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UniProtKB - O34313 (NTPES_BACSU)
Protein
Trifunctional nucleotide phosphoesterase protein YfkN
Gene
yfkN
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the release of inorganic phosphate from 2',3'-cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'-nucleotidase activity. Does not catalyze the release of inorganic phosphate from 3',5'-cyclic nucleotides. Probably plays a role in the cellular reprocessing of nucleotides present in the medium, under conditions of phosphate shortage.
1 PublicationCatalytic activityi
Cofactori
a divalent metal cationBy similarity
Kineticsi
The 2',3'-cyclic phosphodiesterase activity is higher than that of the 5'-nucleotidase with the four major nucleotides used as substrates.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | Divalent metal cation 1 | 1 | |
Metal bindingi | 54 | Divalent metal cation 1 | 1 | |
Metal bindingi | 97 | Divalent metal cation 1 | 1 | |
Metal bindingi | 97 | Divalent metal cation 2 | 1 | |
Metal bindingi | 141 | Divalent metal cation 2 | 1 | |
Metal bindingi | 249 | Divalent metal cation 2 | 1 | |
Metal bindingi | 282 | Divalent metal cation 2 | 1 | |
Metal bindingi | 284 | Divalent metal cation 1 | 1 | |
Binding sitei | 458 | 3'-ribonucleotideBy similarity | 1 | |
Metal bindingi | 676 | Divalent metal cation 3By similarity | 1 | |
Metal bindingi | 678 | Divalent metal cation 3By similarity | 1 | |
Metal bindingi | 708 | Divalent metal cation 3By similarity | 1 | |
Metal bindingi | 708 | Divalent metal cation 4By similarity | 1 | |
Metal bindingi | 740 | Divalent metal cation 4By similarity | 1 | |
Metal bindingi | 872 | Divalent metal cation 4By similarity | 1 | |
Metal bindingi | 895 | Divalent metal cation 4By similarity | 1 | |
Metal bindingi | 897 | Divalent metal cation 3By similarity | 1 | |
Binding sitei | 1047 | 5'-ribonucleotideBy similarity | 1 |
GO - Molecular functioni
- 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: UniProtKB-EC
- 3'-nucleotidase activity Source: UniProtKB-EC
- 5'-nucleotidase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleotide catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme |
Ligand | Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU07840-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yfkN Ordered Locus Names:BSU07840 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cell wall
- cell wall 1 Publication2 Publications; Peptidoglycan-anchor 1 Publication
Note: Anchored to the cell wall by sortase SrtD.2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- outer membrane-bounded periplasmic space Source: GO_Central
Keywords - Cellular componenti
Cell wall, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | 1 PublicationAdd BLAST | 35 | |
ChainiPRO_0000390884 | 36 – 1427 | Trifunctional nucleotide phosphoesterase protein YfkNAdd BLAST | 1392 | |
PropeptideiPRO_0000390885 | 1428 – 1462 | Removed by sortasePROSITE-ProRule annotationAdd BLAST | 35 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1427 | Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Peptidoglycan-anchorProteomic databases
PaxDbi | O34313 |
PRIDEi | O34313 |
Expressioni
Inductioni
Expression is induced in response to phosphate starvation in a PhoR-dependent manner.1 Publication
Interactioni
Protein-protein interaction databases
IntActi | O34313, 1 interactor |
STRINGi | 224308.BSU07840 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O34313 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O34313 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 623 | 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidaseAdd BLAST | 588 | |
Regioni | 561 – 567 | 3'-ribonucleotide bindingBy similarity | 7 | |
Regioni | 624 – 1427 | 5'-nucleotidaseAdd BLAST | 804 | |
Regioni | 1127 – 1133 | 5'-ribonucleotide bindingBy similarity | 7 | |
Regioni | 1350 – 1422 | DisorderedSequence analysisAdd BLAST | 73 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1424 – 1428 | LPXTG sorting signalPROSITE-ProRule annotation | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1389 – 1413 | Polar residuesSequence analysisAdd BLAST | 25 |
Domaini
The N-terminal region (amino acids 35-623) is able to catalyze the release of phosphate from 2',3'-cyclic nucleotides, but not from 5'-nucleotides.1 Publication
Sequence similaritiesi
Belongs to the 5'-nucleotidase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0737, Bacteria |
InParanoidi | O34313 |
OMAi | DAQKWYA |
Family and domain databases
CDDi | cd07410, MPP_CpdB_N, 1 hit |
Gene3Di | 3.60.21.10, 2 hits 3.90.780.10, 2 hits |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR041827, CpdB_N IPR019931, LPXTG_anchor IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 2 hits |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 2 hits PF00149, Metallophos, 2 hits |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 2 hits SSF56300, SSF56300, 2 hits |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 2 hits PS00786, 5_NUCLEOTIDASE_2, 1 hit PS50847, GRAM_POS_ANCHORING, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O34313-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIQKRRTHV ENILRILLPP IMILSLILPT PPIHAEESAA PQVHLSILAT
60 70 80 90 100
TDIHANMMDY DYYSDKETAD FGLARTAQLI QKHREQNPNT LLVDNGDLIQ
110 120 130 140 150
GNPLGEYAVK YQKDDIISGT KTHPIISVMN ALKYDAGTLG NHEFNYGLDF
160 170 180 190 200
LDGTIKGADF PIVNANVKTT SGENRYTPYV INEKTLIDEN GNEQKVKVGY
210 220 230 240 250
IGFVPPQIMT WDKKNLEGQV QVQDIVESAN ETIPKMKAEG ADVIIALAHT
260 270 280 290 300
GIEKQAQSSG AENAVFDLAT KTKGIDAIIS GHQHGLFPSA EYAGVAQFNV
310 320 330 340 350
EKGTINGIPV VMPSSWGKYL GVIDLKLEKA DGSWKVADSK GSIESIAGNV
360 370 380 390 400
TSRNETVTNT IQQTHQNTLE YVRKPVGKTE ADINSFFAQV KDDPSIQIVT
410 420 430 440 450
DAQKWYAEKE MKDTEYKNLP ILSAGAPFKA GGRNGANYYT NIPAGDLAIK
460 470 480 490 500
NVGDLYLYDN TVQIVKLTGS EVKDWLEMSA GQFNQIDPAK GGDQALLNEN
510 520 530 540 550
FRSYNFDVID GVTYQVDVTK PAKYNENGKV INADSSRIIN LSYEGKPISP
560 570 580 590 600
SQEFLVVTNN YRASGGGGFP HLTSDKIVHG SAVENRQVLM DYIIEQKTVN
610 620 630 640 650
PKADNNWSIA PVSGTNLTFE SSLLAKPFAD KADDVAYVGK SANEGYGVYK
660 670 680 690 700
LQFDDDSNPD PPKDGLWDLT VMHTNDTHAH LDDAARRMTK INEVRSETNH
710 720 730 740 750
NILLDAGDVF SGDLYFTKWN GLADLKMMNM MGYDAMTFGN HEFDKGPTVL
760 770 780 790 800
SDFLSGNSAT VDPANRYHFE APEFPIVSAN VDVSNEPKLK SFVKKPQTFT
810 820 830 840 850
AGEKKEAGIH PYILLDVDGE KVAVFGLTTE DTATTSSPGK SIVFNDAFET
860 870 880 890 900
AQNTVKAIQE EEKVNKIIAL THIGHNRDLE LAKKVKGIDL IIGGHTHTLV
910 920 930 940 950
DKMEVVNNEE PTIVAQAKEY GQFLGRVDVA FDEKGVVQTD KSNLSVLPID
960 970 980 990 1000
EHTEENPEAK QELDQFKNEL EDVKNEKVGY TDVALDGQRE HVRTKETNLG
1010 1020 1030 1040 1050
NFIADGMLAK AKEAAGARIA ITNGGGIRAG IDKGDITLGE VLNVMPFGNT
1060 1070 1080 1090 1100
LYVADLTGKQ IKEALEQGLS NVENGGGAFP QVAGIEYTFT LNNKPGHRVL
1110 1120 1130 1140 1150
EVKIESPNGD KVAINTDDTY RVATNNFVGA GGDGYSVFTE ASHGEDLGYV
1160 1170 1180 1190 1200
DYEIFTEQLK KLGNKVSPKV EGRIKEVFLP TKQKDGSWTL DEDKFAIYAK
1210 1220 1230 1240 1250
NANTPFVYYG IHEGSQEKPI NLKVKKDQVK LLKERESDPS LTMFNYWYSM
1260 1270 1280 1290 1300
KMPMANLKTA DTAIGIKSTG ELDVSLSDVY DFTVKQKGKE IKSFKEPVQL
1310 1320 1330 1340 1350
SLRMFDIEEA HNPAIYHVDR KKKAFTKTGH GSVDDDMVTG YTNHFSEYTI
1360 1370 1380 1390 1400
LNSGSNNKPP AFPSDQPTGG DDGNHGGGSD KPGGKQPTDG NGGNDTPPGT
1410 1420 1430 1440 1450
QPTNGSGGNG SGGSGTDGPA GGLLPDTATS MYSILLAGFL ISALGTAMYL
1460
HQRRKQNRAN QA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83967 Genomic DNA Translation: BAA23404.1 AL009126 Genomic DNA Translation: CAB12613.1 |
PIRi | A69809 |
RefSeqi | NP_388665.1, NC_000964.3 WP_010886443.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12613; CAB12613; BSU_07840 |
GeneIDi | 936131 |
KEGGi | bsu:BSU07840 |
PATRICi | fig|224308.43.peg.822 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83967 Genomic DNA Translation: BAA23404.1 AL009126 Genomic DNA Translation: CAB12613.1 |
PIRi | A69809 |
RefSeqi | NP_388665.1, NC_000964.3 WP_010886443.1, NZ_JNCM01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GVE | X-ray | 1.25 | A/B | 37-374 | [»] | |
SMRi | O34313 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O34313, 1 interactor |
STRINGi | 224308.BSU07840 |
Proteomic databases
PaxDbi | O34313 |
PRIDEi | O34313 |
Protocols and materials databases
DNASUi | 936131 |
Genome annotation databases
EnsemblBacteriai | CAB12613; CAB12613; BSU_07840 |
GeneIDi | 936131 |
KEGGi | bsu:BSU07840 |
PATRICi | fig|224308.43.peg.822 |
Phylogenomic databases
eggNOGi | COG0737, Bacteria |
InParanoidi | O34313 |
OMAi | DAQKWYA |
Enzyme and pathway databases
BioCyci | BSUB:BSU07840-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O34313 |
Family and domain databases
CDDi | cd07410, MPP_CpdB_N, 1 hit |
Gene3Di | 3.60.21.10, 2 hits 3.90.780.10, 2 hits |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR041827, CpdB_N IPR019931, LPXTG_anchor IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 2 hits |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 2 hits PF00149, Metallophos, 2 hits |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 2 hits SSF56300, SSF56300, 2 hits |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 2 hits PS00786, 5_NUCLEOTIDASE_2, 1 hit PS50847, GRAM_POS_ANCHORING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NTPES_BACSU | |
Accessioni | O34313Primary (citable) accession number: O34313 Secondary accession number(s): Q79EX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 19, 2010 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families