UniProtKB - O32583 (THIS_ECOLI)
Protein
Sulfur carrier protein ThiS
Gene
thiS
Organism
Escherichia coli (strain K12)
Status
Functioni
Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.1 Publication
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- thiamine biosynthetic process Source: UniProtKB-KW
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Biological process | Thiamine biosynthesis |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:THIS-MONOMER MetaCyc:THIS-MONOMER |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfur carrier protein ThiSAlternative name(s): Thiamine biosynthesis protein ThiS |
Gene namesi | Name:thiS Synonyms:thiG1 Ordered Locus Names:b4407, JW3955 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072518 | 1 – 66 | Sulfur carrier protein ThiSAdd BLAST | 66 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 66 | 1-thioglycine; alternate1 Publication | 1 | |
Modified residuei | 66 | Glycyl adenylate; alternate1 Publication | 1 | |
Cross-linki | 66 | Glycyl cysteine dithioester (Gly-Cys) (interchain with C-184 in ThiF); alternate |
Post-translational modificationi
C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.1 Publication
Keywords - PTMi
Phosphoprotein, Thioester bondProteomic databases
PaxDbi | O32583 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261404, 4 interactors |
ComplexPortali | CPX-2134, ThiF-ThiS complex |
IntActi | O32583, 1 interactor |
STRINGi | 511145.b4407 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | O32583 |
SMRi | O32583 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O32583 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sulfur carrier protein ThiS family.Curated
Phylogenomic databases
eggNOGi | COG2104, Bacteria |
HOGENOMi | CLU_174611_2_1_6 |
InParanoidi | O32583 |
PhylomeDBi | O32583 |
Family and domain databases
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR012675, Beta-grasp_dom_sf IPR016155, Mopterin_synth/thiamin_S_b IPR010035, Thi_S IPR003749, ThiS/MoaD-like |
PANTHERi | PTHR34472, PTHR34472, 1 hit |
Pfami | View protein in Pfam PF02597, ThiS, 1 hit |
SUPFAMi | SSF54285, SSF54285, 1 hit |
TIGRFAMsi | TIGR01683, thiS, 1 hit |
i Sequence
Sequence statusi: Complete.
O32583-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQILFNDQAM QCAAGQTVHE LLEQLDQRQA GAALAINQQI VPREQWAQHI
60
VQDGDQILLF QVIAGG
Mass spectrometryi
Molecular mass is 7310.75 Da. Determined by ESI. 1 Publication
Molecular mass is 7310.74 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95620.1 U00006 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAT48237.1 AP009048 Genomic DNA Translation: BAE77328.1 |
PIRi | S77700 |
RefSeqi | WP_001166226.1, NZ_SSZK01000047.1 YP_026279.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48237; AAT48237; b4407 BAE77328; BAE77328; BAE77328 |
GeneIDi | 2847702 57730782 |
KEGGi | ecj:JW3955 eco:b4407 |
PATRICi | fig|1411691.4.peg.2720 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88701 Genomic DNA Translation: AAB95620.1 U00006 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAT48237.1 AP009048 Genomic DNA Translation: BAE77328.1 |
PIRi | S77700 |
RefSeqi | WP_001166226.1, NZ_SSZK01000047.1 YP_026279.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F0Z | NMR | - | A | 1-66 | [»] | |
1ZUD | X-ray | 1.98 | 2/4 | 1-66 | [»] | |
BMRBi | O32583 | |||||
SMRi | O32583 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261404, 4 interactors |
ComplexPortali | CPX-2134, ThiF-ThiS complex |
IntActi | O32583, 1 interactor |
STRINGi | 511145.b4407 |
Proteomic databases
PaxDbi | O32583 |
Genome annotation databases
EnsemblBacteriai | AAT48237; AAT48237; b4407 BAE77328; BAE77328; BAE77328 |
GeneIDi | 2847702 57730782 |
KEGGi | ecj:JW3955 eco:b4407 |
PATRICi | fig|1411691.4.peg.2720 |
Organism-specific databases
EchoBASEi | EB4108 |
Phylogenomic databases
eggNOGi | COG2104, Bacteria |
HOGENOMi | CLU_174611_2_1_6 |
InParanoidi | O32583 |
PhylomeDBi | O32583 |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | EcoCyc:THIS-MONOMER MetaCyc:THIS-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O32583 |
PROi | PR:O32583 |
Family and domain databases
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR012675, Beta-grasp_dom_sf IPR016155, Mopterin_synth/thiamin_S_b IPR010035, Thi_S IPR003749, ThiS/MoaD-like |
PANTHERi | PTHR34472, PTHR34472, 1 hit |
Pfami | View protein in Pfam PF02597, ThiS, 1 hit |
SUPFAMi | SSF54285, SSF54285, 1 hit |
TIGRFAMsi | TIGR01683, thiS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIS_ECOLI | |
Accessioni | O32583Primary (citable) accession number: O32583 Secondary accession number(s): Q2M8S8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families