Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysosome membrane protein 2

Gene

Scarb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.2 Publications

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • scavenger receptor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionReceptor

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Protein family/group databases

TCDBi9.B.39.1.9 the long chain fatty acid translocase (lcfat) family

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome membrane protein 2
Alternative name(s):
85 kDa lysosomal membrane sialoglycoprotein
Short name:
LGP85
Lysosome membrane protein II
Short name:
LIMP II
Scavenger receptor class B member 2
Gene namesi
Name:Scarb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1196458 Scarb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 27HelicalSequence analysisAdd BLAST23
Topological domaini28 – 433LumenalSequence analysisAdd BLAST406
Transmembranei434 – 459HelicalSequence analysisAdd BLAST26
Topological domaini460 – 478CytoplasmicSequence analysisAdd BLAST19

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi68N → Q: Loss of glycosylation site. Causes retention in the endoplasmic reticulum and abolishes normal location in lysosomes. 1 Publication1
Mutagenesisi105N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi122N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi155L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi156I → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi159M → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi160L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi162A → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi163Y → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi166K → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi184I → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi187L → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi191F → D: Abolishes interaction with GBA. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi206N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi224N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi249N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi304N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi325N → Q: Loss of glycosylation site. Causes retention in the endoplasmic reticulum and abolishes normal location in lysosomes. 1 Publication1
Mutagenesisi400D → K: Slightly increased GBA binding. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi412N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1
Mutagenesisi430N → Q: Loss of glycosylation site. No effect on normal location in lysosomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001441561 – 478Lysosome membrane protein 2Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi105N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi224N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi274 ↔ 329By similarity
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi312 ↔ 318By similarity
Glycosylationi325N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi412N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi430N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Acylated by palmitic acid group(s).By similarity
Heavily glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

EPDiO35114
MaxQBiO35114
PaxDbiO35114
PeptideAtlasiO35114
PRIDEiO35114

PTM databases

iPTMnetiO35114
PhosphoSitePlusiO35114
SwissPalmiO35114

Expressioni

Tissue specificityi

Detected in the extracts of brain, heart, lung, liver and kidney.

Gene expression databases

BgeeiENSMUSG00000029426 Expressed in 296 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_SCARB2
GenevisibleiO35114 MM

Interactioni

Subunit structurei

Interacts with GBA.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35114, 6 interactors
MINTiO35114
STRINGi10090.ENSMUSP00000031377

Structurei

3D structure databases

ProteinModelPortaliO35114
SMRiO35114
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 191Important for interaction with GBAAdd BLAST37

Sequence similaritiesi

Belongs to the CD36 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3776 Eukaryota
ENOG410XS17 LUCA
GeneTreeiENSGT00530000062927
HOGENOMiHOG000252951
HOVERGENiHBG106707
InParanoidiO35114
KOiK12384
OMAiYHETFVD
OrthoDBiEOG091G0CH9
PhylomeDBiO35114
TreeFamiTF317925

Family and domain databases

InterProiView protein in InterPro
IPR002159 CD36_fam
IPR005429 LimpII
PANTHERiPTHR11923 PTHR11923, 1 hit
PfamiView protein in Pfam
PF01130 CD36, 1 hit
PRINTSiPR01609 CD36FAMILY
PR01611 LIMPII

Sequencei

Sequence statusi: Complete.

O35114-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRCCFYTAG TLSLLLLVTS VTLLVARVFQ KAVDQTIEKN MVLQNGTKVF
60 70 80 90 100
NSWEKPPLPV YIQFYFFNVT NPEEILQGEI PLLEEVGPYT YRELRNKANI
110 120 130 140 150
QFGENGTTIS AVTNKAYVFE RNQSVGDPNV DLIRTINIPL LTVVDLAQLT
160 170 180 190 200
LLRELIEAML KAYQQKLFVI HTVHELLWGY KDEILSLVHI FKPDVSPNFG
210 220 230 240 250
LFYERNGTND GEYVFLTGED NYLNFSKIVE WNGKTSLDWW TTDTCNMING
260 270 280 290 300
TDGDSFHPLI SKDEVLYLFP SDLCRSVHIT FSSFENVEGL PAFRYKVPAE
310 320 330 340 350
ILANTSENAG FCIPEGNCMD SGVLNISICK NGAPIIMSFP HFYQADEKFV
360 370 380 390 400
SAIKGMHPNK EEHESFVDIN PLTGIILRGA KRFQINTYVR KLDDFVETGD
410 420 430 440 450
IRTMVFPVMY LNESVLIDKE TANQLKSVIN TTLVVTNIPY IIMALGVFFG
460 470
LVFTWLACRG QGSMDEGTAD ERAPLIRT
Length:478
Mass (Da):54,044
Last modified:January 23, 2007 - v3
Checksum:i55724B77855470DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008553 mRNA Translation: BAA23372.1
AK011123 mRNA Translation: BAB27416.1
AK083038 mRNA Translation: BAC38740.1
AK144235 mRNA Translation: BAE25789.1
BC029073 mRNA Translation: AAH29073.1
CCDSiCCDS19431.1
PIRiJC5670
RefSeqiNP_031670.1, NM_007644.4
UniGeneiMm.297964

Genome annotation databases

EnsembliENSMUST00000031377; ENSMUSP00000031377; ENSMUSG00000029426
GeneIDi12492
KEGGimmu:12492
UCSCiuc008ydm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008553 mRNA Translation: BAA23372.1
AK011123 mRNA Translation: BAB27416.1
AK083038 mRNA Translation: BAC38740.1
AK144235 mRNA Translation: BAE25789.1
BC029073 mRNA Translation: AAH29073.1
CCDSiCCDS19431.1
PIRiJC5670
RefSeqiNP_031670.1, NM_007644.4
UniGeneiMm.297964

3D structure databases

ProteinModelPortaliO35114
SMRiO35114
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35114, 6 interactors
MINTiO35114
STRINGi10090.ENSMUSP00000031377

Protein family/group databases

TCDBi9.B.39.1.9 the long chain fatty acid translocase (lcfat) family

PTM databases

iPTMnetiO35114
PhosphoSitePlusiO35114
SwissPalmiO35114

Proteomic databases

EPDiO35114
MaxQBiO35114
PaxDbiO35114
PeptideAtlasiO35114
PRIDEiO35114

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031377; ENSMUSP00000031377; ENSMUSG00000029426
GeneIDi12492
KEGGimmu:12492
UCSCiuc008ydm.1 mouse

Organism-specific databases

CTDi950
MGIiMGI:1196458 Scarb2

Phylogenomic databases

eggNOGiKOG3776 Eukaryota
ENOG410XS17 LUCA
GeneTreeiENSGT00530000062927
HOGENOMiHOG000252951
HOVERGENiHBG106707
InParanoidiO35114
KOiK12384
OMAiYHETFVD
OrthoDBiEOG091G0CH9
PhylomeDBiO35114
TreeFamiTF317925

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:O35114
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029426 Expressed in 296 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_SCARB2
GenevisibleiO35114 MM

Family and domain databases

InterProiView protein in InterPro
IPR002159 CD36_fam
IPR005429 LimpII
PANTHERiPTHR11923 PTHR11923, 1 hit
PfamiView protein in Pfam
PF01130 CD36, 1 hit
PRINTSiPR01609 CD36FAMILY
PR01611 LIMPII
ProtoNetiSearch...

Entry informationi

Entry nameiSCRB2_MOUSE
AccessioniPrimary (citable) accession number: O35114
Secondary accession number(s): Q3UNF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again