UniProtKB - O34358 (HTRA_BACSU)
Protein
Serine protease Do-like HtrA
Gene
htrA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Degrades abnormal exported proteins and responsible for the propeptide processing of a natural pro-protein and for the maturation of a native protein. It also plays a prominent role in stress (heat shock, ethanol, puromycin and NaCl) resistance during active exponential growth (Probable).1 Publication
Miscellaneous
Inactivation results in compensating overexpression of YtvA, especially during stress conditions.
Catalytic activityi
- Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val. EC:3.4.21.107
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 179 | Charge relay systemSequence analysis | 1 | |
Active sitei | 209 | Charge relay systemSequence analysis | 1 | |
Active sitei | 290 | Charge relay systemSequence analysis | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | BSUB:BSU12900-MONOMER |
Protein family/group databases
MEROPSi | S01.B81 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine protease Do-like HtrA (EC:3.4.21.107)Alternative name(s): HtrA-like serine protease |
Gene namesi | Name:htrA Synonyms:ykdA Ordered Locus Names:BSU12900 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 44 | CytoplasmicSequence analysisAdd BLAST | 44 | |
Transmembranei | 45 – 67 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 68 – 449 | ExtracellularSequence analysisAdd BLAST | 382 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
In contrast to other bacteria, in which inactivation of serine protease leads to thermosensitivity, inactivation of HtrA leads to an increased thermotolerance and an increased tolerance to hydrogen peroxide. Inactivation of both HtrA and HtrB leads to growth defects and to thermosensitivity.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093859 | 1 – 449 | Serine protease Do-like HtrAAdd BLAST | 449 |
Proteomic databases
PaxDbi | O34358 |
PRIDEi | O34358 |
Expressioni
Inductioni
Transcription is CssS dependent. Induced by heat shock during exponential growth and by heterologous amylases at the transition phase of the growth cycle. Negatively regulates its own expression during exponential growth and during heat shock.3 Publications
Interactioni
Protein-protein interaction databases
IntActi | O34358, 3 interactors |
STRINGi | 224308.BSU12900 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 348 – 437 | PDZPROSITE-ProRule annotationAdd BLAST | 90 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 288 – 290 | Substrate bindingBy similarity | 3 | |
Regioni | 344 – 348 | Substrate bindingBy similarity | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 103 – 108 | Poly-Ser | 6 | |
Compositional biasi | 146 – 152 | Poly-Ser | 7 |
Sequence similaritiesi
Belongs to the peptidase S1C family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0265, Bacteria |
InParanoidi | O34358 |
OMAi | CFCLHSS |
PhylomeDBi | O34358 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR001478, PDZ IPR036034, PDZ_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001940, Peptidase_S1C |
Pfami | View protein in Pfam PF13180, PDZ_2, 1 hit |
PRINTSi | PR00834, PROTEASES2C |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
i Sequence
Sequence statusi: Complete.
O34358-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNYRDENRT KGNENEVFLT KENDQSASYS ARNVIHDQEK KKRGFGWFRP
60 70 80 90 100
LLGGVIGGSL ALGIYTFTPL GDHDSQDTAK QSSSQQQTQS VTATSTSSES
110 120 130 140 150
KKSSSSSSAF KSEDSSKISD MVEDLSPAIV GITNLQAQSN SSLFGSSSSD
160 170 180 190 200
SSEDTESGSG SGVIFKKENG KAYIITNNHV VEGASSLKVS LYDGTEVTAK
210 220 230 240 250
LVGSDSLTDL AVLQISDDHV TKVANFGDSS DLRTGETVIA IGDPLGKDLS
260 270 280 290 300
RTVTQGIVSG VDRTVSMSTS AGETSINVIQ TDAAINPGNS GGPLLNTDGK
310 320 330 340 350
IVGINSMKIS EDDVEGIGFA IPSNDVKPIA EELLSKGQIE RPYIGVSMLD
360 370 380 390 400
LEQVPQNYQE GTLGLFGSQL NKGVYIREVA SGSPAEKAGL KAEDIIIGLK
410 420 430 440
GKEIDTGSEL RNILYKDAKI GDTVEVKILR NGKEMTKKIK LDQKEEKTS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 | D → N in CAA05570 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05570.1 AL009126 Genomic DNA Translation: CAB13147.2 |
PIRi | A69643 |
RefSeqi | NP_389173.2, NC_000964.3 WP_009967069.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13147; CAB13147; BSU_12900 |
GeneIDi | 939849 |
KEGGi | bsu:BSU12900 |
PATRICi | fig|224308.179.peg.1402 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002571 Genomic DNA Translation: CAA05570.1 AL009126 Genomic DNA Translation: CAB13147.2 |
PIRi | A69643 |
RefSeqi | NP_389173.2, NC_000964.3 WP_009967069.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | O34358 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O34358, 3 interactors |
STRINGi | 224308.BSU12900 |
Protein family/group databases
MEROPSi | S01.B81 |
Proteomic databases
PaxDbi | O34358 |
PRIDEi | O34358 |
Genome annotation databases
EnsemblBacteriai | CAB13147; CAB13147; BSU_12900 |
GeneIDi | 939849 |
KEGGi | bsu:BSU12900 |
PATRICi | fig|224308.179.peg.1402 |
Phylogenomic databases
eggNOGi | COG0265, Bacteria |
InParanoidi | O34358 |
OMAi | CFCLHSS |
PhylomeDBi | O34358 |
Enzyme and pathway databases
BioCyci | BSUB:BSU12900-MONOMER |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR001478, PDZ IPR036034, PDZ_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001940, Peptidase_S1C |
Pfami | View protein in Pfam PF13180, PDZ_2, 1 hit |
PRINTSi | PR00834, PROTEASES2C |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HTRA_BACSU | |
Accessioni | O34358Primary (citable) accession number: O34358 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families