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UniProtKB - O34777 (OHRR_BACSU)
Protein
Organic hydroperoxide resistance transcriptional regulator
Gene
ohrR
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Organic peroxide sensor (PubMed:11418552).
Represses the expression of the peroxide-inducible gene ohrA by cooperative binding to two inverted repeat elements (PubMed:11418552, PubMed:24313874).
2 PublicationsActivity regulationi
Inactivated by oxidation of Cys-15 to a sulfenic acid.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 57 – 80 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- response to hydrogen peroxide Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU13150-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Organic hydroperoxide resistance transcriptional regulator |
Gene namesi | Name:ohrR Synonyms:ykmA Ordered Locus Names:BSU13150 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | C → G or S: Full repressor activity, but no modulation by peroxide. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054376 | 1 – 147 | Organic hydroperoxide resistance transcriptional regulatorAdd BLAST | 147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 15 ↔ 16 | N,N-(cysteine-1,S-diyl)phenylalanine (Cys-Phe); alternate | ||
Modified residuei | 15 | Cysteine sulfenic acid (-SOH); alternate1 Publication | 1 | |
Modified residuei | 15 | S-bacillithiol cysteine disulfide; alternate2 Publications | 1 | |
Modified residuei | 15 | S-cysteinyl cysteine; alternate1 Publication | 1 |
Post-translational modificationi
Cys-15 is oxidized by organic peroxides to cysteine sulfenic acid (Cys-SOH). This can react with the alpha-amido of the following residue to form the sulfenamide cross-link. Oxidation or cross-linking results in the loss of DNA-binding activity and the inactivation of repressor function. Both the cysteine sulfenic acid and the sulfenamide cross-link can react with free cysteine or bacillithiol (BSH) to form mixed disulfides. Further reduction of OhrR by free sulfhydryl compounds restores repressor activity.5 Publications
Keywords - PTMi
OxidationProteomic databases
PaxDbi | O34777 |
PRIDEi | O34777 |
Interactioni
Subunit structurei
Homodimer.
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 224308.BSU13150 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O34777 |
SMRi | O34777 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O34777 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 141 | HTH marR-typePROSITE-ProRule annotationAdd BLAST | 131 |
Phylogenomic databases
eggNOGi | COG1846, Bacteria |
InParanoidi | O34777 |
OMAi | MMVLWER |
PhylomeDBi | O34777 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000835, HTH_MarR-typ IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01047, MarR, 1 hit |
PRINTSi | PR00598, HTHMARR |
SMARTi | View protein in SMART SM00347, HTH_MARR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50995, HTH_MARR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O34777-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MENKFDHMKL ENQLCFLLYA SSREMTKQYK PLLDKLNITY PQYLALLLLW
60 70 80 90 100
EHETLTVKKM GEQLYLDSGT LTPMLKRMEQ QGLITRKRSE EDERSVLISL
110 120 130 140
TEDGALLKEK AVDIPGTILG LSKQSGEDLK QLKSALYTLL ETLHQKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05594.1 AL009126 Genomic DNA Translation: CAB13172.1 |
PIRi | E69857 |
RefSeqi | NP_389198.1, NC_000964.3 WP_003245653.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13172; CAB13172; BSU_13150 |
GeneIDi | 939850 |
KEGGi | bsu:BSU13150 |
PATRICi | fig|224308.179.peg.1427 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05594.1 AL009126 Genomic DNA Translation: CAB13172.1 |
PIRi | E69857 |
RefSeqi | NP_389198.1, NC_000964.3 WP_003245653.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z91 | X-ray | 2.50 | A | 1-147 | [»] | |
1Z9C | X-ray | 2.64 | A/B/C/D/E/F | 1-147 | [»] | |
AlphaFoldDBi | O34777 | |||||
SMRi | O34777 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU13150 |
Proteomic databases
PaxDbi | O34777 |
PRIDEi | O34777 |
Genome annotation databases
EnsemblBacteriai | CAB13172; CAB13172; BSU_13150 |
GeneIDi | 939850 |
KEGGi | bsu:BSU13150 |
PATRICi | fig|224308.179.peg.1427 |
Phylogenomic databases
eggNOGi | COG1846, Bacteria |
InParanoidi | O34777 |
OMAi | MMVLWER |
PhylomeDBi | O34777 |
Enzyme and pathway databases
BioCyci | BSUB:BSU13150-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O34777 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000835, HTH_MarR-typ IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01047, MarR, 1 hit |
PRINTSi | PR00598, HTHMARR |
SMARTi | View protein in SMART SM00347, HTH_MARR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50995, HTH_MARR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OHRR_BACSU | |
Accessioni | O34777Primary (citable) accession number: O34777 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references