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UniProtKB - O34580 (PCRA_BACSU)
Protein
ATP-dependent DNA helicase PcrA
Gene
pcrA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
DNA helicase used for plasmid rolling-circle replication and also involved in UV repair.
Catalytic activityi
- EC:3.6.4.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 287 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 39 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA unwinding involved in DNA replication Source: InterPro
- recombinational repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU06610-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pcrA Synonyms:yerF Ordered Locus Names:BSU06610 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- DNA helicase complex Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102051 | 1 – 739 | ATP-dependent DNA helicase PcrAAdd BLAST | 739 |
Proteomic databases
PaxDbi | O34580 |
Interactioni
Subunit structurei
Interacts with YxaL; YwhK and YerB.
Interacts with the RNA polymerase core (PubMed:21710567).
2 PublicationsProtein-protein interaction databases
IntActi | O34580, 5 interactors |
STRINGi | 224308.BSU06610 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 289 | UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 280 | |
Domaini | 290 – 570 | UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 281 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 655 – 678 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
InParanoidi | O34580 |
OMAi | YQDTNRT |
PhylomeDBi | O34580 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR005751, ATP-dep_DNA_helicase_PcrA IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01073, pcrA, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
O34580-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNYISNQLLS GLNPVQQEAV KTTDGPLLLM AGAGSGKTRV LTHRIAYLMA
60 70 80 90 100
EKHVAPWNIL AITFTNKAAR EMKERVESIL GPGADDIWIS TFHSMCVRIL
110 120 130 140 150
RRDIDRIGIN RNFSILDTAD QLSVIKGILK ERNLDPKKFD PRSILGTISS
160 170 180 190 200
AKNELTEPEE FSKVAGGYYD QVVSDVYADY QKKLLKNQSL DFDDLIMTTI
210 220 230 240 250
KLFDRVPEVL EFYQRKFQYI HVDEYQDTNR AQYMLVKQLA ERFQNLCVVG
260 270 280 290 300
DSDQSIYRWR GADITNILSF EKDYPNASVI LLEQNYRSTK RILRAANEVI
310 320 330 340 350
KNNSNRKPKN LWTENDEGIK ISYYRGDNEF GEGQFVAGKI HQLHSTGKRK
360 370 380 390 400
LSDIAILYRT NAQSRVIEET LLKAGLNYNI VGGTKFYDRK EIKDILAYLR
410 420 430 440 450
LVSNPDDDIS FTRIVNVPKR GVGATSLEKI ASYAAINGLS FFQAIQQVDF
460 470 480 490 500
IGVSAKAANA LDSFRQMIEN LTNMQDYLSI TELTEEILDK TEYREMLKAE
510 520 530 540 550
KSIEAQSRLE NIDEFLSVTK NFEQKSEDKT LVAFLTDLAL IADIDQLDQK
560 570 580 590 600
EEESGGKDAI TLMTLHAAKG LEFPVVFLMG LEEGVFPHSR SLMEEAEMEE
610 620 630 640 650
ERRLAYVGIT RAEQELYLTN AKMRTLFGRT NMNPESRFIA EIPDDLLENL
660 670 680 690 700
NEKKETRATS ARKMQPRRGP VSRPVSYASK TGGDTLNWAV GDKAGHKKWG
710 720 730
TGTVVSVKGE GEGTELDIAF PSPVGVKRLL AAFAPIEKQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y15254 Genomic DNA Translation: CAA75552.1 AL009126 Genomic DNA Translation: CAB12481.1 |
PIRi | E69794 |
RefSeqi | NP_388543.1, NC_000964.3 WP_003233919.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12481; CAB12481; BSU_06610 |
GeneIDi | 938747 |
KEGGi | bsu:BSU06610 |
PATRICi | fig|224308.179.peg.719 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y15254 Genomic DNA Translation: CAA75552.1 AL009126 Genomic DNA Translation: CAB12481.1 |
PIRi | E69794 |
RefSeqi | NP_388543.1, NC_000964.3 WP_003233919.1, NZ_JNCM01000032.1 |
3D structure databases
SMRi | O34580 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O34580, 5 interactors |
STRINGi | 224308.BSU06610 |
Proteomic databases
PaxDbi | O34580 |
Genome annotation databases
EnsemblBacteriai | CAB12481; CAB12481; BSU_06610 |
GeneIDi | 938747 |
KEGGi | bsu:BSU06610 |
PATRICi | fig|224308.179.peg.719 |
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
InParanoidi | O34580 |
OMAi | YQDTNRT |
PhylomeDBi | O34580 |
Enzyme and pathway databases
BioCyci | BSUB:BSU06610-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR005751, ATP-dep_DNA_helicase_PcrA IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01073, pcrA, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCRA_BACSU | |
Accessioni | O34580Primary (citable) accession number: O34580 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families