Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prostacyclin synthase

Gene

Ptgis

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).By similarity

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107SubstrateBy similarity1
Binding sitei113Substrate; via amide nitrogenBy similarity1
Binding sitei288SubstrateBy similarity1
Binding sitei383SubstrateBy similarity1
Metal bindingi442Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-MMU-197264 Nicotinamide salvaging
R-MMU-211979 Eicosanoids
R-MMU-211994 Sterols are 12-hydroxylated by CYP8B1
R-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4By similarity)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:Ptgis
Synonyms:Cyp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1097156 Ptgis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519111 – 501Prostacyclin synthaseAdd BLAST501

Proteomic databases

MaxQBiO35074
PaxDbiO35074
PeptideAtlasiO35074
PRIDEiO35074

PTM databases

iPTMnetiO35074
PhosphoSitePlusiO35074

Expressioni

Gene expression databases

BgeeiENSMUSG00000017969 Expressed in 239 organ(s), highest expression level in aorta
CleanExiMM_PTGIS
ExpressionAtlasiO35074 baseline and differential
GenevisibleiO35074 MM

Interactioni

Protein-protein interaction databases

BioGridi202461, 1 interactor
IntActiO35074, 1 interactor
MINTiO35074
STRINGi10090.ENSMUSP00000018113

Structurei

3D structure databases

ProteinModelPortaliO35074
SMRiO35074
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 360Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00550000074551
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiO35074
KOiK01831
OMAiWATQGNV
TreeFamiTF105090

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR027286 PTGIS
PANTHERiPTHR24306:SF4 PTHR24306:SF4, 1 hit
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PIRSF500628 PTGIS, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O35074-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGRD
60 70 80 90 100
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWE LRTRLDFHPY
110 120 130 140 150
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HRDLQALTEA MYTNLRTVLL
160 170 180 190 200
GDSTEAGSGW QETGLLEFSY NALLSAGYLT LYGVEASPRT HESQAQDRVH
210 220 230 240 250
SADVFHTFRQ LDLLLPKLAR GSLSAGDKDH ACSVKNRLWK LLSPARLASR
260 270 280 290 300
ADRSSWLESY LRHLEEMGVS EEMQARALVL QLWATQGNMG PTAFWLLLFL
310 320 330 340 350
LKNPEALAAV RAELKHTVWQ AEQPVSQMTT LPQKILDSMP VLDSVLNETL
360 370 380 390 400
RLTAAPFITR EVMADLALPM ADGREFSLRR GDRLLLFPFL SPQKDPEIYT
410 420 430 440 450
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN
460 470 480 490 500
SIKQFVVLLL THFDLELGSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRAR

L
Length:501
Mass (Da):57,047
Last modified:January 1, 1998 - v1
Checksum:iF018F85D3A1B0EBB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BXC0Q8BXC0_MOUSE
Prostacyclin synthase
Ptgis mCG_14612
509Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001607 mRNA Translation: BAA21717.1
AK076585 mRNA Translation: BAC36403.1
BC062151 mRNA Translation: AAH62151.1
CCDSiCCDS17097.1
RefSeqiNP_032994.1, NM_008968.3
UniGeneiMm.2339

Genome annotation databases

EnsembliENSMUST00000018113; ENSMUSP00000018113; ENSMUSG00000017969
GeneIDi19223
KEGGimmu:19223
UCSCiuc008nzl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001607 mRNA Translation: BAA21717.1
AK076585 mRNA Translation: BAC36403.1
BC062151 mRNA Translation: AAH62151.1
CCDSiCCDS17097.1
RefSeqiNP_032994.1, NM_008968.3
UniGeneiMm.2339

3D structure databases

ProteinModelPortaliO35074
SMRiO35074
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202461, 1 interactor
IntActiO35074, 1 interactor
MINTiO35074
STRINGi10090.ENSMUSP00000018113

PTM databases

iPTMnetiO35074
PhosphoSitePlusiO35074

Proteomic databases

MaxQBiO35074
PaxDbiO35074
PeptideAtlasiO35074
PRIDEiO35074

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018113; ENSMUSP00000018113; ENSMUSG00000017969
GeneIDi19223
KEGGimmu:19223
UCSCiuc008nzl.1 mouse

Organism-specific databases

CTDi5740
MGIiMGI:1097156 Ptgis

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00550000074551
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiO35074
KOiK01831
OMAiWATQGNV
TreeFamiTF105090

Enzyme and pathway databases

ReactomeiR-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-MMU-197264 Nicotinamide salvaging
R-MMU-211979 Eicosanoids
R-MMU-211994 Sterols are 12-hydroxylated by CYP8B1
R-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Miscellaneous databases

PROiPR:O35074
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017969 Expressed in 239 organ(s), highest expression level in aorta
CleanExiMM_PTGIS
ExpressionAtlasiO35074 baseline and differential
GenevisibleiO35074 MM

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR027286 PTGIS
PANTHERiPTHR24306:SF4 PTHR24306:SF4, 1 hit
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PIRSF500628 PTGIS, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTGIS_MOUSE
AccessioniPrimary (citable) accession number: O35074
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again