UniProtKB - O34530 (RSGA_BACSU)
Small ribosomal subunit biogenesis GTPase RsgA
rsgA
Functioni
One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP (PubMed:16485133).
GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation (PubMed:22544754).
Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo (PubMed:15828870).
Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes (PubMed:15828870).
Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis (PubMed:18344364).
4 PublicationsCofactori
Kineticsi
- KM=30.5 µM for GTP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 252 | ZincUniRule annotation1 Publication | 1 | |
Metal bindingi | 257 | ZincUniRule annotation1 Publication | 1 | |
Metal bindingi | 259 | Zinc; via pros nitrogenUniRule annotation1 Publication | 1 | |
Metal bindingi | 265 | ZincUniRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 116 – 119 | GTPUniRule annotation | 4 | |
Nucleotide bindingi | 171 – 179 | GTPUniRule annotation | 9 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB-UniRule
- GTP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- rRNA binding Source: UniProtKB-KW
GO - Biological processi
- ribosomal small subunit biogenesis Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase, RNA-binding, rRNA-binding |
Biological process | Ribosome biogenesis |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU15780-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Small ribosomal subunit biogenesis GTPase RsgAUniRule annotation (EC:3.6.1.-UniRule annotation1 Publication)Alternative name(s): Ribosome-associated GTPase CpgA1 Publication |
Gene namesi | Name:rsgAUniRule annotation Synonyms:cpgA1 Publication, engC, yloQ1 Publication Ordered Locus Names:BSU15780 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 166 | T → A: Loss of most phosphorylation by PrkC, GTPase activity less stimulated by 70S ribosomes than wild-type, decreased association with 30S ribosomal subunit in vitro. Grows slowly, cells have abnormal morphology. 1 Publication | 1 | |
Mutagenesisi | 166 | T → D: Higher endogenous GTPase activity, GTPase more stimulated by 70S ribosomes than wild-type, increased association with 30S ribosomal subunit in vitro, wild-type behavior in vivo. 1 Publication | 1 | |
Mutagenesisi | 177 | K → A: Grows slowly, cells have abnormal morphology. 1 Publication | 1 | |
Mutagenesisi | 178 | S → A: No GTPase activity. Wild-type phosphorylation by PrkC in vitro. 1 Publication | 1 | |
Mutagenesisi | 192 | T → A: Loss of phosphorylation by PrkC, but PrkC autophosphorylation greatly decreased in vitro. 1 Publication | 1 | |
Mutagenesisi | 196 | S → A: Wild-type phosphorylation by PrkC in vitro. 1 Publication | 1 | |
Mutagenesisi | 206 | T → A: Wild-type phosphorylation by PrkC in vitro. 1 Publication | 1 | |
Mutagenesisi | 222 | T → A: Wild-type phosphorylation by PrkC in vitro. 1 Publication | 1 | |
Mutagenesisi | 226 | S → A: Loss of phosphorylation by PrkC, but PrkC autophosphorylation considerably decreased in vitro. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171464 | 1 – 298 | Small ribosomal subunit biogenesis GTPase RsgAAdd BLAST | 298 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 166 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O34530 |
PaxDbi | O34530 |
PRIDEi | O34530 |
PTM databases
iPTMneti | O34530 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O34530 |
SMRi | O34530 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O34530 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 67 – 228 | CP-type GPROSITE-ProRule annotationAdd BLAST | 162 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1162, Bacteria |
InParanoidi | O34530 |
OMAi | CLVAAYD |
PhylomeDBi | O34530 |
Family and domain databases
CDDi | cd04466, S1_YloQ_GTPase, 1 hit cd01854, YjeQ_EngC, 1 hit |
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01820, GTPase_RsgA, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR004881, Ribosome_biogen_GTPase_RsgA IPR010914, RsgA_GTPase_dom IPR031944, RsgA_N |
PANTHERi | PTHR32120, PTHR32120, 1 hit |
Pfami | View protein in Pfam PF03193, RsgA_GTPase, 1 hit PF16745, RsgA_N, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00157, TIGR00157, 1 hit |
PROSITEi | View protein in PROSITE PS50936, ENGC_GTPASE, 1 hit PS51721, G_CP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPEGKIIKAL SGFYYVLDES EDSDKVIQCR GRGIFRKNKI TPLVGDYVVY
60 70 80 90 100
QAENDKEGYL MEIKERTNEL IRPPICNVDQ AVLVFSAVQP SFSTALLDRF
110 120 130 140 150
LVLVEANDIQ PIICITKMDL IEDQDTEDTI QAYAEDYRNI GYDVYLTSSK
160 170 180 190 200
DQDSLADIIP HFQDKTTVFA GQSGVGKSSL LNAISPELGL RTNEISEHLG
210 220 230 240 250
RGKHTTRHVE LIHTSGGLVA DTPGFSSLEF TDIEEEELGY TFPDIREKSS
260 270 280 290
SCKFRGCLHL KEPKCAVKQA VEDGELKQYR YDHYVEFMTE IKDRKPRY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13937 Genomic DNA Translation: CAA74251.1 AL009126 Genomic DNA Translation: CAB13451.1 |
PIRi | A69879 |
RefSeqi | NP_389460.1, NC_000964.3 WP_003232060.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13451; CAB13451; BSU_15780 |
GeneIDi | 938451 |
KEGGi | bsu:BSU15780 |
PATRICi | fig|224308.179.peg.1718 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13937 Genomic DNA Translation: CAA74251.1 AL009126 Genomic DNA Translation: CAB13451.1 |
PIRi | A69879 |
RefSeqi | NP_389460.1, NC_000964.3 WP_003232060.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T9H | X-ray | 1.60 | A | 1-298 | [»] | |
AlphaFoldDBi | O34530 | |||||
SMRi | O34530 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU15780 |
PTM databases
iPTMneti | O34530 |
Proteomic databases
jPOSTi | O34530 |
PaxDbi | O34530 |
PRIDEi | O34530 |
Genome annotation databases
EnsemblBacteriai | CAB13451; CAB13451; BSU_15780 |
GeneIDi | 938451 |
KEGGi | bsu:BSU15780 |
PATRICi | fig|224308.179.peg.1718 |
Phylogenomic databases
eggNOGi | COG1162, Bacteria |
InParanoidi | O34530 |
OMAi | CLVAAYD |
PhylomeDBi | O34530 |
Enzyme and pathway databases
BioCyci | BSUB:BSU15780-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O34530 |
Family and domain databases
CDDi | cd04466, S1_YloQ_GTPase, 1 hit cd01854, YjeQ_EngC, 1 hit |
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01820, GTPase_RsgA, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR004881, Ribosome_biogen_GTPase_RsgA IPR010914, RsgA_GTPase_dom IPR031944, RsgA_N |
PANTHERi | PTHR32120, PTHR32120, 1 hit |
Pfami | View protein in Pfam PF03193, RsgA_GTPase, 1 hit PF16745, RsgA_N, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00157, TIGR00157, 1 hit |
PROSITEi | View protein in PROSITE PS50936, ENGC_GTPASE, 1 hit PS51721, G_CP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RSGA_BACSU | |
Accessioni | O34530Primary (citable) accession number: O34530 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2003 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families