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Protein

4-hydroxybenzoyl-CoA reductase subunit beta

Gene

hcrB

Organism
Thauera aromatica
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a complex that catalyzes the reductive dehydroxylation of 4-hydroxybenzoyl-CoA to benzoyl-CoA. Reaction is not reversible. Is a key enzyme in the anaerobic degradation of phenolic compounds.1 Publication

Catalytic activityi

Benzoyl-CoA + oxidized ferredoxin = 4-hydroxybenzoyl-CoA + reduced ferredoxin.1 Publication

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Inactivated by low concentrations of cyanide in vitro.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111FAD1 Publication1
Binding sitei115FAD1 Publication1
Binding sitei118FAD1 Publication1
Metal bindingi122Iron-sulfur (4Fe-4S)1
Metal bindingi138Iron-sulfur (4Fe-4S)1
Metal bindingi146Iron-sulfur (4Fe-4S)1
Metal bindingi155Iron-sulfur (4Fe-4S)1
Binding sitei162FAD1 Publication1
Binding sitei224FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 36FAD1 Publication8

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:BOHBENREDTHAUERA-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxybenzoyl-CoA reductase subunit beta (EC:1.3.7.9)
Short name:
4-HBCR subunit beta
Gene namesi
Name:hcrB
OrganismiThauera aromatica
Taxonomic identifieri59405 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeThauera

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839261 – 3244-hydroxybenzoyl-CoA reductase subunit betaAdd BLAST324

Interactioni

Subunit structurei

Heterohexamer of two alpha, two beta and two gamma subunits.2 Publications

Protein-protein interaction databases

DIPiDIP-48467N
IntActiO33820, 1 interactor

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO33820
SMRiO33820
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO33820

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 217FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST216

Phylogenomic databases

KOiK04109

Family and domain databases

Gene3Di3.30.465.10, 1 hit
InterProiView protein in InterPro
IPR017608 4hydrxbenzoyl-CoA_Rdtase_bsu
IPR005107 CO_DH_flav_C
IPR036683 CO_DH_flav_C_dom_sf
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016169 FAD-bd_PCMH_sub2
IPR002346 Mopterin_DH_FAD-bd
PfamiView protein in Pfam
PF03450 CO_deh_flav_C, 1 hit
PF00941 FAD_binding_5, 1 hit
SMARTiView protein in SMART
SM01092 CO_deh_flav_C, 1 hit
SUPFAMiSSF55447 SSF55447, 1 hit
SSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR03195 4hydrxCoA_B, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

O33820-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNILTDFRTH RPATLADAVN ALAAEATLPL GAGTDLLPNL RRGLGHPAAL
60 70 80 90 100
VDLTGIDGLA TISTLADGSL RIGAGATLEA IAEHDAIRTT WPALAQAAES
110 120 130 140 150
VAGPTHRAAA TLGGNLCQDT RCTFYNQSEW WRSGNGYCLK YKGDKCHVIV
160 170 180 190 200
KSDRCYATYH GDVAPALMVL DARAEIVGPA GKRTVPVAQL FRESGAEHLT
210 220 230 240 250
LEKGELLAAI EVPPTGAWSA AYSKVRIRDA VDFPLAGVAA ALQRDGDRIA
260 270 280 290 300
GLRVAITGSN SAPLMVPVDA LLGGNWDDAA AETLAQLVRK TSNVLRTTIT
310 320
GVKYRRRVLL AISRKVVDQL WEAR
Length:324
Mass (Da):34,374
Last modified:January 1, 1998 - v1
Checksum:iC3C5ECAAD093F9DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22 – 30LAAEATLPL → GTVPVAQLF AA sequence (PubMed:9490068).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001830 Genomic DNA Translation: CAA05039.1

Genome annotation databases

KEGGiag:CAA05039

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001830 Genomic DNA Translation: CAA05039.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RM6X-ray1.60B/E1-324[»]
1SB3X-ray2.20B/E1-324[»]
ProteinModelPortaliO33820
SMRiO33820
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48467N
IntActiO33820, 1 interactor

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA05039

Phylogenomic databases

KOiK04109

Enzyme and pathway databases

BioCyciMetaCyc:BOHBENREDTHAUERA-MONOMER

Miscellaneous databases

EvolutionaryTraceiO33820

Family and domain databases

Gene3Di3.30.465.10, 1 hit
InterProiView protein in InterPro
IPR017608 4hydrxbenzoyl-CoA_Rdtase_bsu
IPR005107 CO_DH_flav_C
IPR036683 CO_DH_flav_C_dom_sf
IPR016166 FAD-bd_PCMH
IPR036318 FAD-bd_PCMH-like_sf
IPR016169 FAD-bd_PCMH_sub2
IPR002346 Mopterin_DH_FAD-bd
PfamiView protein in Pfam
PF03450 CO_deh_flav_C, 1 hit
PF00941 FAD_binding_5, 1 hit
SMARTiView protein in SMART
SM01092 CO_deh_flav_C, 1 hit
SUPFAMiSSF55447 SSF55447, 1 hit
SSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR03195 4hydrxCoA_B, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHCRB_THAAR
AccessioniPrimary (citable) accession number: O33820
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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