Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Allantoinase

Gene

allB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring.UniRule annotation1 Publication

Catalytic activityi

(S)-allantoin + H2O = allantoate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: (S)-allantoin degradation

This protein is involved in step 1 of the subpathway that synthesizes allantoate from (S)-allantoin.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Allantoinase (allB)
This subpathway is part of the pathway (S)-allantoin degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes allantoate from (S)-allantoin, the pathway (S)-allantoin degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Zinc 1UniRule annotation1
Metal bindingi62Zinc 1UniRule annotation1
Metal bindingi147Zinc 1; via carbamate groupUniRule annotation1
Metal bindingi147Zinc 2; via carbamate groupUniRule annotation1
Metal bindingi183Zinc 2UniRule annotation1
Metal bindingi239Zinc 2UniRule annotation1
Metal bindingi312Zinc 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPurine metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU32410-MONOMER
UniPathwayi
UPA00395;UER00653

Names & Taxonomyi

Protein namesi
Recommended name:
AllantoinaseUniRule annotation (EC:3.5.2.5UniRule annotation)
Alternative name(s):
Allantoin-utilizing enzymeUniRule annotation
Gene namesi
Name:allBUniRule annotation
Synonyms:pucHUniRule annotation, yunH
Ordered Locus Names:BSU32410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659401 – 446AllantoinaseAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.UniRule annotation

Proteomic databases

PaxDbiO32137
PRIDEiO32137

Expressioni

Inductioni

Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or allantoate are added during limiting-nitrogen conditions.1 Publication

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100017581

Structurei

3D structure databases

ProteinModelPortaliO32137
SMRiO32137
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Allantoinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CD3 Bacteria
COG0044 LUCA
HOGENOMiHOG000219146
InParanoidiO32137
KOiK01466
OMAiHFNEPGR
PhylomeDBiO32137

Family and domain databases

Gene3Di2.30.40.10, 3 hits
HAMAPiMF_01645 Hydantoinase, 1 hit
InterProiView protein in InterPro
IPR017593 Allantoinase
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR03178 allantoinase, 1 hit

Sequencei

Sequence statusi: Complete.

O32137-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAYDMVIKGA KAVTPDGVIE ADIVVQNGVI AEIGSDIEAS GTEIIQADGK
60 70 80 90 100
YVFPGVIDCH VHFNEPGRED WEGFETGSQM MAAGGCTTYF DMPLNCIPST
110 120 130 140 150
VTAEHLLAKA ELGRQKSAVD FALWGGLVPG HIEDIRPMAE AGAIGFKAFL
160 170 180 190 200
SKSGTDEFRS VDERTLLKGM AEIAAAGKIL ALHAESDAIT SYLQMVLANK
210 220 230 240 250
GKVDADAYAA SRPEEAEVEA VYRTIQYAKV TGCPVHFVHI STAKAVRLIR
260 270 280 290 300
EAKQEGLDVS VETCPHYVLF SHDDLRQRGS VAKCAPPLRS RQSKETLIET
310 320 330 340 350
LIAGDIDMVS SDHSPCRPSL KREDNMFLSW GGISGGQFTL LGMLELALEH
360 370 380 390 400
QIPFETIAEW TAAAPAKRFG LQKKGRLEAG CDADFVLVSM EPYTVTRESM
410 420 430 440
FAKHKKSIYE GHTFPCSISA TYSKGRCVYN DGEKVTEIDG ALVVPS
Length:446
Mass (Da):48,331
Last modified:January 1, 1998 - v1
Checksum:i48515B6414A5C234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15231.1
PIRiC70016
RefSeqiNP_391121.1, NC_000964.3
WP_003243112.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15231; CAB15231; BSU32410
GeneIDi936662
KEGGibsu:BSU32410
PATRICifig|224308.179.peg.3508

Similar proteinsi

Entry informationi

Entry nameiALLB_BACSU
AccessioniPrimary (citable) accession number: O32137
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again