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UniProtKB - O34996 (DPO1_BACSU)
Protein
DNA polymerase I
Gene
polA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.
By similarityCatalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: GO_Central
GO - Biological processi
- DNA-dependent DNA replication Source: InterPro
- double-strand break repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Enzyme and pathway databases
BioCyci | BSUB:BSU29090-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polA Ordered Locus Names:BSU29090 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101235 | 1 – 880 | DNA polymerase IAdd BLAST | 880 |
Proteomic databases
PaxDbi | O34996 |
PRIDEi | O34996 |
Interactioni
Subunit structurei
Single-chain monomer with multiple functions.
Protein-protein interaction databases
IntActi | O34996, 8 interactors |
STRINGi | 224308.BSU29090 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 174 – 268 | 5'-3' exonucleaseSequence analysisAdd BLAST | 95 | |
Domaini | 302 – 470 | 3'-5' exonucleaseSequence analysisAdd BLAST | 169 |
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Phylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
InParanoidi | O34996 |
OMAi | NRPPMPD |
PhylomeDBi | O34996 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 2 hits |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
O34996-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTERKKLVLV DGNSLAYRAF FALPLLSNDK GVHTNAVYGF AMILMKMLED
60 70 80 90 100
EKPTHMLVAF DAGKTTFRHG TFKEYKGGRQ KTPPELSEQM PFIRELLDAY
110 120 130 140 150
QISRYELEQY EADDIIGTLA KSAEKDGFEV KVFSGDKDLT QLATDKTTVA
160 170 180 190 200
ITRKGITDVE FYTPEHVKEK YGLTPEQIID MKGLMGDSSD NIPGVPGVGE
210 220 230 240 250
KTAIKLLKQF DSVEKLLESI DEVSGKKLKE KLEEFKDQAL MSKELATIMT
260 270 280 290 300
DAPIEVSVSG LEYQGFNREQ VIAIFKDLGF NTLLERLGED SAEAEQDQSL
310 320 330 340 350
EDINVKTVTD VTSDILVSPS AFVVEQIGDN YHEEPILGFS IVNETGAYFI
360 370 380 390 400
PKDIAVESEV FKEWVENDEQ KKWVFDSKRA VVALRWQGIE LKGAEFDTLL
410 420 430 440 450
AAYIINPGNS YDDVASVAKD YGLHIVSSDE SVYGKGAKRA VPSEDVLSEH
460 470 480 490 500
LGRKALAIQS LREKLVQELE NNDQLELFEE LEMPLALILG EMESTGVKVD
510 520 530 540 550
VDRLKRMGEE LGAKLKEYEE KIHEIAGEPF NINSPKQLGV ILFEKIGLPV
560 570 580 590 600
VKKTKTGYST SADVLEKLAD KHDIVDYILQ YRQIGKLQST YIEGLLKVTR
610 620 630 640 650
PDSHKVHTRF NQALTQTGRL SSTDPNLQNI PIRLEEGRKI RQAFVPSEKD
660 670 680 690 700
WLIFAADYSQ IELRVLAHIS KDENLIEAFT NDMDIHTKTA MDVFHVAKDE
710 720 730 740 750
VTSAMRRQAK AVNFGIVYGI SDYGLSQNLG ITRKEAGAFI DRYLESFQGV
760 770 780 790 800
KAYMEDSVQE AKQKGYVTTL MHRRRYIPEL TSRNFNIRSF AERTAMNTPI
810 820 830 840 850
QGSAADIIKK AMIDMAAKLK EKQLKARLLL QVHDELIFEA PKEEIEILEK
860 870 880
LVPEVMEHAL ALDVPLKVDF ASGPSWYDAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00350.1 AL009126 Genomic DNA Translation: CAB14869.1 |
PIRi | E69680 |
RefSeqi | NP_390787.1, NC_000964.3 WP_004398870.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14869; CAB14869; BSU_29090 |
GeneIDi | 936617 |
KEGGi | bsu:BSU29090 |
PATRICi | fig|224308.179.peg.3158 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00350.1 AL009126 Genomic DNA Translation: CAB14869.1 |
PIRi | E69680 |
RefSeqi | NP_390787.1, NC_000964.3 WP_004398870.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | O34996 |
SMRi | O34996 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O34996, 8 interactors |
STRINGi | 224308.BSU29090 |
Proteomic databases
PaxDbi | O34996 |
PRIDEi | O34996 |
Genome annotation databases
EnsemblBacteriai | CAB14869; CAB14869; BSU_29090 |
GeneIDi | 936617 |
KEGGi | bsu:BSU29090 |
PATRICi | fig|224308.179.peg.3158 |
Phylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
InParanoidi | O34996 |
OMAi | NRPPMPD |
PhylomeDBi | O34996 |
Enzyme and pathway databases
BioCyci | BSUB:BSU29090-MONOMER |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 2 hits |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO1_BACSU | |
Accessioni | O34996Primary (citable) accession number: O34996 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families