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Protein

Probable BsuMI modification methylase subunit YdiP

Gene

ydiP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This methylase may recognize the double-stranded sequence CTCGAG, causing specific methylation on C-? on both strands, and protects the DNA from cleavage by the BsuMI endonuclease.2 Publications

Caution

The characterized enzyme was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69PROSITE-ProRule annotation1

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  • methyltransferase activity Source: UniProtKB

GO - Biological processi

  • DNA methylation on cytosine Source: UniProtKB
  • DNA restriction-modification system Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU06070-MONOMER

Protein family/group databases

REBASEi3613 M2.BsuMI

Names & Taxonomyi

Protein namesi
Recommended name:
Probable BsuMI modification methylase subunit YdiP (EC:2.1.1.37)
Short name:
M2.BsuMI
Alternative name(s):
Cytosine-specific methyltransferase M2.BsuMI
Gene namesi
Name:ydiP
Ordered Locus Names:BSU06070
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025) has been disrupted.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003798851 – 389Probable BsuMI modification methylase subunit YdiPAdd BLAST389

Proteomic databases

PaxDbiO34680
PRIDEiO34680

Expressioni

Developmental stagei

Not expressed during sporulation.1 Publication

Inductioni

Constitutively expressed during exponential growth. Encoded in an operon with ydiO and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible.1 Publication

Interactioni

Subunit structurei

May form a complex with YdiO, also seems to be active alone.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003408

Structurei

3D structure databases

ProteinModelPortaliO34680
SMRiO34680
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 299SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST299

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107RHI Bacteria
COG0270 LUCA
HOGENOMiHOG000226162
InParanoidiO34680
KOiK00558
OMAiGWAVNAV
PhylomeDBiO34680

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 2 hits
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

O34680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVVSLFSGI GGIELGLHQS GHTTEIFCEV DPLAKAVLSK NFPGVKIEDD
60 70 80 90 100
INEIRELPSC DLVAAGFPCQ DLSQAGGKEG IDGSRSGLVK KLFELIEKKE
110 120 130 140 150
HANRPPWILI ENVPYMLRLN RGKAMSYLTS VLSELGYTWA YRTVDARCFG
160 170 180 190 200
LPQRRHRVIL LASLFEDPKD VIFSQDHSEP DLDGKPSVVD HSNYYGFYWT
210 220 230 240 250
EGLRGVGWAR EAVPPIKCGS SVGIASPPAV WSPYEDIVGT INIRDAERLQ
260 270 280 290 300
GFPEDWTNIT TETGKDIKEG ARWRLVGNAV SVRVSKWIGE NLSQPKGSIS
310 320 330 340 350
DFEGELVTKT WPSAAWGYGD KKYKVPVSKW VANTEQIAIS EFLNHPLKPL
360 370 380
SARALNGFLG RAARCTNVNY SDEFINSLER CKDRQLQKV
Length:389
Mass (Da):43,284
Last modified:January 1, 1998 - v1
Checksum:i16578833225AFE22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007637 Genomic DNA Translation: BAA22751.1
AL009126 Genomic DNA Translation: CAB12426.1
PIRiA69788
RefSeqiNP_388488.1, NC_000964.3
WP_003242904.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12426; CAB12426; BSU06070
GeneIDi939876
KEGGibsu:BSU06070
PATRICifig|224308.43.peg.637

Similar proteinsi

Entry informationi

Entry nameiYDIP_BACSU
AccessioniPrimary (citable) accession number: O34680
Secondary accession number(s): Q797D0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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