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Entry version 125 (12 Aug 2020)
Sequence version 1 (01 Jan 1998)
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Protein

Plipastatin synthase subunit E

Gene

ppsE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

This protein is a multifunctional enzyme, able to activate and polymerize the amino acid Ile as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The activation sites for this amino acid consist of individual domains.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 1 phosphopantetheine covalently.Curated

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processAntibiotic biosynthesis

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU18300-MONOMER

Protein family/group databases

ESTHER database of the Alpha/Beta-hydrolase fold superfamily of proteins

More...
ESTHERi
bacsu-PPSE, Thioesterase

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Plipastatin synthase subunit E (EC:2.3.1.-)
Alternative name(s):
Peptide synthase 5
Including the following 1 domains:
ATP-dependent isoleucine adenylase
Short name:
IsoA
Alternative name(s):
Isoleucine activase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ppsE
Synonyms:pps5
Ordered Locus Names:BSU18300
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003608451 – 1279Plipastatin synthase subunit EAdd BLAST1279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1010O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O31827

PRoteomics IDEntifications database

More...
PRIDEi
O31827

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU18300

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O31827

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini975 – 1050CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni14 – 1047Domain (isoleucine-activating)Add BLAST1034
Regioni14 – 317CondensationAdd BLAST304
Regioni503 – 898AdenylationAdd BLAST396
Regioni1068 – 1273ThioesteraseAdd BLAST206

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1020, Bacteria
COG3319, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O31827

KEGG Orthology (KO)

More...
KOi
K15668

Identification of Orthologs from Complete Genome Data

More...
OMAi
AVYNCYG

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O31827

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.1200.10, 1 hit
3.30.559.10, 1 hit
3.40.50.12780, 1 hit
3.40.50.1820, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR010071, AA_adenyl_domain
IPR029058, AB_hydrolase
IPR036736, ACP-like_sf
IPR025110, AMP-bd_C
IPR020459, AMP-binding
IPR020845, AMP-binding_CS
IPR000873, AMP-dep_Synth/Lig
IPR042099, AMP-dep_Synthh-like_sf
IPR023213, CAT-like_dom_sf
IPR001242, Condensatn
IPR020806, PKS_PP-bd
IPR020802, PKS_thioesterase
IPR009081, PP-bd_ACP
IPR006162, Ppantetheine_attach_site
IPR001031, Thioesterase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00501, AMP-binding, 1 hit
PF13193, AMP-binding_C, 1 hit
PF00668, Condensation, 1 hit
PF00550, PP-binding, 1 hit
PF00975, Thioesterase, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00154, AMPBINDING

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00823, PKS_PP, 1 hit
SM00824, PKS_TE, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47336, SSF47336, 1 hit
SSF53474, SSF53474, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01733, AA-adenyl-dom, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00455, AMP_BINDING, 1 hit
PS50075, CARRIER, 1 hit
PS00012, PHOSPHOPANTETHEINE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O31827-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKGADTMNT IKKIKNIYPL SHMQEGMLFH SFLRKEEGAY VEQSLFTIKG
60 70 80 90 100
SLSYDWFQRS IQAIIDRHDI FRTVFLPHVP HLSGPRQVVM TEREFHLNSE
110 120 130 140 150
DISHLPTNDQ NEYIERFKEK DKQKGFDLQK DMLMRISLFK TAKDEHVCIW
160 170 180 190 200
SHHHILMDGW CLGIVMQEFM QIYQSIHAGK PLSLDPVRPY STYISWLTNR
210 220 230 240 250
DKEKAAAYWD TYLKNYSAPS PLPRVSDKET KESYHREDLI FSLNKPLTDK
260 270 280 290 300
LKETAKQHGV TLATLIQAVW GVMLQQYNRT DDVVFGAVVS GRPSEIPGVE
310 320 330 340 350
QMIGLFINTI PIRIKTHQDE TFHELLIRCQ KEMLEAEPFT CQPLFDIQAN
360 370 380 390 400
TALKQELIDH IIVFENYPLQ QKIADSADQT DSPLQIDQVQ VSEQSGYNFN
410 420 430 440 450
LVVAPGEELV IKFSYNAFVY DAAWISCIKR QFTQALSTAA QHPHMPIADF
460 470 480 490 500
SFLDATEKEQ IVTQFNNTKT EYPKNHTIID LFREQAEKTP DHTALVYGNM
510 520 530 540 550
SISYKELDKR SNALARELIQ KGFRKNETAG ILAAHSPEFM ISVLAVLKAG
560 570 580 590 600
GAYLPLDAEL PPERVSFMLE ETQAKMLIVQ KGLEQNAAFS GTCIISDAQG
610 620 630 640 650
LMEENDIPIN ISSSPDDLAY IMYTSGSTGR PKGVMITNRN VVSLVRNSNY
660 670 680 690 700
TSASGDDRFI MTGSISFDAV TFEMFGALLN GASLHIIDKS TMLTPDRFGA
710 720 730 740 750
YLLENDITVL FLTTALFNQL AQVRADMFRG LHTLYVGGEA LSPALMNAVR
760 770 780 790 800
HACPDLALHN IYGPTENTTF STFFEMKRDY AGPIPIGKPI SNSTAYILDT
810 820 830 840 850
KGRLLPIGVP GELCVGGDGV AKGYLNRVDL TNAVFSPHPF LPGERIYRTG
860 870 880 890 900
DLARWLPDGN LEYISRIDRQ MKIRGKRIEP AEIEARLLEM EGVQEAAVTL
910 920 930 940 950
REKDGEAQLY THYVGDHKKT DTDFRADLAR VLPDYMIPQH WVRVERMPLT
960 970 980 990 1000
GNGKIDRSAL PIPENKPAKR QNIILPRNLV EEELANIWKQ VLGVNTISID
1010 1020 1030 1040 1050
DDFFAIGGHS LRALQVIHTL KHQQNIDIPI DFLFEHPTIA QLAEKLYSKQ
1060 1070 1080 1090 1100
LTAANEQHVI KLNQHGAQNL FCFPPISGFG IYFKDLALLL NEKAAVYGFH
1110 1120 1130 1140 1150
FIEQDTRIEQ YVNCMTDIQP EGPYVLLGYS AGGNLAFEVA QAMERKGLEV
1160 1170 1180 1190 1200
SDFIIVDAYL KEQPLPIDTG NDESAAYLPE AVREKVMKKK RNYQEYWAQL
1210 1220 1230 1240 1250
LNEGHIKASI HFIEAGIHPE TSGHTGLTKW EGACGNYSEY TGFGAHKDML
1260 1270
EGTYAEKNAD IILDILEKIT SNQVILHKR
Length:1,279
Mass (Da):144,619
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i22EA638DEF0CEBE0
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA74213 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Y13917 Genomic DNA Translation: CAA74213.1 Different initiation.
AL009126 Genomic DNA Translation: CAB13713.1

