The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - O32179 (PROD1_BACSU)
Protein
Proline dehydrogenase 1
Gene
fadM
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Converts proline to delta-1-pyrroline-5-carboxylate.
By similarityCatalytic activityi
- EC:1.5.5.2
Cofactori
FADBy similarity
: L-proline degradation into L-glutamate Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from L-proline. This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | SubstrateBy similarity | 1 | |
Active sitei | 129 | By similarity | 1 | |
Binding sitei | 130 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 158 | FADBy similarity | 1 | |
Active sitei | 179 | By similarity | 1 | |
Sitei | 270 | Critical for catalytic activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 182 – 184 | FADBy similarity | 3 | |
Nucleotide bindingi | 221 – 222 | FADBy similarity | 2 |
GO - Molecular functioni
- FAD binding Source: UniProtKB
- proline dehydrogenase activity Source: UniProtKB
GO - Biological processi
- proline catabolic process Source: UniProtKB
- proline catabolic process to glutamate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Proline metabolism |
Ligand | FAD, Flavoprotein, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU32850-MONOMER |
UniPathwayi | UPA00261;UER00373 |
Names & Taxonomyi
Protein namesi | Recommended name: Proline dehydrogenase 1 (EC:1.5.5.2)Short name: PRODH 1 Alternative name(s): Proline oxidase 1 |
Gene namesi | Name:fadM Synonyms:yusM Ordered Locus Names:BSU32850 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360508 | 1 – 302 | Proline dehydrogenase 1Add BLAST | 302 |
Proteomic databases
PaxDbi | O32179 |
Expressioni
Inductioni
Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize fadR as to its binding to fadR boxes on target DNA and thus derepress transcription.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 283 – 284 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the proline dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG0506, Bacteria |
InParanoidi | O32179 |
OMAi | YKESAAV |
PhylomeDBi | O32179 |
Family and domain databases
InterProi | View protein in InterPro IPR029041, FAD-linked_oxidoreductase-like IPR008219, PRODH_bac_arc IPR002872, Proline_DH_dom IPR015659, Proline_oxidase |
PANTHERi | PTHR13914, PTHR13914, 2 hits |
Pfami | View protein in Pfam PF01619, Pro_dh, 1 hit |
PIRSFi | PIRSF000196, Pro_dehydrog, 1 hit |
SUPFAMi | SSF51730, SSF51730, 1 hit |
i Sequence
Sequence statusi: Complete.
O32179-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRHVFLFLS QNKTLTKFAK AYGTRLGARR FVAGDTIESA VKTVKRLNRS
60 70 80 90 100
GLCATIDYLG EYAASEKEAN QVAEECKKAI QAIAEHQLNS ELSLKLTSIG
110 120 130 140 150
LDLSEELALT HLRAILSVAK QYDVAVTIDM EDYSHYEQTL SIYRQCKQEF
160 170 180 190 200
EKLGTVIQAY LYRAAEDIRK MRDLKPNLRL VKGAYKESAA VAFPDKRGTD
210 220 230 240 250
LHFQSLIKLQ LLSGNYTAVA THDDDIIAFT KQLVAEHQIP ASQFEFQMLY
260 270 280 290 300
GIRPERQKEL AKEGYRMRVY VPYGTDWFSY FMRRIAERPA NAAFVLKGIL
KK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB15274.1 |
PIRi | F70021 |
RefSeqi | NP_391164.1, NC_000964.3 WP_003228569.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15274; CAB15274; BSU_32850 |
GeneIDi | 937064 |
KEGGi | bsu:BSU32850 |
PATRICi | fig|224308.179.peg.3560 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB15274.1 |
PIRi | F70021 |
RefSeqi | NP_391164.1, NC_000964.3 WP_003228569.1, NZ_JNCM01000033.1 |
3D structure databases
AlphaFoldDBi | O32179 |
SMRi | O32179 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU32850 |
Proteomic databases
PaxDbi | O32179 |
Genome annotation databases
EnsemblBacteriai | CAB15274; CAB15274; BSU_32850 |
GeneIDi | 937064 |
KEGGi | bsu:BSU32850 |
PATRICi | fig|224308.179.peg.3560 |
Phylogenomic databases
eggNOGi | COG0506, Bacteria |
InParanoidi | O32179 |
OMAi | YKESAAV |
PhylomeDBi | O32179 |
Enzyme and pathway databases
UniPathwayi | UPA00261;UER00373 |
BioCyci | BSUB:BSU32850-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR029041, FAD-linked_oxidoreductase-like IPR008219, PRODH_bac_arc IPR002872, Proline_DH_dom IPR015659, Proline_oxidase |
PANTHERi | PTHR13914, PTHR13914, 2 hits |
Pfami | View protein in Pfam PF01619, Pro_dh, 1 hit |
PIRSFi | PIRSF000196, Pro_dehydrog, 1 hit |
SUPFAMi | SSF51730, SSF51730, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PROD1_BACSU | |
Accessioni | O32179Primary (citable) accession number: O32179 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families