UniProtKB - O34364 (O16G2_BACSU)
Protein
Probable oligo-1,6-glucosidase 2
Gene
ycdG
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose. EC:3.2.1.10
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 199 | NucleophileBy similarity | 1 | |
Active sitei | 255 | Proton donorBy similarity | 1 | |
Sitei | 330 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- alpha-amylase activity Source: GO_Central
- oligo-1,6-glucosidase activity Source: UniProtKB-EC
GO - Biological processi
- oligosaccharide catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | BSUB:BSU02840-MONOMER |
Protein family/group databases
CAZyi | GH13 Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable oligo-1,6-glucosidase 2 (EC:3.2.1.10)Alternative name(s): Oligosaccharide alpha-1,6-glucosidase 2 Sucrase-isomaltase 2 Short name: Isomaltase 2 |
Gene namesi | Name:ycdG Ordered Locus Names:BSU02840 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360818 | 1 – 561 | Probable oligo-1,6-glucosidase 2Add BLAST | 561 |
Proteomic databases
PaxDbi | O34364 |
PRIDEi | O34364 |
Expressioni
Inductioni
By ethanol, heat and salt via sigma B-dependent promoter.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Phylogenomic databases
eggNOGi | COG0366 LUCA |
HOGENOMi | HOG000220641 |
InParanoidi | O34364 |
KOi | K01182 |
OMAi | YLHMNAV |
PhylomeDBi | O34364 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128 Alpha-amylase, 1 hit |
SMARTi | View protein in SMART SM00642 Aamy, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
O34364-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTDWWKDAV VYQIYPRSFQ DSNGDGIGDL RGIISRLDYI KELGADVIWI
60 70 80 90 100
CPIYPSPNVD YGYDVTNHKA IMDSYGTMDD FHELLDQVHQ RGLKLVMDFV
110 120 130 140 150
LNHTSVEHPW FKEAELDKNS KYRSYYYWRP GTKNGPPTDW LSNYGCPVWQ
160 170 180 190 200
YEEHTGEYYL HMNAVKQADL NWENPEVRQA VYDMMKFWLD KGVDGLRIDQ
210 220 230 240 250
LHLISKKEYL PSYEDYINQQ AEPKPFQPNG ERIHDYLKEI TDEVFSHYDV
260 270 280 290 300
MSVGEVGSVT PEEGLKYTGT DKHELNMIFH FQHMELDQQP GKEHWDLKPL
310 320 330 340 350
ELSDLKSVLT KWQKKLEHQG WNTLFWCNHD QPRIVSRFGD DGEYRKASAK
360 370 380 390 400
MLAAVIYFMK GTPYIYQGEE IGMTNAPFTR IEDYKDIQTI NMYHKRVFEK
410 420 430 440 450
GYDPNDVMRS ILAKSRDHAR TPMQWNSGKN AGFTDGTPWL KVNPNFTAIN
460 470 480 490 500
VEEAQGDPDS VLNYYKKLIS LRKQYADLMK GSFDLLLPDD PQLFVYMREN
510 520 530 540 550
SKQQLLSVNN FSKEQAVFQW PKNCGKAQAS LLLSNYNNDD LDDEMVFRPY
560
ESRVYLLDKT N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000617 Genomic DNA Translation: BAA22245.1 AL009126 Genomic DNA Translation: CAB12078.1 |
PIRi | H69755 |
RefSeqi | NP_388166.1, NC_000964.3 WP_003234736.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12078; CAB12078; BSU02840 |
GeneIDi | 938375 |
KEGGi | bsu:BSU02840 |
PATRICi | fig|224308.179.peg.295 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000617 Genomic DNA Translation: BAA22245.1 AL009126 Genomic DNA Translation: CAB12078.1 |
PIRi | H69755 |
RefSeqi | NP_388166.1, NC_000964.3 WP_003234736.1, NZ_JNCM01000030.1 |
3D structure databases
SMRi | O34364 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU02840 |
Protein family/group databases
CAZyi | GH13 Glycoside Hydrolase Family 13 |
Proteomic databases
PaxDbi | O34364 |
PRIDEi | O34364 |
Genome annotation databases
EnsemblBacteriai | CAB12078; CAB12078; BSU02840 |
GeneIDi | 938375 |
KEGGi | bsu:BSU02840 |
PATRICi | fig|224308.179.peg.295 |
Phylogenomic databases
eggNOGi | COG0366 LUCA |
HOGENOMi | HOG000220641 |
InParanoidi | O34364 |
KOi | K01182 |
OMAi | YLHMNAV |
PhylomeDBi | O34364 |
Enzyme and pathway databases
BioCyci | BSUB:BSU02840-MONOMER |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128 Alpha-amylase, 1 hit |
SMARTi | View protein in SMART SM00642 Aamy, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | O16G2_BACSU | |
Accessioni | O34364Primary (citable) accession number: O34364 Secondary accession number(s): Q797R7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | October 16, 2019 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families - Bacillus subtilis
Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList