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UniProtKB - O34874 (ISCS2_BACSU)
Protein
Putative cysteine desulfurase IscS 2
Gene
iscS2
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the removal of elemental sulfur from cysteine to produce alanine.
By similarityCatalytic activityi
- EC:2.8.1.7By similarity
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 150 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 179 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 237 | Pyridoxal phosphateBy similarity | 1 | |
Active sitei | 324 | Cysteine persulfide intermediateBy similarity | 1 | |
Metal bindingi | 324 | Iron-sulfur (2Fe-2S); via persulfide groupBy similarity | 1 |
GO - Molecular functioni
- cysteine desulfurase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Ligand | Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | BSUB:BSU29590-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:iscS2 Synonyms:nifS2 Ordered Locus Names:BSU29590 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000386537 | 1 – 381 | Putative cysteine desulfurase IscS 2Add BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 202 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | O34874 |
PRIDEi | O34874 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 70 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 199 – 201 | Pyridoxal phosphate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.Curated
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
InParanoidi | O34874 |
OMAi | APHIINF |
PhylomeDBi | O34874 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
O34874-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIYLDNSSTT KPYDEVLNVY EQTSSRYFGN PSSLHRYGAE TEQLLQAAKN
60 70 80 90 100
QIKRSLGLKK YDIVFTSGAT EANNAALKGA ALSKIKTGKH IIATSIEHPS
110 120 130 140 150
VTESLEQLKE LFGFDITYLS VNEDGFVSIE DLKAAIRPDT VLVSMMHVNN
160 170 180 190 200
EVGSVQPIEA AGEVLKEHSN ILFHVDYVQG IYKVPLAIEK AGIDLCSISG
210 220 230 240 250
HKFHGLKGTG ALIVKEGTRL IPLITGGSQQ KGIRAGTEHT AGAVSLAKAI
260 270 280 290 300
NLASADFDTR LDTMTAVKEL FMNRLSEIEG VVINTPQMNS APHIINFSVP
310 320 330 340 350
GIKAEVLLHM LEEQDIFVST TSACSAKEHK PSKVLLEMGK GEQIAGSSIR
360 370 380
ISLNYSQTSD VAEPFMNALR PGIKKLREMM R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00307.1 AL009126 Genomic DNA Translation: CAB14937.1 |
PIRi | F69666 |
RefSeqi | NP_390837.1, NC_000964.3 WP_004398687.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14937; CAB14937; BSU_29590 |
GeneIDi | 937701 |
KEGGi | bsu:BSU29590 |
PATRICi | fig|224308.179.peg.3215 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00307.1 AL009126 Genomic DNA Translation: CAB14937.1 |
PIRi | F69666 |
RefSeqi | NP_390837.1, NC_000964.3 WP_004398687.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | O34874 |
SMRi | O34874 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU29590 |
Proteomic databases
PaxDbi | O34874 |
PRIDEi | O34874 |
Genome annotation databases
EnsemblBacteriai | CAB14937; CAB14937; BSU_29590 |
GeneIDi | 937701 |
KEGGi | bsu:BSU29590 |
PATRICi | fig|224308.179.peg.3215 |
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
InParanoidi | O34874 |
OMAi | APHIINF |
PhylomeDBi | O34874 |
Enzyme and pathway databases
BioCyci | BSUB:BSU29590-MONOMER |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISCS2_BACSU | |
Accessioni | O34874Primary (citable) accession number: O34874 Secondary accession number(s): Q795T7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 13, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families