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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC), Histidinol-phosphate aminotransferase (hisC)
  8. Histidinol-phosphatase (hisK)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton acceptorBy similarity1
Active sitei132Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU34880-MONOMER
UniPathwayiUPA00031; UER00009

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:hisA
Ordered Locus Names:BSU34880
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001419781 – 2451-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomeraseAdd BLAST245

Proteomic databases

PaxDbiO35006
PRIDEiO35006

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100018886

Structurei

3D structure databases

ProteinModelPortaliO35006
SMRiO35006
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGiENOG4105CJV Bacteria
COG0106 LUCA
HOGENOMiHOG000224614
InParanoidiO35006
KOiK01814
OMAiEWLHLVD
PhylomeDBiO35006

Family and domain databases

CDDicd04732 HisA, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01014 HisA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006062 His_biosynth
IPR006063 HisA
IPR023016 Isoase_HisA
IPR011060 RibuloseP-bd_barrel
PfamiView protein in Pfam
PF00977 His_biosynth, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00007 TIGR00007, 1 hit

Sequencei

Sequence statusi: Complete.

O35006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFTLYPAI DMRNGKCVRL VQGDYNKETI YGDSPYDMAE LFEKEGAEWI
60 70 80 90 100
HLVDLDGAKE GKRVNDRHVI EIAQKLNLKV EIGGGIRSEN DVYEYLSAGV
110 120 130 140 150
ERVILGSSAV SNPPFVKKML KQYGEKIAIG LDARNGFVST EGWLETSTLK
160 170 180 190 200
ATELGKELAN EGAEVFIFTD IATDGMLSGP NVKSTVELAK ETGKSVIASG
210 220 230 240
GVSSVADLEA LARNEADGVS GAIIGKALYT NQFTLSEALE RVKRK
Length:245
Mass (Da):26,529
Last modified:January 1, 1998 - v1
Checksum:i94BC1413092C93F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017113 Genomic DNA Translation: AAC67298.1
AL009126 Genomic DNA Translation: CAB15493.1
PIRiF69640
RefSeqiNP_391368.1, NC_000964.3
WP_003242559.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15493; CAB15493; BSU34880
GeneIDi936580
KEGGibsu:BSU34880
PATRICifig|224308.179.peg.3776

Similar proteinsi

Entry informationi

Entry nameiHIS4_BACSU
AccessioniPrimary (citable) accession number: O35006
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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