The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - O33804 (MURF_STRTO)
Protein
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Gene
murF
Organism
Streptomyces toyocaensis
Status
Functioni
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.
UniRule annotationCatalytic activityi
- ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineUniRule annotationEC:6.3.2.10UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 108 – 114 | ATPSequence analysis | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Source: UniProtKB-EC
- UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)Alternative name(s): D-alanyl-D-alanine-adding enzymeUniRule annotation UDP-MurNAc-pentapeptide synthetase |
Gene namesi | Name:murFUniRule annotation Synonyms:murX |
Organismi | Streptomyces toyocaensis |
Taxonomic identifieri | 55952 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101705 | 1 – 446 | UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST | 446 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
OMAi | TIINDAW |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
i Sequence
Sequence statusi: Complete.
O33804-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIPLGLGEIA EIVGGKATGE SVTVTAPAVL DSRQAEPGGL FVAFAGEHAD
60 70 80 90 100
GHDYAERAGR AGAVAVLGSR PTSLPTVVVE DVRTALQTLA AHVVARLRDG
110 120 130 140 150
LTVVGVTGSQ GKTGTKDLLA AVLSGAGPTV ATTGSLNNEL GVPLTMLRAQ
160 170 180 190 200
AATRFLVLEM GARHEGDIAE LTGLVAPDIA VVLNVGVAHL GEFGSRAAIA
210 220 230 240 250
RAKGELVQGL VPGGTAVLNA DDPRVASMSV LTDGPVLTFG RTDPADVQVL
260 270 280 290 300
DVVLDRLGRP SCTLRTVDTS ARLALPLVGA HQALNASAAA AAALAAGVPL
310 320 330 340 350
DLATTALTTA SLSPWRMELR DLACGATLLN DSYNANPDST RAALDALSAI
360 370 380 390 400
EGGRRIAVLG EMLELGDGSE AEHRAVGAYA AARADVVVAV GEAARPLADG
410 420 430 440
AGERAVVLAG NDAAVDWLRD HLAAGDVVLV KASRGARLDE VAAGLA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82965 Genomic DNA Translation: AAC23581.2 |
RefSeqi | WP_037926351.1, NZ_JFCB01000001.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82965 Genomic DNA Translation: AAC23581.2 |
RefSeqi | WP_037926351.1, NZ_JFCB01000001.1 |
3D structure databases
AlphaFoldDBi | O33804 |
SMRi | O33804 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 55952.BU52_01160 |
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
OMAi | TIINDAW |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MURF_STRTO | |
Accessioni | O33804Primary (citable) accession number: O33804 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 17, 2003 | |
Last modified: | May 25, 2022 | |
This is version 107 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families