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Entry version 110 (29 Sep 2021)
Sequence version 1 (01 Jan 1998)
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Protein

SpoIVD-associated factor A

Gene

safA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Probably involved in the assembly of some coat protein components implicated in both lysozyme resistance and germination. Could be required for the assembly of CotG. Associates with SpoIVD during the early stage of coat assembly.

Miscellaneous

Several coat proteins are extracted in reduced amounts from SafA mutant spores, one of which is CotG. SafA mutant spores have abnormal coat layers and have lost their resistance to lysozyme. Their response to L-alanine suggest that they have a defect at a late stage of spore germination. Their response to AGFK also suggests that they have an additional defect at a early stage of spore germination. The mutation of SafA has no effect on vegetative growth and spore resistance to heat and chloroform.
In wild-type cells, during the early stages of sporulation, a product of 43 kDa (the predicted size of SafA) was detected. However, in later stages, a 30-kDa species was the major form of SafA detected. The N-terminus sequence of the 30-kDa product starts at Met-164. It is not known if the smaller form results from proteolysis or from translation initiated at Met-164.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processSporulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU27840-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
SpoIVD-associated factor A
Alternative name(s):
Morphogenetic protein SafA
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:safA
Synonyms:yrbA
Ordered Locus Names:BSU27840
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002460741 – 387SpoIVD-associated factor AAdd BLAST387

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O32062

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transcribed by SigE at time T2 of sporulation.

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Protein interaction database and analysis system

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IntActi
O32062, 1 interactor

STRING: functional protein association networks

More...
STRINGi
224308.BSU27840

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O32062

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 47LysMPROSITE-ProRule annotationAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni49 – 106DisorderedSequence analysisAdd BLAST58
Regioni355 – 387DisorderedSequence analysisAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi72 – 95Basic and acidic residuesSequence analysisAdd BLAST24
Compositional biasi355 – 381Polar residuesSequence analysisAdd BLAST27

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1388, Bacteria

Identification of Orthologs from Complete Genome Data

More...
OMAi
EFKPEFK

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00118, LysM, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.10.350.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR018392, LysM_dom
IPR036779, LysM_dom_sf
IPR014248, Spore_coat_assembly_SafA

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01476, LysM, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00257, LysM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF54106, SSF54106, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02899, spore_safA, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51782, LYSM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O32062-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIHIVQKGD SLWKIAEKYG VDVEEVKKLN TQLSNPDLIM PGMKIKVPSE
60 70 80 90 100
GVPVRKEPKA GKSPAAGSVK QEHPYAKEKP KSVVDVEDTK PKEKKSMPYV
110 120 130 140 150
PPMPNLQENV YPEADVNDYY DMKQLFQPWS PPKPEEPKKH HDGNMDHMYH
160 170 180 190 200
MQDQFPQQEA MSNMENANYP NMPNMPKAPE VGGIEEENVH HTVPNMPMPA
210 220 230 240 250
VQPYYHYPAH FVPCPVPVSP ILPGSGLCYP YYPAQAYPMH PMHGYQPGFV
260 270 280 290 300
SPQYDPGYEN QHHENSHHGH YGSYGAPQYA SPAYGSPYGH MPYGPYYGTP
310 320 330 340 350
QVMGAYQPAA AHGYMPYKDH DDCGCDGDHQ PYFSAPGHSG MGAYGSPNMP
360 370 380
YGTANPNPNP YSAGVSMPMT NQPSVNQMFG RPEEENE
Length:387
Mass (Da):43,229
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCE619293E809E5D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti184I → V in BAA24943 (PubMed:9770294).Curated1
Sequence conflicti237Y → F in BAA24943 (PubMed:9770294).Curated1
Sequence conflicti266S → T in BAA24943 (PubMed:9770294).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Y15896 Genomic DNA Translation: CAB75322.1
D50551 Genomic DNA Translation: BAA24943.1
AL009126 Genomic DNA Translation: CAB14744.1

Protein sequence database of the Protein Information Resource

More...
PIRi
H69971

NCBI Reference Sequences

More...
RefSeqi
NP_390662.1, NC_000964.3
WP_003229695.1, NZ_JNCM01000036.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB14744; CAB14744; BSU_27840

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
937514

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU27840

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.3025

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15896 Genomic DNA Translation: CAB75322.1
D50551 Genomic DNA Translation: BAA24943.1
AL009126 Genomic DNA Translation: CAB14744.1
PIRiH69971
RefSeqiNP_390662.1, NC_000964.3
WP_003229695.1, NZ_JNCM01000036.1

3D structure databases

SMRiO32062
ModBaseiSearch...

Protein-protein interaction databases

IntActiO32062, 1 interactor
STRINGi224308.BSU27840

Proteomic databases

PaxDbiO32062

Genome annotation databases

EnsemblBacteriaiCAB14744; CAB14744; BSU_27840
GeneIDi937514
KEGGibsu:BSU27840
PATRICifig|224308.179.peg.3025

Phylogenomic databases

eggNOGiCOG1388, Bacteria
OMAiEFKPEFK

Enzyme and pathway databases

BioCyciBSUB:BSU27840-MONOMER

Family and domain databases

CDDicd00118, LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR018392, LysM_dom
IPR036779, LysM_dom_sf
IPR014248, Spore_coat_assembly_SafA
PfamiView protein in Pfam
PF01476, LysM, 1 hit
SMARTiView protein in SMART
SM00257, LysM, 1 hit
SUPFAMiSSF54106, SSF54106, 1 hit
TIGRFAMsiTIGR02899, spore_safA, 1 hit
PROSITEiView protein in PROSITE
PS51782, LYSM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSAFA_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O32062
Secondary accession number(s): O52861, Q799D6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 1, 1998
Last modified: September 29, 2021
This is version 110 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome
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