UniProtKB - O34525 (SPPA_BACSU)
Protein
Putative signal peptide peptidase SppA
Gene
sppA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Digestion of cleaved signal peptides (By similarity). Required for efficient processing of precursors under conditions of hyper-secretion. Has a preference for leucine-rich substrate peptides.By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 147 | Nucleophile1 Publication | 1 | |
Active sitei | 199 | Proton donor/acceptor1 Publication | 1 |
GO - Molecular functioni
- serine-type peptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | BSUB:BSU29530-MONOMER |
Protein family/group databases
MEROPSi | S49.008 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:sppA Synonyms:yteI Ordered Locus Names:BSU29530 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Single-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 147 | S → A: Abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 199 | K → A: Abolishes enzyme activity. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171442 | 1 – 335 | Putative signal peptide peptidase SppAAdd BLAST | 335 |
Post-translational modificationi
Autocatalytically cleaves its own C-terminus.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 294 – 295 | Cleavage; by autolysis | 2 |
Proteomic databases
jPOSTi | O34525 |
PaxDbi | O34525 |
Interactioni
Subunit structurei
Homooctamer, assembles into a ring structure with a central cavity.
2 PublicationsProtein-protein interaction databases
STRINGi | 224308.BSU29530 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O34525 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S49 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0616, Bacteria |
InParanoidi | O34525 |
OMAi | CDELWAR |
PhylomeDBi | O34525 |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00706, SppA_dom, 1 hit |
i Sequence
Sequence statusi: Complete.
O34525-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNAKRWIALV IALGIFGVSI IVSISMSFFE SVKGAQTDLT SLTDESQEKT
60 70 80 90 100
LENGSPSSKI AVLEVSGTIQ DNGDSSSLLG ADGYNHRTFL KNLERAKDDK
110 120 130 140 150
TVKGIVLKVN SPGGGVYESA EIHKKLEEIK KETKKPIYVS MGSMAASGGY
160 170 180 190 200
YISTAADKIF ATPETLTGSL GVIMESVNYS KLADKLGISF ETIKSGAHKD
210 220 230 240 250
IMSPSREMTK EEKNIMQSMV DNSYEGFVDV ISKGRGMPKA EVKKIADGRV
260 270 280 290 300
YDGRQAKKLN LVDELGFYDD TITAMKKDHK DLKNASVISY EESFGLGSLF
310 320 330
SMGANKMFKS EIDFLNMREI LSQSGSPRMM YLYAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00312.1 AL009126 Genomic DNA Translation: CAB14931.1 |
PIRi | E69990 |
RefSeqi | NP_390831.1, NC_000964.3 WP_003229337.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14931; CAB14931; BSU29530 |
GeneIDi | 937679 |
KEGGi | bsu:BSU29530 |
PATRICi | fig|224308.179.peg.3208 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00312.1 AL009126 Genomic DNA Translation: CAB14931.1 |
PIRi | E69990 |
RefSeqi | NP_390831.1, NC_000964.3 WP_003229337.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3RST | X-ray | 2.37 | A/B/C/D/E/F/G/H | 56-295 | [»] | |
4KWB | X-ray | 2.39 | A/B/C/D/E/F/G/H | 51-335 | [»] | |
SMRi | O34525 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU29530 |
Protein family/group databases
MEROPSi | S49.008 |
Proteomic databases
jPOSTi | O34525 |
PaxDbi | O34525 |
Protocols and materials databases
DNASUi | 937679 |
Genome annotation databases
EnsemblBacteriai | CAB14931; CAB14931; BSU29530 |
GeneIDi | 937679 |
KEGGi | bsu:BSU29530 |
PATRICi | fig|224308.179.peg.3208 |
Phylogenomic databases
eggNOGi | COG0616, Bacteria |
InParanoidi | O34525 |
OMAi | CDELWAR |
PhylomeDBi | O34525 |
Enzyme and pathway databases
BioCyci | BSUB:BSU29530-MONOMER |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR004635, Pept_S49_SppA IPR002142, Peptidase_S49 |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00706, SppA_dom, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPPA_BACSU | |
Accessioni | O34525Primary (citable) accession number: O34525 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families