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UniProtKB - O34859 (KU_BACSU)
Protein
Non-homologous end joining protein Ku
Gene
ku
Organism
Bacillus subtilis (strain 168)
Status
Functioni
With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD (By similarity).
Probably involved in DNA repair during spore germination.
UniRule annotation1 PublicationGO - Molecular functioni
- double-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA recombination Source: UniProtKB-KW
- double-strand break repair via nonhomologous end joining Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair |
Enzyme and pathway databases
BioCyci | BSUB:BSU13410-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Non-homologous end joining protein KuUniRule annotation |
Gene namesi | Name:kuUniRule annotation Synonyms:ykoV Ordered Locus Names:BSU13410 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Other locations
- Spore core 1 Publication
Note: Expressed in the center of the spore core, presumably with the nucleoid.
Pathology & Biotechi
Disruption phenotypei
Stationary-phase cells lacking this gene are more sensitive to ionizing radiation. Growth rate, ultraviolet light and methyl-methanesulfonate sensitivities are unaffected. The spores formed are significantly more sensitive to dry heat, high vacuum-induced desiccation, X-rays and UV radiation, but no difference in hydrogen peroxide resistance exists.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000389176 | 1 – 311 | Non-homologous end joining protein KuAdd BLAST | 311 |
Proteomic databases
PaxDbi | O34859 |
Expressioni
Developmental stagei
Expressed in sporulating cells. Disappears after 90 minutes of spore germination.1 Publication
Inductioni
Transcriptionally regulated by SpoVT and sigma-G factor.1 Publication
Interactioni
Subunit structurei
Homodimer.
Interacts with LigD.
UniRule annotationProtein-protein interaction databases
STRINGi | 224308.BSU13410 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 210 | KuUniRule annotationAdd BLAST | 185 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 269 – 311 | DisorderedSequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Belongs to the prokaryotic Ku family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1273, Bacteria |
InParanoidi | O34859 |
OMAi | IRYRKVC |
PhylomeDBi | O34859 |
Family and domain databases
CDDi | cd00789, KU_like, 1 hit |
Gene3Di | 2.40.290.10, 1 hit |
HAMAPi | MF_01875, Prokaryotic_Ku, 1 hit |
InterProi | View protein in InterPro IPR006164, Ku70/Ku80_beta-barrel_dom IPR009187, Prok_Ku IPR016194, SPOC-like_C_dom_sf |
PANTHERi | PTHR41251, PTHR41251, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit |
PIRSFi | PIRSF006493, Prok_Ku, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit |
TIGRFAMsi | TIGR02772, Ku_bact, 1 hit |
i Sequence
Sequence statusi: Complete.
O34859-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNRTPSLHTK EKKGFIDMHT MWKGSISFGL VNIPIKLYAA TEDKDIKLRS
60 70 80 90 100
LHKEDHAPIK YEKVCTNCEK TLSPDEIVKG YEYVKGKYVV LTDEDLKSLK
110 120 130 140 150
QEHEEKAVEI VDFVQLQEID PIYFNRSYFV GPGDNGTKAY TLLREALRST
160 170 180 190 200
GKIGIANMTI RSKQQLAILR VYENCIVMES IHYPDEVRSA AQVPGVPDQS
210 220 230 240 250
NVNDKELQTA ITLIDELTAK FEPEKYEDTY RQALLQRVND KLENKETAVT
260 270 280 290 300
PDKAPPREDV IDLVSALQAS IDRTRRPNRE TPAAAPAQAA EPKGAGDKKQ
310
KTTRKKASGT S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13198.1 |
PIRi | H69860 |
RefSeqi | NP_389224.1, NC_000964.3 WP_010886496.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13198; CAB13198; BSU_13410 |
GeneIDi | 939375 |
KEGGi | bsu:BSU13410 |
PATRICi | fig|224308.43.peg.1414 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13198.1 |
PIRi | H69860 |
RefSeqi | NP_389224.1, NC_000964.3 WP_010886496.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | O34859 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU13410 |
Proteomic databases
PaxDbi | O34859 |
Protocols and materials databases
DNASUi | 939375 |
Genome annotation databases
EnsemblBacteriai | CAB13198; CAB13198; BSU_13410 |
GeneIDi | 939375 |
KEGGi | bsu:BSU13410 |
PATRICi | fig|224308.43.peg.1414 |
Phylogenomic databases
eggNOGi | COG1273, Bacteria |
InParanoidi | O34859 |
OMAi | IRYRKVC |
PhylomeDBi | O34859 |
Enzyme and pathway databases
BioCyci | BSUB:BSU13410-MONOMER |
Family and domain databases
CDDi | cd00789, KU_like, 1 hit |
Gene3Di | 2.40.290.10, 1 hit |
HAMAPi | MF_01875, Prokaryotic_Ku, 1 hit |
InterProi | View protein in InterPro IPR006164, Ku70/Ku80_beta-barrel_dom IPR009187, Prok_Ku IPR016194, SPOC-like_C_dom_sf |
PANTHERi | PTHR41251, PTHR41251, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit |
PIRSFi | PIRSF006493, Prok_Ku, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit |
TIGRFAMsi | TIGR02772, Ku_bact, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KU_BACSU | |
Accessioni | O34859Primary (citable) accession number: O34859 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 24, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | June 2, 2021 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families