UniProtKB - O32047 (SECDF_BACSU)
Protein
Protein translocase subunit SecDF
Gene
secDF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for efficient translocation of secretory pre-proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane.1 Publication
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity).By similarity
GO - Molecular functioni
GO - Biological processi
- intracellular protein transmembrane transport Source: UniProtKB-UniRule
- protein targeting Source: UniProtKB-UniRule
- protein transport Source: GO_Central
- protein transport by the Sec complex Source: UniProtKB-UniRule
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
BioCyci | BSUB:BSU27650-MONOMER |
Protein family/group databases
TCDBi | 2.A.6.4.2, the resistance-nodulation-cell division (rnd) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Protein translocase subunit SecDF |
Gene namesi | Name:secDF Ordered Locus Names:BSU27650 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication Note: Found uniformly distributed in both the mother cell and forespore following sporulation.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | Cytoplasmic1 Publication | 5 | |
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 254 | Extracellular1 PublicationAdd BLAST | 228 | |
Transmembranei | 255 – 275 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 276 – 278 | Cytoplasmic1 Publication | 3 | |
Transmembranei | 279 – 299 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 300 – 301 | Extracellular1 Publication | 2 | |
Transmembranei | 302 – 322 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 323 – 353 | Cytoplasmic1 PublicationAdd BLAST | 31 | |
Transmembranei | 354 – 374 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 375 – 380 | Extracellular1 Publication | 6 | |
Transmembranei | 381 – 401 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 402 – 453 | Cytoplasmic1 PublicationAdd BLAST | 52 | |
Transmembranei | 454 – 474 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 475 – 569 | Extracellular1 PublicationAdd BLAST | 95 | |
Transmembranei | 570 – 590 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 591 – 593 | Cytoplasmic1 Publication | 3 | |
Transmembranei | 594 – 614 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 615 – 621 | Extracellular1 Publication | 7 | |
Transmembranei | 622 – 642 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 643 – 677 | Cytoplasmic1 PublicationAdd BLAST | 35 | |
Transmembranei | 678 – 698 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 699 | Extracellular1 Publication | 1 | |
Transmembranei | 700 – 720 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 721 – 737 | Cytoplasmic1 PublicationAdd BLAST | 17 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene show a cold-sensitive phenotype and a filamentous morphology.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000384402 | 1 – 737 | Protein translocase subunit SecDFAdd BLAST | 737 |
Proteomic databases
jPOSTi | O32047 |
PaxDbi | O32047 |
PRIDEi | O32047 |
Expressioni
Inductioni
Shows maximal expression at the beginning of post-exponential growth phase and increased expression by glucose in the post-exponential growth phase when cells are cultured on rich medium. Expressed constitutively during growth in minimal medium.1 Publication
Interactioni
Subunit structurei
Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 224308.BSU27650 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 416 | SecDAdd BLAST | 416 | |
Regioni | 446 – 737 | SecFAdd BLAST | 292 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0341, Bacteria COG0342, Bacteria |
InParanoidi | O32047 |
OMAi | RTVNTGM |
PhylomeDBi | O32047 |
Family and domain databases
HAMAPi | MF_01463_B, SecD_B, 1 hit MF_01464_B, SecF_B, 1 hit |
InterProi | View protein in InterPro IPR005791, SecD IPR022813, SecD/SecF_arch_bac IPR022645, SecD/SecF_bac IPR022646, SecD/SecF_CS IPR005665, SecF_bac |
PANTHERi | PTHR30081, PTHR30081, 2 hits |
Pfami | View protein in Pfam PF07549, Sec_GG, 1 hit PF02355, SecD_SecF, 2 hits |
PRINTSi | PR01755, SECFTRNLCASE |
TIGRFAMsi | TIGR00916, 2A0604s01, 2 hits TIGR00966, 3a0501s07, 1 hit TIGR01129, secD, 1 hit |
i Sequence
Sequence statusi: Complete.
