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UniProtKB - O32143 (XDHE_BACSU)
Protein
Probable xanthine dehydrogenase subunit E
Gene
pucE
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Oxidizes hypoxanthine and xanthine to uric acid.
1 PublicationCatalytic activityi
Cofactori
[2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
: hypoxanthine degradation Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes urate from hypoxanthine. This subpathway is part of the pathway hypoxanthine degradation, which is itself part of Purine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes urate from hypoxanthine, the pathway hypoxanthine degradation and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 57 | Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 60 | Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 72 | Iron-sulfur (2Fe-2S) 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 110 | Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 113 | Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 145 | Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 147 | Iron-sulfur (2Fe-2S) 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- xanthine dehydrogenase activity Source: UniProtKB-EC
GO - Biological processi
- hypoxanthine catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Purine metabolism |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | BSUB:BSU32470-MONOMER |
UniPathwayi | UPA00604;UER00661 UPA00604;UER00662 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable xanthine dehydrogenase subunit E (EC:1.17.1.4)Short name: XDHase subunit E |
Gene namesi | Name:pucE Synonyms:yurB Ordered Locus Names:BSU32470 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166101 | 1 – 173 | Probable xanthine dehydrogenase subunit EAdd BLAST | 173 |
Proteomic databases
PaxDbi | O32143 |
Expressioni
Inductioni
Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
Interactioni
Subunit structurei
Could be composed of four subunits: PucA, PucC, PucD and PucE.
Protein-protein interaction databases
STRINGi | 224308.BSU32470 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 90 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 77 |
Phylogenomic databases
eggNOGi | COG2080, Bacteria |
InParanoidi | O32143 |
OMAi | FIKHDGY |
PhylomeDBi | O32143 |
Family and domain databases
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR012675, Beta-grasp_dom_sf IPR017611, Xanthine_dehydrogenase_esu |
Pfami | View protein in Pfam PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR03198, pucE, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O32143-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDIKEAGPFP VKKEQFRMTV NGQAWEVAAV PTTHLSDLLR KEFQLTGTKV
60 70 80 90 100
SCGIGRCGAC SILIDGKLAN ACMTMAYQAD GHSITTIEGL QKEELDMCQT
110 120 130 140 150
AFLEEGGFQC GYCTPGMIIA LKALFRETPQ PSDKDIEEGL AGNLCRCTGY
160 170
GGIMRSACRI RRELNGGRRE SGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB15237.1 |
PIRi | A70017 |
RefSeqi | NP_391127.1, NC_000964.3 WP_003243276.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15237; CAB15237; BSU_32470 |
GeneIDi | 936680 |
KEGGi | bsu:BSU32470 |
PATRICi | fig|224308.179.peg.3516 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB15237.1 |
PIRi | A70017 |
RefSeqi | NP_391127.1, NC_000964.3 WP_003243276.1, NZ_JNCM01000033.1 |
3D structure databases
AlphaFoldDBi | O32143 |
SMRi | O32143 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU32470 |
Proteomic databases
PaxDbi | O32143 |
Genome annotation databases
EnsemblBacteriai | CAB15237; CAB15237; BSU_32470 |
GeneIDi | 936680 |
KEGGi | bsu:BSU32470 |
PATRICi | fig|224308.179.peg.3516 |
Phylogenomic databases
eggNOGi | COG2080, Bacteria |
InParanoidi | O32143 |
OMAi | FIKHDGY |
PhylomeDBi | O32143 |
Enzyme and pathway databases
UniPathwayi | UPA00604;UER00661 UPA00604;UER00662 |
BioCyci | BSUB:BSU32470-MONOMER |
Family and domain databases
Gene3Di | 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR002888, 2Fe-2S-bd IPR036884, 2Fe-2S-bd_dom_sf IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR012675, Beta-grasp_dom_sf IPR017611, Xanthine_dehydrogenase_esu |
Pfami | View protein in Pfam PF00111, Fer2, 1 hit PF01799, Fer2_2, 1 hit |
SUPFAMi | SSF47741, SSF47741, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR03198, pucE, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | XDHE_BACSU | |
Accessioni | O32143Primary (citable) accession number: O32143 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways