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UniProtKB - O31743 (RBGA_BACSU)
Protein
Ribosome biogenesis GTPase A
Gene
rbgA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.
4 PublicationsMiscellaneous
Estimated to be present at 1000 copies per cell.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 174 | GTP; via amide nitrogen | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 58 – 61 | GTP | 4 | |
Nucleotide bindingi | 86 – 87 | GTP | 2 | |
Nucleotide bindingi | 130 – 135 | GTP | 6 |
GO - Molecular functioni
- GTPase activity Source: CACAO
- GTP binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- ribosome biogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU16050-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosome biogenesis GTPase A |
Gene namesi | Name:rbgA Synonyms:ylqF Ordered Locus Names:BSU16050 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Essential for growth, it cannot be disrupted. In depletion experiments cells become over 3-fold longer, are abnormally curved and nucleoids condense.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360833 | 1 – 282 | Ribosome biogenesis GTPase AAdd BLAST | 282 |
Proteomic databases
PaxDbi | O31743 |
PRIDEi | O31743 |
Interactioni
Subunit structurei
Interacts with ctc.
Interacts with the immature 50S ribosome subunit. 2 molecules of RbgA bind to one 50S subunit.
2 PublicationsProtein-protein interaction databases
STRINGi | 224308.BSU16050 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O31743 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O31743 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 178 | CP-type GPROSITE-ProRule annotationAdd BLAST | 165 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1161, Bacteria |
InParanoidi | O31743 |
OMAi | GVLWPKF |
PhylomeDBi | O31743 |
Family and domain databases
Gene3Di | 1.10.1580.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR006073, GTP-bd IPR023179, GTP-bd_ortho_bundle_sf IPR019991, GTP-bd_ribosome_bgen IPR016478, GTPase_MTG1 IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit |
PIRSFi | PIRSF006230, MG442, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR03596, GTPase_YlqF, 1 hit |
PROSITEi | View protein in PROSITE PS51721, G_CP, 1 hit |
i Sequence
Sequence statusi: Complete.
O31743-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTIQWFPGHM AKARREVTEK LKLIDIVYEL VDARIPMSSR NPMIEDILKN
60 70 80 90 100
KPRIMLLNKA DKADAAVTQQ WKEHFENQGI RSLSINSVNG QGLNQIVPAS
110 120 130 140 150
KEILQEKFDR MRAKGVKPRA IRALIIGIPN VGKSTLINRL AKKNIAKTGD
160 170 180 190 200
RPGITTSQQW VKVGKELELL DTPGILWPKF EDELVGLRLA VTGAIKDSII
210 220 230 240 250
NLQDVAVFGL RFLEEHYPER LKERYGLDEI PEDIAELFDA IGEKRGCLMS
260 270 280
GGLINYDKTT EVIIRDIRTE KFGRLSFEQP TM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13478.1 |
PIRi | F69880 |
RefSeqi | NP_389487.1, NC_000964.3 WP_009967235.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13478; CAB13478; BSU_16050 |
GeneIDi | 940134 |
KEGGi | bsu:BSU16050 |
PATRICi | fig|224308.179.peg.1745 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13478.1 |
PIRi | F69880 |
RefSeqi | NP_389487.1, NC_000964.3 WP_009967235.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PUJ | X-ray | 2.00 | A | 1-282 | [»] | |
6PPK | electron microscopy | 4.40 | W | 1-282 | [»] | |
SMRi | O31743 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU16050 |
Proteomic databases
PaxDbi | O31743 |
PRIDEi | O31743 |
Genome annotation databases
EnsemblBacteriai | CAB13478; CAB13478; BSU_16050 |
GeneIDi | 940134 |
KEGGi | bsu:BSU16050 |
PATRICi | fig|224308.179.peg.1745 |
Phylogenomic databases
eggNOGi | COG1161, Bacteria |
InParanoidi | O31743 |
OMAi | GVLWPKF |
PhylomeDBi | O31743 |
Enzyme and pathway databases
BioCyci | BSUB:BSU16050-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O31743 |
PROi | PR:O31743 |
Family and domain databases
Gene3Di | 1.10.1580.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR006073, GTP-bd IPR023179, GTP-bd_ortho_bundle_sf IPR019991, GTP-bd_ribosome_bgen IPR016478, GTPase_MTG1 IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit |
PIRSFi | PIRSF006230, MG442, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR03596, GTPase_YlqF, 1 hit |
PROSITEi | View protein in PROSITE PS51721, G_CP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBGA_BACSU | |
Accessioni | O31743Primary (citable) accession number: O31743 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | January 1, 1998 | |
Last modified: | September 29, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families