tissue specificityi
Expressed in heart only (at protein level).1 Publication
Gene expression databases
Bgeei | ENSRNOG00000016866, Expressed in heart and 20 other tissues |
Genevisiblei | O35115, RN |
Interactioni
Subunit structurei
Interacts with ZNF638 and TTN/titin.
Interacts with E4F1.
Interacts with GRB7.
Interacts with SIRT1 and FOXO1.
Interacts with CEFIP and calcineurin.
Interacts with FOXK1.
By similarityGO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- identical protein binding Source: RGD
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 248898, 1 interactor |
IntActi | O35115, 1 interactor |
STRINGi | 10116.ENSRNOP00000023014 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 92 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 101 – 153 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 162 – 212 | LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST | 51 | |
Domaini | 221 – 275 | LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST | 55 |
Domaini
The third LIM zinc-binding mediates interaction with E4F1.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 31 | C4-typeSequence analysisAdd BLAST | 25 |
Keywords - Domaini
LIM domain, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1704, Eukaryota |
GeneTreei | ENSGT00950000183028 |
HOGENOMi | CLU_001357_2_0_1 |
InParanoidi | O35115 |
OMAi | KXTRKME |
OrthoDBi | 642235at2759 |
PhylomeDBi | O35115 |
TreeFami | TF321684 |
Family and domain databases
InterProi | View protein in InterPro IPR037987, FHL2/3/5 IPR001781, Znf_LIM |
PANTHERi | PTHR24205, PTHR24205, 1 hit |
Pfami | View protein in Pfam PF00412, LIM, 4 hits |
SMARTi | View protein in SMART SM00132, LIM, 4 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 4 hits PS50023, LIM_DOMAIN_2, 4 hits |
i Sequence
Sequence statusi: Complete.
O35115-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD
60 70 80 90 100
CKDLSYKDRH WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK
110 120 130 140 150
CQECKKTIMP GTRKMEYKGS SWHETCFTCQ RCQQPIGTKS FIPKENQNFC
160 170 180 190 200
VPCYEKQYAL QCVQCKKPIT TGGVTYRDQP WHRECFVCTA CKKQLSGQRF
210 220 230 240 250
TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN
260 270
CKKCSLSLVG RGFLTERDDI LCPDCGKDI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008571 mRNA Translation: BAA23357.1 |
RefSeqi | NP_113865.1, NM_031677.1 XP_006244911.1, XM_006244849.3 XP_017452106.1, XM_017596617.1 |
Genome annotation databases
Ensembli | ENSRNOT00000023014; ENSRNOP00000023014; ENSRNOG00000016866 |
GeneIDi | 63839 |
KEGGi | rno:63839 |
UCSCi | RGD:61963, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008571 mRNA Translation: BAA23357.1 |
RefSeqi | NP_113865.1, NM_031677.1 XP_006244911.1, XM_006244849.3 XP_017452106.1, XM_017596617.1 |
3D structure databases
SMRi | O35115 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248898, 1 interactor |
IntActi | O35115, 1 interactor |
STRINGi | 10116.ENSRNOP00000023014 |
PTM databases
iPTMneti | O35115 |
PhosphoSitePlusi | O35115 |
Proteomic databases
PaxDbi | O35115 |
PRIDEi | O35115 |
Genome annotation databases
Ensembli | ENSRNOT00000023014; ENSRNOP00000023014; ENSRNOG00000016866 |
GeneIDi | 63839 |
KEGGi | rno:63839 |
UCSCi | RGD:61963, rat |
Organism-specific databases
CTDi | 2274 |
RGDi | 61963, Fhl2 |
Phylogenomic databases
eggNOGi | KOG1704, Eukaryota |
GeneTreei | ENSGT00950000183028 |
HOGENOMi | CLU_001357_2_0_1 |
InParanoidi | O35115 |
OMAi | KXTRKME |
OrthoDBi | 642235at2759 |
PhylomeDBi | O35115 |
TreeFami | TF321684 |
Miscellaneous databases
PROi | PR:O35115 |
Gene expression databases
Bgeei | ENSRNOG00000016866, Expressed in heart and 20 other tissues |
Genevisiblei | O35115, RN |
Family and domain databases
InterProi | View protein in InterPro IPR037987, FHL2/3/5 IPR001781, Znf_LIM |
PANTHERi | PTHR24205, PTHR24205, 1 hit |
Pfami | View protein in Pfam PF00412, LIM, 4 hits |
SMARTi | View protein in SMART SM00132, LIM, 4 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 4 hits PS50023, LIM_DOMAIN_2, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FHL2_RAT | |
Accessioni | O35115Primary (citable) accession number: O35115 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |