We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - O31665 (MTNE_BACSU)
Protein
L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferase
Gene
mtnE
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the methylthioribose (MTR) recycling pathway (PubMed:12022921, PubMed:24837359).
Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) (PubMed:12670965, PubMed:24837359).
3 PublicationsCatalytic activityi
- EC:2.6.1.1172 Publications
Cofactori
pyridoxal 5'-phosphateBy similarity
Kineticsi
kcat is 2.32 sec(-1).1 Publication
- KM=1.91 mM for KMTB1 Publication
: L-methionine biosynthesis via salvage pathway Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.2 Publications This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.
GO - Molecular functioni
- pyridoxal phosphate binding Source: InterPro
- transaminase activity Source: UniProtKB-KW
GO - Biological processi
- L-methionine salvage from methylthioadenosine Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | BSUB:BSU13580-MONOMER |
BRENDAi | 2.6.1.117, 658 |
UniPathwayi | UPA00904;UER00879 |
Names & Taxonomyi
Protein namesi | Recommended name: L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferaseCurated (EC:2.6.1.1172 Publications)Alternative name(s): GTK1 Publication Glutamine transaminase MtnECurated |
Gene namesi | Name:mtnE1 Publication Synonyms:mtnV1 Publication, ykrV Ordered Locus Names:BSU13580 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption mutant grows more slowly than the wild type strain on methylthioribose (MTR) as the sulfur source.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123924 | 1 – 398 | L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferaseAdd BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 240 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | O31665 |
PRIDEi | O31665 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily.Curated
Phylogenomic databases
eggNOGi | COG0436, Bacteria |
InParanoidi | O31665 |
OMAi | YPHMPTG |
PhylomeDBi | O31665 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
O31665-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFEQSHVLK ELPKQFFASL VQKVNRKLAE GHDVINLGQG NPDQPTPEHI
60 70 80 90 100
VEEMKRAVAD PENHKYSSFR GSYRLKSAAA AFYKREYGID LDPETEVAVL
110 120 130 140 150
FGGKAGLVEL PQCLLNPGDT ILVPDPGYPD YWSGVTLAKA KMEMMPLVKD
160 170 180 190 200
RAFLPDYSSI TAEIREQAKL MYLNYPNNPT GAVATSEFFE DTVRFAAENG
210 220 230 240 250
ICVVHDFAYG AVGFDGCKPL SFLQTEGAKD IGIEIYTLSK TYNMAGWRVG
260 270 280 290 300
FAVGNASVIE AINLYQDHMF VSLFRATQEA AAEALLADQT CVAEQNARYE
310 320 330 340 350
SRRNAWITAC REIGWDVTAP AGSFFAWLPV PEGYTSEQFS DLLLEKANVA
360 370 380 390
VAAGNGFGEY GEGYVRVGLL TSEERLKEAA YRIGKLNLFT QKSIDKTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13231.1 |
PIRi | F69863 |
RefSeqi | NP_389241.1, NC_000964.3 WP_003244774.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13231; CAB13231; BSU_13580 |
GeneIDi | 939330 |
KEGGi | bsu:BSU13580 |
PATRICi | fig|224308.179.peg.1475 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13231.1 |
PIRi | F69863 |
RefSeqi | NP_389241.1, NC_000964.3 WP_003244774.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | O31665 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU13580 |
Proteomic databases
PaxDbi | O31665 |
PRIDEi | O31665 |
Genome annotation databases
EnsemblBacteriai | CAB13231; CAB13231; BSU_13580 |
GeneIDi | 939330 |
KEGGi | bsu:BSU13580 |
PATRICi | fig|224308.179.peg.1475 |
Phylogenomic databases
eggNOGi | COG0436, Bacteria |
InParanoidi | O31665 |
OMAi | YPHMPTG |
PhylomeDBi | O31665 |
Enzyme and pathway databases
UniPathwayi | UPA00904;UER00879 |
BioCyci | BSUB:BSU13580-MONOMER |
BRENDAi | 2.6.1.117, 658 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MTNE_BACSU | |
Accessioni | O31665Primary (citable) accession number: O31665 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families