UniProtKB - O34330 (YOBL_BACSU)
Protein
Ribonuclease YobL
Gene
yobL
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Toxic component of a type II toxin-antitoxin (TA) system. The C-terminus (residues 449-600) has RNase activity, and inhibits growth upon expression in E.coli. In vitro RNase activity and in vivo growth inhibition are neutralized by cognate antitoxin YobK, but not by antitoxins specific to other toxins with the LXG domain.1 Publication
GO - Molecular functioni
- nuclease activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Nuclease |
Biological process | Toxin-antitoxin system |
Enzyme and pathway databases
BioCyci | BSUB:BSU19000-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yobL Ordered Locus Names:BSU19000 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 562 | H → A: Loss of RNase activity, loss of E.coli growth inhibition. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360800 | 1 – 600 | Ribonuclease YobLAdd BLAST | 600 |
Proteomic databases
PaxDbi | O34330 |
PRIDEi | O34330 |
Interactioni
Subunit structurei
Interacts with cognate antitoxin YobK but not with non-cognate putative antitoxin YezG. The interaction inhibits the toxic activity of YobL.
1 PublicationProtein-protein interaction databases
IntActi | O34330, 1 interactor |
MINTi | O34330 |
STRINGi | 224308.BSU19000 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 235 | LXGPROSITE-ProRule annotationAdd BLAST | 235 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 12 – 40 | Sequence analysisAdd BLAST | 29 | |
Coiled coili | 141 – 176 | Sequence analysisAdd BLAST | 36 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | COG5444, Bacteria |
OMAi | MKNGNAP |
PhylomeDBi | O34330 |
Family and domain databases
InterProi | View protein in InterPro IPR026834, LHH IPR006829, LXG_dom |
Pfami | View protein in Pfam PF14411, LHH, 1 hit PF04740, LXG, 1 hit |
PROSITEi | View protein in PROSITE PS51756, LXG, 1 hit |
i Sequence
Sequence statusi: Complete.
O34330-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVFEADSLL FEADKRTKEY KELRSQMVKL KKAFKEVANL DDSEFSGKGA
60 70 80 90 100
DNIKAFYHGH VGVTDQWIDL IDMKIAFLSS MSATLEDAKM SDAYIEESFL
110 120 130 140 150
EHELANAYAK SKSIMSEQKK AMKDILNNIN DILPLEIFST EDFKDKLSSA
160 170 180 190 200
DDKREKTIDK LNKLDEDLKT EYAETEPNEQ FIQQDFKKLQ ESTGKGKNAT
210 220 230 240 250
PIHYNAKAYR ESDIHKKKGD IEKHSEAYLS VKKEEAKERE IKELKKKLND
260 270 280 290 300
GVSDPDEYLE IAKKVGYENL EPTQVQLAVQ IEQAKQLEGA GEITWDIVKG
310 320 330 340 350
VGVGLYDVGK DTVTGIWDFI TDPGETLSAL GNAAMHPVKT YDAISAAIEE
360 370 380 390 400
SYQKDMVNGD AYSRSRWVTY AIGSVAVAVV GTKGAGAINK ADAAGKVINK
410 420 430 440 450
ASQAGKKIKD VKIPDLLPYN PKYKLALADN VPYNVVDSQN LKNELLTNAK
460 470 480 490 500
KIPDGTRKPF TGQKKSPPWL NKEKYDAYEI EGKVKAKGKV KDVSRRVYTM
510 520 530 540 550
KDIDINQKTE FGVTNLQLMK NGNAPYAKDG TQINLHHLIQ EEPGPMLEIP
560 570 580 590 600
NSLHTKYSDV IHQLKSDGES FRNDKVLKAQ YESFRKRYWK WRAKQFENEN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027868 Genomic DNA Translation: AAB84464.1 AL009126 Genomic DNA Translation: CAB13792.1 |
PIRi | C69899 |
RefSeqi | NP_389781.1, NC_000964.3 WP_004399481.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB13792; CAB13792; BSU19000 |
GeneIDi | 939624 |
KEGGi | bsu:BSU19000 |
PATRICi | fig|224308.179.peg.2078 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027868 Genomic DNA Translation: AAB84464.1 AL009126 Genomic DNA Translation: CAB13792.1 |
PIRi | C69899 |
RefSeqi | NP_389781.1, NC_000964.3 WP_004399481.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | O34330 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O34330, 1 interactor |
MINTi | O34330 |
STRINGi | 224308.BSU19000 |
Proteomic databases
PaxDbi | O34330 |
PRIDEi | O34330 |
Genome annotation databases
EnsemblBacteriai | CAB13792; CAB13792; BSU19000 |
GeneIDi | 939624 |
KEGGi | bsu:BSU19000 |
PATRICi | fig|224308.179.peg.2078 |
Phylogenomic databases
eggNOGi | COG5444, Bacteria |
OMAi | MKNGNAP |
PhylomeDBi | O34330 |
Enzyme and pathway databases
BioCyci | BSUB:BSU19000-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR026834, LHH IPR006829, LXG_dom |
Pfami | View protein in Pfam PF14411, LHH, 1 hit PF04740, LXG, 1 hit |
PROSITEi | View protein in PROSITE PS51756, LXG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YOBL_BACSU | |
Accessioni | O34330Primary (citable) accession number: O34330 Secondary accession number(s): Q796E2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries