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Protein

Copper-exporting P-type ATPase

Gene

copA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in copper export.1 Publication

Miscellaneous

The copZA operon is activated by CueR and indirectly repressed by YfmP.

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Copper 11
Metal bindingi19Copper 11
Metal bindingi84Copper 21
Metal bindingi87Copper 21
Active sitei4994-aspartylphosphate intermediateBy similarity1
Metal bindingi698MagnesiumPROSITE-ProRule annotation1
Metal bindingi702MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: InterPro
  • copper-transporting ATPase activity Source: GO_Central
  • identical protein binding Source: IntAct

Keywordsi

Molecular functionHydrolase
Biological processCopper transport, Ion transport, Transport
LigandATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU33500-MONOMER
BRENDAi3.6.3.54 658

Protein family/group databases

TCDBi3.A.3.5.18 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-exporting P-type ATPase (EC:3.6.3.54)
Short name:
Protein CopA
Alternative name(s):
Copper-exporting P-type ATPase A
Cu(+)-exporting ATPase
Gene namesi
Name:copA
Synonyms:yvgX
Ordered Locus Names:BSU33500
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei162 – 181HelicalSequence analysisAdd BLAST20
Transmembranei196 – 218HelicalSequence analysisAdd BLAST23
Transmembranei230 – 249HelicalSequence analysisAdd BLAST20
Transmembranei259 – 278HelicalSequence analysisAdd BLAST20
Transmembranei412 – 434HelicalSequence analysisAdd BLAST23
Transmembranei447 – 469HelicalSequence analysisAdd BLAST23
Transmembranei756 – 775HelicalSequence analysisAdd BLAST20
Transmembranei779 – 796HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463301 – 802Copper-exporting P-type ATPaseAdd BLAST802

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO32220
PRIDEiO32220

Expressioni

Inductioni

By Cu2+.1 Publication

Interactioni

Subunit structurei

Monomer at sub-stoichiometric copper concentrations. Homodimer at higher copper concentrations. Forms a heterodimer (electrostatic interactions) with CopZ during the transfer of Cu+.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-905041,EBI-905041

GO - Molecular functioni

Protein-protein interaction databases

IntActiO32220, 2 interactors
MINTiO32220
STRINGi224308.Bsubs1_010100018186

Structurei

Secondary structure

1802
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO32220
SMRiO32220
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO32220

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 72HMA 1PROSITE-ProRule annotationAdd BLAST67
Domaini74 – 140HMA 2PROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C59 Bacteria
COG2217 LUCA
HOGENOMiHOG000250397
InParanoidiO32220
KOiK17686
OMAiHWMLPAW
PhylomeDBiO32220

Family and domain databases

CDDicd00371 HMA, 2 hits
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006122 HMA_Cu_ion-bd
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 2 hits
SUPFAMiSSF55008 SSF55008, 2 hits
SSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
TIGR00003 TIGR00003, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 2 hits
PS50846 HMA_2, 2 hits

Sequencei

Sequence statusi: Complete.

O32220-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEQKEIAMQ VSGMTCAACA ARIEKGLKRM PGVTDANVNL ATETSNVIYD
60 70 80 90 100
PAETGTAAIQ EKIEKLGYHV VTEKAEFDIE GMTCAACANR IEKRLNKIEG
110 120 130 140 150
VANAPVNFAL ETVTVEYNPK EASVSDLKEA VDKLGYKLKL KGEQDSEAAA
160 170 180 190 200
KKKEERKQTA RLIFSAVLSF PLLWAMVSHF TFTSFIWVPD IFLNPWMQFA
210 220 230 240 250
LATPVQFLIG WPFYVGAYKA LRNKSANMDV LVALGTTAAY AYSLYLTFQS
260 270 280 290 300
IGSHGHTDGL YYETSAILLT LILLGKLFET KAKGRSSDAI KKLMKLQAKT
310 320 330 340 350
ATVVRDGQEQ IIPIDEVLVN DIVYVKPGER IPVDGEVVEG RSAVDESMIT
360 370 380 390 400
GESLPVDKNP GDSVTGSTVN ANGFLKIKAV NVGKDTALSH IIKIVEEAQG
410 420 430 440 450
SKAPIQRLAD QISGIFVPIV LGIAVLTFLI WYLWAAPGDF AEAISKFIAV
460 470 480 490 500
LVIACPCALG LATPTSIMAG SGRAAEFGIL FKGGEHLEKT HRLDTIVLDK
510 520 530 540 550
TGTVTNGKPR LTDAIPFGRF EEKDLLQFAA AAETGSEHPL GEAIIAGVKD
560 570 580 590 600
KGLEIPKLTR FEAKVGAGIL AEAGGKSILV GTRKLMESEQ VEHGALLAQM
610 620 630 640 650
EELEAEGKTV MLVSIDGEAA GLVAVADTIK DTSRKAVARL KELGLDVIMM
660 670 680 690 700
TGDNRRTAEA IAKEAGIANI IAEVLPEQKA AEIARLQKEG RQTAMVGDGI
710 720 730 740 750
NDAPALATAD IGMAIGTGTD IAMETADITL IRGDLNSIAD AIRMSRLTMK
760 770 780 790 800
NIKQNLFWAL GYNSLGIPIA ALGFLAPWIA GAAMAFSSVS VVLNALRLQK

VK
Length:802
Mass (Da):85,912
Last modified:June 16, 2009 - v2
Checksum:i8D6EC2B203E11E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15355.2
PIRiE70041
RefSeqiNP_391230.2, NC_000964.3
WP_003242925.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15355; CAB15355; BSU33500
GeneIDi937985
KEGGibsu:BSU33500
PATRICifig|224308.179.peg.3635

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15355.2
PIRiE70041
RefSeqiNP_391230.2, NC_000964.3
WP_003242925.1, NZ_JNCM01000033.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JWWNMR-A71-156[»]
1KQKNMR-A72-146[»]
1OPZNMR-A2-71[»]
1OQ3NMR-A2-71[»]
1OQ6NMR-A2-71[»]
1P6TNMR-A2-146[»]
2RMLNMR-A2-146[»]
2VOYelectron microscopy18.00A71-146[»]
ProteinModelPortaliO32220
SMRiO32220
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO32220, 2 interactors
MINTiO32220
STRINGi224308.Bsubs1_010100018186

Protein family/group databases

TCDBi3.A.3.5.18 the p-type atpase (p-atpase) superfamily

Proteomic databases

PaxDbiO32220
PRIDEiO32220

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15355; CAB15355; BSU33500
GeneIDi937985
KEGGibsu:BSU33500
PATRICifig|224308.179.peg.3635

Phylogenomic databases

eggNOGiENOG4105C59 Bacteria
COG2217 LUCA
HOGENOMiHOG000250397
InParanoidiO32220
KOiK17686
OMAiHWMLPAW
PhylomeDBiO32220

Enzyme and pathway databases

BioCyciBSUB:BSU33500-MONOMER
BRENDAi3.6.3.54 658

Miscellaneous databases

EvolutionaryTraceiO32220

Family and domain databases

CDDicd00371 HMA, 2 hits
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006122 HMA_Cu_ion-bd
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 2 hits
SUPFAMiSSF55008 SSF55008, 2 hits
SSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
TIGR00003 TIGR00003, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 2 hits
PS50846 HMA_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCOPA_BACSU
AccessioniPrimary (citable) accession number: O32220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 16, 2009
Last modified: November 7, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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