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UniProtKB - O34990 (PURU_BACSU)
Protein
Formyltetrahydrofolate deformylase
Gene
purU
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).
UniRule annotationCatalytic activityi
- (6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-tetrahydrofolate + formate + H+UniRule annotationEC:3.5.1.10UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate.UniRule annotation This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from 10-formyl-5,6,7,8-tetrahydrofolate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 244 | UniRule annotation | 1 |
GO - Molecular functioni
- formyltetrahydrofolate deformylase activity Source: UniProtKB-UniRule
- hydroxymethyl-, formyl- and related transferase activity Source: InterPro
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
- one-carbon metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | One-carbon metabolism, Purine biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU13110-MONOMER |
UniPathwayi | UPA00074;UER00170 |
Names & Taxonomyi
Protein namesi | Recommended name: Formyltetrahydrofolate deformylaseUniRule annotation (EC:3.5.1.10UniRule annotation)Alternative name(s): Formyl-FH(4) hydrolaseUniRule annotation |
Gene namesi | Name:purUUniRule annotation Synonyms:ykkE Ordered Locus Names:BSU13110 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360820 | 1 – 300 | Formyltetrahydrofolate deformylaseAdd BLAST | 300 |
Proteomic databases
jPOSTi | O34990 |
PaxDbi | O34990 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 102 | ACTUniRule annotationAdd BLAST | 82 |
Sequence similaritiesi
Belongs to the PurU family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0788, Bacteria |
InParanoidi | O34990 |
OMAi | HADHHTD |
PhylomeDBi | O34990 |
Family and domain databases
CDDi | cd04875, ACT_F4HF-DF, 1 hit cd08648, FMT_core_Formyl-FH4-Hydrolase_C, 1 hit |
HAMAPi | MF_01927, PurU, 1 hit |
InterProi | View protein in InterPro IPR045865, ACT-like_dom_sf IPR002912, ACT_dom IPR041729, Formyl-FH4-Hydrolase_C IPR002376, Formyl_transf_N IPR036477, Formyl_transf_N_sf IPR004810, PurU IPR044074, PurU_ACT |
PANTHERi | PTHR42706, PTHR42706, 1 hit |
Pfami | View protein in Pfam PF01842, ACT, 1 hit PF00551, Formyl_trans_N, 1 hit |
PRINTSi | PR01575, FFH4HYDRLASE |
SUPFAMi | SSF53328, SSF53328, 1 hit SSF55021, SSF55021, 1 hit |
TIGRFAMsi | TIGR00655, PurU, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit |
i Sequence
Sequence statusi: Complete.
O34990-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSYMTQRLD EYRDGNEDKG RLLVSCPDQP GIVSAVSAFL FEHGANIIES
60 70 80 90 100
NQYTTDPEGG RFFLRIEFDC AGIREKKSSL QAAFASVAEK FDMTWSLTLA
110 120 130 140 150
SELKRVAIFV SKELHCLHEL IWEWQTGNLM AEIAVVISNH EEARELVERL
160 170 180 190 200
NIPFHYMKAN KDIRAEVEKK QLELLEQYDV DVIVLARYMQ ILTPDFVSAH
210 220 230 240 250
PNRIINIHHS FLPAFIGANP YKRAYERGVK LIGATSHYVT NDLDEGPIIE
260 270 280 290 300
QDIERVDHRD NAEALKNIGR TIERSVLARA VKWHLEDRVI VHENKTIVFN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 113 | E → N in CAA05590 (Ref. 1) Curated | 1 | |
Sequence conflicti | 254 | E → K in CAA05590 (Ref. 1) Curated | 1 | |
Sequence conflicti | 264 | A → T in CAA05590 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05590.1 AL009126 Genomic DNA Translation: CAB13168.2 |
PIRi | C69857 |
RefSeqi | NP_389194.2, NC_000964.3 WP_003245802.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13168; CAB13168; BSU_13110 |
GeneIDi | 939854 |
KEGGi | bsu:BSU13110 |
PATRICi | fig|224308.179.peg.1423 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05590.1 AL009126 Genomic DNA Translation: CAB13168.2 |
PIRi | C69857 |
RefSeqi | NP_389194.2, NC_000964.3 WP_003245802.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | O34990 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU13110 |
Proteomic databases
jPOSTi | O34990 |
PaxDbi | O34990 |
Genome annotation databases
EnsemblBacteriai | CAB13168; CAB13168; BSU_13110 |
GeneIDi | 939854 |
KEGGi | bsu:BSU13110 |
PATRICi | fig|224308.179.peg.1423 |
Phylogenomic databases
eggNOGi | COG0788, Bacteria |
InParanoidi | O34990 |
OMAi | HADHHTD |
PhylomeDBi | O34990 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00170 |
BioCyci | BSUB:BSU13110-MONOMER |
Family and domain databases
CDDi | cd04875, ACT_F4HF-DF, 1 hit cd08648, FMT_core_Formyl-FH4-Hydrolase_C, 1 hit |
HAMAPi | MF_01927, PurU, 1 hit |
InterProi | View protein in InterPro IPR045865, ACT-like_dom_sf IPR002912, ACT_dom IPR041729, Formyl-FH4-Hydrolase_C IPR002376, Formyl_transf_N IPR036477, Formyl_transf_N_sf IPR004810, PurU IPR044074, PurU_ACT |
PANTHERi | PTHR42706, PTHR42706, 1 hit |
Pfami | View protein in Pfam PF01842, ACT, 1 hit PF00551, Formyl_trans_N, 1 hit |
PRINTSi | PR01575, FFH4HYDRLASE |
SUPFAMi | SSF53328, SSF53328, 1 hit SSF55021, SSF55021, 1 hit |
TIGRFAMsi | TIGR00655, PurU, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PURU_BACSU | |
Accessioni | O34990Primary (citable) accession number: O34990 Secondary accession number(s): Q796M2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | June 16, 2009 | |
Last modified: | February 23, 2022 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families