UniProtKB - O32038 (SYDND_BACSU)
Protein
Aspartate--tRNA(Asp/Asn) ligase
Gene
aspS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation
Catalytic activityi
- EC:6.1.1.23UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 84 | Important for tRNA non-discriminationUniRule annotation | 1 | |
Binding sitei | 177 | AspartateUniRule annotation | 1 | |
Binding sitei | 223 | AspartateUniRule annotation | 1 | |
Binding sitei | 232 | ATPUniRule annotation | 1 | |
Binding sitei | 451 | AspartateUniRule annotation | 1 | |
Binding sitei | 485 | ATPUniRule annotation | 1 | |
Binding sitei | 492 | AspartateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 223 – 225 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 537 – 540 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
- aspartate-tRNA ligase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- nucleic acid binding Source: InterPro
GO - Biological processi
- aspartyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU27550-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)Alternative name(s): Aspartyl-tRNA synthetaseUniRule annotation Short name: AspRSUniRule annotation Non-discriminating aspartyl-tRNA synthetaseUniRule annotation Short name: ND-AspRSUniRule annotation |
Gene namesi | Name:aspSUniRule annotation Ordered Locus Names:BSU27550 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110829 | 1 – 592 | Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST | 592 |
Proteomic databases
jPOSTi | O32038 |
PaxDbi | O32038 |
PRIDEi | O32038 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
IntActi | O32038, 1 interactor |
MINTi | O32038 |
STRINGi | 224308.BSU27550 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 201 – 204 | AspartateUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
InParanoidi | O32038 |
OMAi | YQLDVEM |
PhylomeDBi | O32038 |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
O32038-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFGRTYYCGD ITEKAIGESV TLKGWVQKRR DLGGLIFIDL RDRTGIVQVV
60 70 80 90 100
FNPDVSKEAL AIAEGIRNEY VLDIQGKVVA REEGTVNPNL KTGAIEIHAD
110 120 130 140 150
GVNVLNAAKT PPFAISDQAE EVSEDVRLKH RYLDLRRPAM FQTMQLRHNV
160 170 180 190 200
TKAVRSFLDE NGFLDIETPI LTGSTPEGAR DYLVPSRVHE GEFYALPQSP
210 220 230 240 250
QLFKQLLMVS GIERYYQIAR CFRDEDLRAD RQPEFTQIDI EMSFMSQEDI
260 270 280 290 300
MSLAEEMMAK VMRETKGEEL QLPLPRMTYD EAMNKYGSDK PDTRFDMLLT
310 320 330 340 350
DVSDIVKDTE FKVFSSAVAN GGVVKAINVK GGAGDYSRKD IDALGAFAAN
360 370 380 390 400
YGAKGLAWVK VEADGVKGPI AKFFDEEKQS KLIEALDAAE GDLLLFGADQ
410 420 430 440 450
FEVVAASLGA LRLKLGKERG LIDEKLFNFL WVIDWPLLEH DPEEGRFYAA
460 470 480 490 500
HHPFTMPVRE DLELIETAPE DMKAQAYDLV LNGYELGGGS IRIFEKDIQE
510 520 530 540 550
KMFALLGFSP EEAAEQFGFL LEAFEYGAPP HGGIALGLDR LVMLLAGRTN
560 570 580 590
LRDTIAFPKT ASASCLMTEA PGEVSDAQLD ELHLSIKKKV KN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB14714.1 |
PIRi | D69591 |
RefSeqi | NP_390633.1, NC_000964.3 WP_003229758.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14714; CAB14714; BSU27550 |
GeneIDi | 937549 |
KEGGi | bsu:BSU27550 |
PATRICi | fig|224308.179.peg.2994 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB14714.1 |
PIRi | D69591 |
RefSeqi | NP_390633.1, NC_000964.3 WP_003229758.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | O32038 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O32038, 1 interactor |
MINTi | O32038 |
STRINGi | 224308.BSU27550 |
Proteomic databases
jPOSTi | O32038 |
PaxDbi | O32038 |
PRIDEi | O32038 |
Genome annotation databases
EnsemblBacteriai | CAB14714; CAB14714; BSU27550 |
GeneIDi | 937549 |
KEGGi | bsu:BSU27550 |
PATRICi | fig|224308.179.peg.2994 |
Phylogenomic databases
eggNOGi | COG0173, Bacteria |
InParanoidi | O32038 |
OMAi | YQLDVEM |
PhylomeDBi | O32038 |
Enzyme and pathway databases
BioCyci | BSUB:BSU27550-MONOMER |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYDND_BACSU | |
Accessioni | O32038Primary (citable) accession number: O32038 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families