Protein sequence database of the Protein Information Resource

More...
PIRi
E69681

NCBI Reference Sequences

More...
RefSeqi
NP_389712.1, NC_000964.3
WP_010886522.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB13713; CAB13713; BSU18300

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
939956

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU18300

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.43.peg.1940

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13917 Genomic DNA Translation: CAA74213.1 Different initiation.
AL009126 Genomic DNA Translation: CAB13713.1
PIRiE69681
RefSeqiNP_389712.1, NC_000964.3
WP_010886522.1, NZ_JNCM01000035.1

3D structure databases

SMRiO31827
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU18300

Protein family/group databases

ESTHERibacsu-PPSE, Thioesterase

Proteomic databases

PaxDbiO31827
PRIDEiO31827

Genome annotation databases

EnsemblBacteriaiCAB13713; CAB13713; BSU18300
GeneIDi939956
KEGGibsu:BSU18300
PATRICifig|224308.43.peg.1940

Phylogenomic databases

eggNOGiCOG1020, Bacteria
COG3319, Bacteria
InParanoidiO31827
KOiK15668
OMAiAVYNCYG
PhylomeDBiO31827

Enzyme and pathway databases

BioCyciBSUB:BSU18300-MONOMER

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.30.559.10, 1 hit
3.40.50.12780, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR010071, AA_adenyl_domain
IPR029058, AB_hydrolase
IPR036736, ACP-like_sf
IPR025110, AMP-bd_C
IPR020459, AMP-binding
IPR020845, AMP-binding_CS
IPR000873, AMP-dep_Synth/Lig
IPR042099, AMP-dep_Synthh-like_sf
IPR023213, CAT-like_dom_sf
IPR001242, Condensatn
IPR020806, PKS_PP-bd
IPR020802, PKS_thioesterase
IPR009081, PP-bd_ACP
IPR006162, Ppantetheine_attach_site
IPR001031, Thioesterase
PfamiView protein in Pfam
PF00501, AMP-binding, 1 hit
PF13193, AMP-binding_C, 1 hit
PF00668, Condensation, 1 hit
PF00550, PP-binding, 1 hit
PF00975, Thioesterase, 1 hit
PRINTSiPR00154, AMPBINDING
SMARTiView protein in SMART
SM00823, PKS_PP, 1 hit
SM00824, PKS_TE, 1 hit
SUPFAMiSSF47336, SSF47336, 1 hit
SSF53474, SSF53474, 1 hit
TIGRFAMsiTIGR01733, AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455, AMP_BINDING, 1 hit
PS50075, CARRIER, 1 hit
PS00012, PHOSPHOPANTETHEINE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPPSE_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O31827
Secondary accession number(s): Q799M0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 1, 1998
Last modified: August 12, 2020
This is version 125 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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