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UniProtKB - O34714 (OXDC_BACSU)
Protein
Oxalate decarboxylase OxdC
Gene
oxdC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Converts oxalate to formate and CO2 in an O2-dependent reaction. Can also catalyze minor side reactions: oxalate oxidation to produce H2O2, and oxalate-dependent, H2O2-independent dye oxidations.
3 PublicationsCatalytic activityi
- EC:4.1.1.23 Publications
Cofactori
Mn2+2 PublicationsNote: Binds 2 manganese ions per subunit.1 Publication
Kineticsi
- KM=15 mM for oxalate1 Publication
- Vmax=75 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 5.0.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 95 | Manganese 11 Publication | 1 | |
Metal bindingi | 97 | Manganese 11 Publication | 1 | |
Metal bindingi | 101 | Manganese 11 Publication | 1 | |
Metal bindingi | 140 | Manganese 11 Publication | 1 | |
Metal bindingi | 273 | Manganese 21 Publication | 1 | |
Metal bindingi | 275 | Manganese 21 Publication | 1 | |
Metal bindingi | 280 | Manganese 21 Publication | 1 | |
Metal bindingi | 319 | Manganese 21 Publication | 1 | |
Active sitei | 333 | Proton donor1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- oxalate decarboxylase activity Source: UniProtKB-EC
GO - Biological processi
- oxalate metabolic process Source: InterPro
Keywordsi
Molecular function | Decarboxylase, Lyase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU33240-MONOMER |
BRENDAi | 4.1.1.2, 658 |
SABIO-RKi | O34714 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:oxdC1 Publication Synonyms:yvrK1 Publication Ordered Locus Names:BSU33240 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 270 | R → E: Leads to a 20-fold reduction of CO(2) production. 1 Publication | 1 | |
Mutagenesisi | 333 | E → A: Leads to a 25-fold reduction of activity and a 4-fold reduction of CO(2) production. 1 Publication | 1 | |
Mutagenesisi | 340 | Y → F: Leads to a 13-fold reduction of CO(2) production. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB01942, Formic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058106 | 1 – 385 | Oxalate decarboxylase OxdCAdd BLAST | 385 |
Proteomic databases
jPOSTi | O34714 |
PaxDbi | O34714 |
PRIDEi | O34714 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homohexamer.
2 PublicationsProtein-protein interaction databases
STRINGi | 224308.BSU33240 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O34714 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O34714 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 192 | Cupin type-1 1Sequence analysisAdd BLAST | 143 | |
Domaini | 228 – 369 | Cupin type-1 2Sequence analysisAdd BLAST | 142 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 47 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
To B.subtilis OxdD.Curated
Phylogenomic databases
eggNOGi | COG2140, Bacteria |
InParanoidi | O34714 |
OMAi | TRQTTIR |
PhylomeDBi | O34714 |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR017774, Bicupin_oxalate_deCO2ase/Oxase IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 2 hits |
SMARTi | View protein in SMART SM00835, Cupin_1, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
TIGRFAMsi | TIGR03404, bicupin_oxalic, 1 hit |
i Sequence
Sequence statusi: Complete.
O34714-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKQNDIPQP IRGDKGATVK IPRNIERDRQ NPDMLVPPET DHGTVSNMKF
60 70 80 90 100
SFSDTHNRLE KGGYAREVTV RELPISENLA SVNMRLKPGA IRELHWHKEA
110 120 130 140 150
EWAYMIYGSA RVTIVDEKGR SFIDDVGEGD LWYFPSGLPH SIQALEEGAE
160 170 180 190 200
FLLVFDDGSF SENSTFQLTD WLAHTPKEVI AANFGVTKEE ISNLPGKEKY
210 220 230 240 250
IFENQLPGSL KDDIVEGPNG EVPYPFTYRL LEQEPIESEG GKVYIADSTN
260 270 280 290 300
FKVSKTIASA LVTVEPGAMR ELHWHPNTHE WQYYISGKAR MTVFASDGHA
310 320 330 340 350
RTFNYQAGDV GYVPFAMGHY VENIGDEPLV FLEIFKDDHY ADVSLNQWLA
360 370 380
MLPETFVQAH LDLGKDFTDV LSKEKHPVVK KKCSK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223978 Genomic DNA Translation: CAA11727.1 AL009126 Genomic DNA Translation: CAB15314.1 |
PIRi | E70047 |
RefSeqi | NP_391204.1, NC_000964.3 WP_003243476.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15314; CAB15314; BSU_33240 |
GeneIDi | 938620 |
KEGGi | bsu:BSU33240 |
PATRICi | fig|224308.179.peg.3608 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223978 Genomic DNA Translation: CAA11727.1 AL009126 Genomic DNA Translation: CAB15314.1 |
PIRi | E70047 |
RefSeqi | NP_391204.1, NC_000964.3 WP_003243476.1, NZ_JNCM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J58 | X-ray | 1.75 | A | 1-385 | [»] | |
1L3J | X-ray | 1.90 | A | 1-385 | [»] | |
1UW8 | X-ray | 2.00 | A | 1-385 | [»] | |
2UY8 | X-ray | 2.80 | A | 1-385 | [»] | |
2UY9 | X-ray | 3.10 | A | 1-385 | [»] | |
2UYA | X-ray | 2.00 | A | 1-385 | [»] | |
2UYB | X-ray | 2.10 | A | 1-385 | [»] | |
2V09 | X-ray | 1.80 | A | 1-385 | [»] | |
3S0M | X-ray | 2.31 | A | 6-382 | [»] | |
4MET | X-ray | 2.10 | A/B/C/D | 1-382 | [»] | |
5HI0 | X-ray | 2.60 | A | 1-385 | [»] | |
5VG3 | X-ray | 1.45 | A/B/C | 1-382 | [»] | |
6UFI | X-ray | 1.72 | A | 1-385 | [»] | |
SMRi | O34714 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU33240 |
Chemistry databases
DrugBanki | DB01942, Formic acid |
Proteomic databases
jPOSTi | O34714 |
PaxDbi | O34714 |
PRIDEi | O34714 |
Genome annotation databases
EnsemblBacteriai | CAB15314; CAB15314; BSU_33240 |
GeneIDi | 938620 |
KEGGi | bsu:BSU33240 |
PATRICi | fig|224308.179.peg.3608 |
Phylogenomic databases
eggNOGi | COG2140, Bacteria |
InParanoidi | O34714 |
OMAi | TRQTTIR |
PhylomeDBi | O34714 |
Enzyme and pathway databases
BioCyci | BSUB:BSU33240-MONOMER |
BRENDAi | 4.1.1.2, 658 |
SABIO-RKi | O34714 |
Miscellaneous databases
EvolutionaryTracei | O34714 |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR017774, Bicupin_oxalate_deCO2ase/Oxase IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 2 hits |
SMARTi | View protein in SMART SM00835, Cupin_1, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
TIGRFAMsi | TIGR03404, bicupin_oxalic, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OXDC_BACSU | |
Accessioni | O34714Primary (citable) accession number: O34714 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 6, 2002 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references