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Protein

Thiamine pyrophosphokinase

Gene

thiN

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate.

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Kineticsi

  1. KM=20 µM for thiamine
  2. KM=1 mM for ATP

    Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Thiamine pyrophosphokinase (thiN)
    This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU15800-MONOMER
    MetaCyc:BSU15800-MONOMER
    UniPathwayiUPA00060; UER00597

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine pyrophosphokinase (EC:2.7.6.2)
    Short name:
    TPK
    Alternative name(s):
    Thiamine diphosphokinase
    Gene namesi
    Name:thiN
    Synonyms:yloS
    Ordered Locus Names:BSU15800
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000725161 – 214Thiamine pyrophosphokinaseAdd BLAST214

    Proteomic databases

    PaxDbiO34664
    PRIDEiO34664

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100008721

    Structurei

    Secondary structure

    1214
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 7Combined sources5
    Helixi12 – 14Combined sources3
    Helixi19 – 21Combined sources3
    Beta strandi26 – 31Combined sources6
    Helixi33 – 41Combined sources9
    Beta strandi46 – 50Combined sources5
    Helixi57 – 66Combined sources10
    Beta strandi71 – 73Combined sources3
    Beta strandi77 – 79Combined sources3
    Helixi81 – 92Combined sources12
    Beta strandi95 – 101Combined sources7
    Helixi107 – 122Combined sources16
    Beta strandi126 – 131Combined sources6
    Beta strandi134 – 139Combined sources6
    Beta strandi141 – 147Combined sources7
    Beta strandi154 – 159Combined sources6
    Beta strandi164 – 174Combined sources11
    Beta strandi176 – 181Combined sources6
    Beta strandi189 – 191Combined sources3
    Beta strandi193 – 205Combined sources13
    Beta strandi207 – 212Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3LM8X-ray2.60A/B/C/D1-214[»]
    ProteinModelPortaliO34664
    SMRiO34664
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO34664

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiamine pyrophosphokinase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105FJ0 Bacteria
    COG1564 LUCA
    HOGENOMiHOG000037072
    InParanoidiO34664
    KOiK00949
    OMAiTGFKYPL
    PhylomeDBiO34664

    Family and domain databases

    CDDicd07995 TPK, 1 hit
    Gene3Di3.40.50.10240, 1 hit
    InterProiView protein in InterPro
    IPR006282 Thi_PPkinase
    IPR007373 Thiamin_PyroPKinase_B1-bd
    IPR036371 TPK_B1-bd_sf
    IPR007371 TPK_catalytic
    IPR036759 TPK_catalytic_sf
    PfamiView protein in Pfam
    PF04265 TPK_B1_binding, 1 hit
    PF04263 TPK_catalytic, 1 hit
    SMARTiView protein in SMART
    SM00983 TPK_B1_binding, 1 hit
    SUPFAMiSSF63862 SSF63862, 1 hit
    SSF63999 SSF63999, 1 hit
    TIGRFAMsiTIGR01378 thi_PPkinase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O34664-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTINIVAGG PKNLIPDLTG YTDEHTLWIG VDKGTVTLLD AGIIPVEAFG
    60 70 80 90 100
    DFDSITEQER RRIEKAAPAL HVYQAEKDQT DLDLALDWAL EKQPDIIQIF
    110 120 130 140 150
    GITGGRADHF LGNIQLLYKG VKTNIKIRLI DKQNHIQMFP PGEYDIEKDE
    160 170 180 190 200
    NKRYISFIPF SEDIHELTLT GFKYPLNNCH ITLGSTLCIS NELIHSRGTF
    210
    SFAKGILIMI RSTD
    Length:214
    Mass (Da):24,099
    Last modified:January 1, 1998 - v1
    Checksum:i77BBA150330F4679
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y13937 Genomic DNA Translation: CAA74253.1
    AL009126 Genomic DNA Translation: CAB13453.1
    PIRiC69879
    RefSeqiNP_389462.1, NC_000964.3
    WP_003245470.1, NZ_JNCM01000035.1

    Genome annotation databases

    EnsemblBacteriaiCAB13453; CAB13453; BSU15800
    GeneIDi937714
    KEGGibsu:BSU15800
    PATRICifig|224308.179.peg.1720

    Similar proteinsi

    Entry informationi

    Entry nameiTHIN_BACSU
    AccessioniPrimary (citable) accession number: O34664
    Secondary accession number(s): Q799K8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: January 1, 1998
    Last modified: May 23, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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