thiS

Functionⁱ

Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.1 Publication

Pathwayⁱ: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functionⁱ

nucleotide binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

thiamine biosynthetic process Source: UniProtKB-KW
thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Thiamine biosynthesis
Ligand	Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:THIS-MONOMER MetaCyc:THIS-MONOMER
UniPathwayⁱ	UPA00060

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Sulfur carrier protein ThiS Alternative name(s): Thiamine biosynthesis protein ThiS
Gene namesⁱ	Name:thiS Synonyms:thiG1 Ordered Locus Names:b4407, JW3955
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG14363 thiS

EcoGeneⁱ

EG14363 thiS

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000072518	1 – 66	Sulfur carrier protein ThiSAdd BLAST		66

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	66	1-thioglycine; alternate1 Publication	1
Modified residueⁱ	66	Glycyl adenylate; alternate1 Publication	1
Cross-linkⁱ	66	Glycyl cysteine dithioester (Gly-Cys) (interchain with C-184 in ThiF); alternate

Post-translational modificationⁱ

C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.1 Publication

Keywords - PTMⁱ

Phosphoprotein, Thioester bond

Proteomic databases

EPDⁱ	O32583
PaxDbⁱ	O32583
PRIDEⁱ	O32583

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4261404, 4 interactors
ComplexPortalⁱ	CPX-2134 ThiF-ThiS complex
IntActⁱ	O32583, 1 interactor
STRINGⁱ	316385.ECDH10B_4180

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 5	Combined sources	3
Beta strandⁱ	8 – 10	Combined sources	3
Helixⁱ	18 – 25	Combined sources	8
Beta strandⁱ	32 – 36	Combined sources	5
Beta strandⁱ	39 – 41	Combined sources	3
Helixⁱ	43 – 45	Combined sources	3
Turnⁱ	46 – 48	Combined sources	3
Beta strandⁱ	56 – 61	Combined sources	6

3D structure databases

ProteinModelPortalⁱ	O32583
SMRⁱ	O32583
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

O32583

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the sulfur carrier protein ThiS family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105WHD Bacteria COG2104 LUCA
HOGENOMⁱ	HOG000248060
InParanoidⁱ	O32583
KEGG Orthology (KO) More...KOⁱ	K03154
OMAⁱ	MRILFND
PhylomeDBⁱ	O32583

Family and domain databases

Gene3Dⁱ	3.10.20.30, 1 hit
InterProⁱ	View protein in InterPro IPR012675 Beta-grasp_dom_sf IPR016155 Mopterin_synth/thiamin_S_b IPR010035 Thi_S IPR003749 ThiS/MoaD-like
PANTHERⁱ	PTHR34472 PTHR34472, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02597 ThiS, 1 hit
SUPFAMⁱ	SSF54285 SSF54285, 1 hit
TIGRFAMsⁱ	TIGR01683 thiS, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O32583-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQILFNDQAM QCAAGQTVHE LLEQLDQRQA GAALAINQQI VPREQWAQHI 
        60 
VQDGDQILLF QVIAGG

Length:66

Mass (Da):7,311

Last modified:January 1, 1998 - v1

Checksum:ⁱ9AB3C87835758B40

GO

Mass spectrometryⁱ

Molecular mass is 7310.75 Da from positions 1 - 66. Determined by ESI. 1 Publication

Molecular mass is 7310.74 Da from positions 1 - 66. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88701 Genomic DNA Translation: AAB95620.1 U00006 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAT48237.1 AP009048 Genomic DNA Translation: BAE77328.1
PIRⁱ	S77700
NCBI Reference Sequences More...RefSeqⁱ	WP_001166226.1, NZ_LN832404.1 YP_026279.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaⁱ	AAT48237; AAT48237; b4407 BAE77328; BAE77328; BAE77328
GeneIDⁱ	2847702
KEGGⁱ	ecj:JW3955 eco:b4407
PATRICⁱ	fig\|1411691.4.peg.2720

Protein	Similar proteins		Species	Score	Length
O32583	Immediate sulfur donor in thiazole formation		ECOLX		66
Sulfur carrier protein		Escherichia coli G3/10		66
Sulfur carrier protein ThiS		Escherichia coli KTE108		66
Sulfur carrier protein ThiS		Escherichia coli HVH 50 (4-2593475)		66
Thiamine biosynthesis protein ThiS		SHIDY		66
+24

Protein	Similar proteins		Species	Score	Length
O32583	Immediate sulfur donor in thiazole formation		ECOLX		66
Sulfur carrier protein		Escherichia coli G3/10		66
Sulfur carrier protein ThiS		Escherichia coli KTE108		66
Sulfur carrier protein ThiS		Escherichia coli HVH 50 (4-2593475)		66
Thiamine biosynthesis protein ThiS		SHIDY		66
+254

Protein	Similar proteins		Species	Score	Length
O32583	Immediate sulfur donor in thiazole formation		ECOLX		66
Sulfur carrier protein		Escherichia coli G3/10		66
Sulfur carrier protein ThiS		Escherichia coli KTE108		66
Sulfur carrier protein ThiS		Escherichia coli HVH 50 (4-2593475)		66
Thiamine biosynthesis protein ThiS		SHIDY		66
+762

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88701 Genomic DNA Translation: AAB95620.1 U00006 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAT48237.1 AP009048 Genomic DNA Translation: BAE77328.1
PIRⁱ	S77700
NCBI Reference Sequences More...RefSeqⁱ	WP_001166226.1, NZ_LN832404.1 YP_026279.1, NC_000913.3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1F0Z	NMR	-	A	1-66	[»]
	1ZUD	X-ray	1.98	2/4	1-66	[»]
ProteinModelPortalⁱ	O32583
SMRⁱ	O32583
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261404, 4 interactors
ComplexPortalⁱ	CPX-2134 ThiF-ThiS complex
IntActⁱ	O32583, 1 interactor
STRINGⁱ	316385.ECDH10B_4180

Proteomic databases

EPDⁱ	O32583
PaxDbⁱ	O32583
PRIDEⁱ	O32583

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAT48237; AAT48237; b4407 BAE77328; BAE77328; BAE77328
GeneIDⁱ	2847702
KEGGⁱ	ecj:JW3955 eco:b4407
PATRICⁱ	fig\|1411691.4.peg.2720

Organism-specific databases

EchoBASEⁱ	EB4108
EcoGeneⁱ	EG14363 thiS

Phylogenomic databases

eggNOGⁱ	ENOG4105WHD Bacteria COG2104 LUCA
HOGENOMⁱ	HOG000248060
InParanoidⁱ	O32583
KEGG Orthology (KO) More...KOⁱ	K03154
OMAⁱ	MRILFND
PhylomeDBⁱ	O32583

Enzyme and pathway databases

UniPathwayⁱ	UPA00060
BioCycⁱ	EcoCyc:THIS-MONOMER MetaCyc:THIS-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	O32583
Protein Ontology More...PROⁱ	PR:O32583

Family and domain databases

Gene3Dⁱ	3.10.20.30, 1 hit
InterProⁱ	View protein in InterPro IPR012675 Beta-grasp_dom_sf IPR016155 Mopterin_synth/thiamin_S_b IPR010035 Thi_S IPR003749 ThiS/MoaD-like
PANTHERⁱ	PTHR34472 PTHR34472, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02597 ThiS, 1 hit
SUPFAMⁱ	SSF54285 SSF54285, 1 hit
TIGRFAMsⁱ	TIGR01683 thiS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	THIS_ECOLI
Accessionⁱ	O32583Primary (citable) accession number: O32583 Secondary accession number(s): Q2M8S8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	January 1, 1998
	Last modified:	June 20, 2018
	This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

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Supporting data

UniProtKB - O32583 (THIS_ECOLI)

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Sulfur carrier protein ThiS

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: thiamine diphosphate biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: thiamine diphosphate biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Mass spectrometryⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