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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

gapB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NADP. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NADP to NADPH. The reduced NADPH is then exchanged with the second NADP, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.1 Publication

Kineticsi

Kcat is 1 sec(-1) for dehydrogenase activity with NAD (at pH 9.2 and 25 degrees Celsius). Kcat is 8 sec(-1) for dehydrogenase activity with NADP (at pH 9.2 and 25 degrees Celsius).1 Publication
  1. KM=0.86 mM for NADP (at pH 9.2 and 25 degrees Celsius)1 Publication
  2. KM=5.7 mM for NAD (at pH 9.2 and 25 degrees Celsius)1 Publication

    Pathwayi: gluconeogenesis

    This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei78NADP; via carbonyl oxygenBy similarity1
    Binding sitei120NADPBy similarity1
    Active sitei152NucleophileBy similarity1
    Sitei179Activates thiol group during catalysisBy similarity1
    Binding sitei182Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei183NADPBy similarity1
    Binding sitei197Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei233Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei315NADPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 13NADPBy similarity2

    GO - Molecular functioni

    GO - Biological processi

    • gluconeogenesis Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processGluconeogenesis
    LigandNAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU29020-MONOMER
    BRENDAi1.2.1.59 658
    SABIO-RKiO34425
    UniPathwayi
    UPA00138

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 21 Publication (EC:1.2.1.591 Publication)
    Short name:
    GAPDH1 Publication
    Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase1 Publication
    Gene namesi
    Name:gapB1 Publication
    Ordered Locus Names:BSU29020
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are unable to grow in medium containing asparagine as a sole carbon source but presents the same growth rate as the wild-type in glucose-containing medium.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001456351 – 340Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST340

    Proteomic databases

    PaxDbiO34425
    PRIDEiO34425

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100015831

    Structurei

    3D structure databases

    ProteinModelPortaliO34425
    SMRiO34425
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni151 – 153Glyceraldehyde 3-phosphate bindingBy similarity3
    Regioni210 – 211Glyceraldehyde 3-phosphate bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C17 Bacteria
    COG0057 LUCA
    HOGENOMiHOG000071679
    InParanoidiO34425
    KOiK00134
    OMAiNAKVLAW
    PhylomeDBiO34425

    Family and domain databases

    InterProiView protein in InterPro
    IPR020831 GlycerAld/Erythrose_P_DH
    IPR020830 GlycerAld_3-P_DH_AS
    IPR020829 GlycerAld_3-P_DH_cat
    IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
    IPR006424 Glyceraldehyde-3-P_DH_1
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF02800 Gp_dh_C, 1 hit
    PF00044 Gp_dh_N, 1 hit
    PIRSFiPIRSF000149 GAP_DH, 1 hit
    PRINTSiPR00078 G3PDHDRGNASE
    SMARTiView protein in SMART
    SM00846 Gp_dh_N, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
    PROSITEiView protein in PROSITE
    PS00071 GAPDH, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O34425-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKVKVAINGF GRIGRMVFRK AMLDDQIQVV AINASYSAET LAHLIKYDTI
    60 70 80 90 100
    HGRYDKEVVA GEDSLIVNGK KVLLLNSRDP KQLPWREYDI DIVVEATGKF
    110 120 130 140 150
    NAKDKAMGHI EAGAKKVILT APGKNEDVTI VMGVNEDQFD AERHVIISNA
    160 170 180 190 200
    SCTTNCLAPV VKVLDEEFGI ESGLMTTVHA YTNDQKNIDN PHKDLRRARA
    210 220 230 240 250
    CGESIIPTTT GAAKALSLVL PHLKGKLHGL ALRVPVPNVS LVDLVVDLKT
    260 270 280 290 300
    DVTAEEVNEA FKRAAKTSMY GVLDYSDEPL VSTDYNTNPH SAVIDGLTTM
    310 320 330 340
    VMEDRKVKVL AWYDNEWGYS CRVVDLIRHV AARMKHPSAV
    Length:340
    Mass (Da):37,476
    Last modified:January 1, 1998 - v1
    Checksum:iDD1DD4BC7D633EC6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF008220 Genomic DNA Translation: AAC00355.1
    AL009126 Genomic DNA Translation: CAB14862.1
    PIRiG69628
    RefSeqiNP_390780.1, NC_000964.3
    WP_003229459.1, NZ_JNCM01000036.1

    Genome annotation databases

    EnsemblBacteriaiCAB14862; CAB14862; BSU29020
    GeneIDi937393
    KEGGibsu:BSU29020
    PATRICifig|224308.179.peg.3151

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF008220 Genomic DNA Translation: AAC00355.1
    AL009126 Genomic DNA Translation: CAB14862.1
    PIRiG69628
    RefSeqiNP_390780.1, NC_000964.3
    WP_003229459.1, NZ_JNCM01000036.1

    3D structure databases

    ProteinModelPortaliO34425
    SMRiO34425
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100015831

    Proteomic databases

    PaxDbiO34425
    PRIDEiO34425

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB14862; CAB14862; BSU29020
    GeneIDi937393
    KEGGibsu:BSU29020
    PATRICifig|224308.179.peg.3151

    Phylogenomic databases

    eggNOGiENOG4105C17 Bacteria
    COG0057 LUCA
    HOGENOMiHOG000071679
    InParanoidiO34425
    KOiK00134
    OMAiNAKVLAW
    PhylomeDBiO34425

    Enzyme and pathway databases

    UniPathwayi
    UPA00138

    BioCyciBSUB:BSU29020-MONOMER
    BRENDAi1.2.1.59 658
    SABIO-RKiO34425

    Family and domain databases

    InterProiView protein in InterPro
    IPR020831 GlycerAld/Erythrose_P_DH
    IPR020830 GlycerAld_3-P_DH_AS
    IPR020829 GlycerAld_3-P_DH_cat
    IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
    IPR006424 Glyceraldehyde-3-P_DH_1
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF02800 Gp_dh_C, 1 hit
    PF00044 Gp_dh_N, 1 hit
    PIRSFiPIRSF000149 GAP_DH, 1 hit
    PRINTSiPR00078 G3PDHDRGNASE
    SMARTiView protein in SMART
    SM00846 Gp_dh_N, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
    PROSITEiView protein in PROSITE
    PS00071 GAPDH, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiG3P2_BACSU
    AccessioniPrimary (citable) accession number: O34425
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 25, 2017
    This is version 134 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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