O32047-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKGRLIAFF LFVLLIGTGL GYFTKPAANN ITLGLDLQGG FEVLYDVQPV
60 70 80 90 100
KKGDKITKDV LVSTVEALNR RANVLGVSEP NIQIEGNNRI RVQLAGVTNQ
110 120 130 140 150
NRAREILATE AQLSFRDAND KELLNGADLV ENGAKQTYDS TTNEPIVTIK
160 170 180 190 200
LKDADKFGEV TKKVMKMAPN NQLVIWLDYD KGDSFKKEVQ KEHPKFVSAP
210 220 230 240 250
NVSQELNTTD VKIEGHFTAQ EAKDLASILN AGALPVKLTE KYSTSVGAQF
260 270 280 290 300
GQQALHDTVF AGIVGIAIIF LFMLFYYRLP GLIAVITLSV YIYITLQIFD
310 320 330 340 350
WMNAVLTLPG IAALILGVGM AVDANIITYE RIKEELKLGK SVRSAFRSGN
360 370 380 390 400
RRSFATIFDA NITTIIAAVV LFIFGTSSVK GFATMLILSI LTSFITAVFL
410 420 430 440 450
SRFLLALLVE SRWLDRKKGW FGVNKKHIMD IQDTDENTEP HTPFQKWDFT
460 470 480 490 500
SKRKYFFIFS SAVTVAGIII LLVFRLNLGI DFASGARIEV QSDHKLTTEQ
510 520 530 540 550
VEKDFESLGM DPDTVVLSGE KSNIGVARFV GVPDKETIAK VKTYFKDKYG
560 570 580 590 600
SDPNVSTVSP TVGKELARNA LYAVAIASIG IIIYVSIRFE YKMAIAAIAS
610 620 630 640 650
LLYDAFFIVT FFSITRLEVD VTFIAAILTI IGYSINDTIV TFDRVREHMK
660 670 680 690 700
KRKPKTFADL NHIVNLSLQQ TFTRSINTVL TVVIVVVTLL IFGASSITNF
710 720 730
SIALLVGLLT GVYSSLYIAA QIWLAWKGRE LKKDSAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024506 Genomic DNA Translation: AAC31122.1 AL009126 Genomic DNA Translation: CAB14724.2 |
PIRi | H69704 |
RefSeqi | NP_390643.1, NC_000964.3 WP_003245970.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14724; CAB14724; BSU27650 |
GeneIDi | 937938 |
KEGGi | bsu:BSU27650 |
PATRICi | fig|224308.179.peg.3004 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024506 Genomic DNA Translation: AAC31122.1 AL009126 Genomic DNA Translation: CAB14724.2 |
PIRi | H69704 |
RefSeqi | NP_390643.1, NC_000964.3 WP_003245970.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | O32047 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU27650 |
Protein family/group databases
TCDBi | 2.A.6.4.2, the resistance-nodulation-cell division (rnd) superfamily |
Proteomic databases
jPOSTi | O32047 |
PaxDbi | O32047 |
PRIDEi | O32047 |
Genome annotation databases
EnsemblBacteriai | CAB14724; CAB14724; BSU27650 |
GeneIDi | 937938 |
KEGGi | bsu:BSU27650 |
PATRICi | fig|224308.179.peg.3004 |
Phylogenomic databases
eggNOGi | COG0341, Bacteria COG0342, Bacteria |
InParanoidi | O32047 |
OMAi | RTVNTGM |
PhylomeDBi | O32047 |
Enzyme and pathway databases
BioCyci | BSUB:BSU27650-MONOMER |
Family and domain databases
HAMAPi | MF_01463_B, SecD_B, 1 hit MF_01464_B, SecF_B, 1 hit |
InterProi | View protein in InterPro IPR005791, SecD IPR022813, SecD/SecF_arch_bac IPR022645, SecD/SecF_bac IPR022646, SecD/SecF_CS IPR005665, SecF_bac |
PANTHERi | PTHR30081, PTHR30081, 2 hits |
Pfami | View protein in Pfam PF07549, Sec_GG, 1 hit PF02355, SecD_SecF, 2 hits |
PRINTSi | PR01755, SECFTRNLCASE |
TIGRFAMsi | TIGR00916, 2A0604s01, 2 hits TIGR00966, 3a0501s07, 1 hit TIGR01129, secD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SECDF_BACSU | |
Accessioni | O32047Primary (citable) accession number: O32047 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 22, 2009 |
Last sequence update: | August 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families