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Entry version 130 (26 Feb 2020)
Sequence version 2 (05 May 2009)
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Protein

Transcriptional regulator ManR

Gene

manR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Positively regulates the expression of the mannose operon that consists of three genes, manP, manA, and yjdF, which are responsible for the transport and utilization of mannose. Also activates its own expression.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

The regulatory activity of ManR is modulated by phosphorylation and dephosphorylation of the various ManR domains. It becomes activated via phosphoryl group transfer from PEP, EI and HPr on the two conserved histidine residues in the PRD 2 domain, whereas phosphorylation of the EIIA-like domain on His-570 by the PTS EIIB-Man domain of ManP inactivates ManR (PubMed:23551403).1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding, Kinase, Transferase
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU12000-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcriptional regulator ManR
Alternative name(s):
Mannose operon transcriptional activator
Including the following 2 domains:
Putative phosphotransferase enzyme IIB component (EC:2.7.1.191)
Alternative name(s):
Putative PTS system EIIB component
Putative phosphotransferase enzyme IIA component
Alternative name(s):
Putative PTS system EIIA component
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:manR
Ordered Locus Names:BSU12000
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene are unable to grow with mannose as the sole carbon source.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi222H → A: Reduced DNA binding. 1 Publication1
Mutagenesisi281H → A: Reduced DNA binding. 1 Publication1
Mutagenesisi334H → A: Loss of DNA binding. 1 Publication1
Mutagenesisi393H → A: Loss of DNA binding. 1 Publication1
Mutagenesisi415C → A: No effect on DNA binding. Results in a weak constitutive ManR activity. 1 Publication1
Mutagenesisi570H → A: No reduction in DNA-binding activity can be observed in the presence of EIIBA-Man. Leads to a nearly constitutive ManR activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003760801 – 648Transcriptional regulator ManRAdd BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei222Phosphohistidine; by HPrPROSITE-ProRule annotation1
Modified residuei281Phosphohistidine; by HPrPROSITE-ProRule annotation1
Modified residuei334Phosphohistidine; by HPr1 Publication1
Modified residuei393Phosphohistidine; by HPr1 Publication1
Modified residuei415Phosphocysteine; by EIIA1 Publication1
Modified residuei570Phosphohistidine; by EIIB1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
O31644

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
O31644

PRoteomics IDEntifications database

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PRIDEi
O31644

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O31644

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated by mannose. Is subject to carbon catabolite repression (CCR) by glucose.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU12000

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O31644

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini187 – 292PRD 1PROSITE-ProRule annotationAdd BLAST106
Domaini297 – 404PRD 2PROSITE-ProRule annotationAdd BLAST108
Domaini409 – 500PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST92
Domaini510 – 648PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST139

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1762 LUCA
COG3711 LUCA

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O31644

KEGG Orthology (KO)

More...
KOi
K02538

Identification of Orthologs from Complete Genome Data

More...
OMAi
MVEVTKM

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O31644

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.10, 1 hit
3.40.930.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013196 HTH_11
IPR007737 Mga_HTH
IPR011608 PRD
IPR036634 PRD_sf
IPR016152 PTrfase/Anion_transptr
IPR002178 PTS_EIIA_type-2_dom
IPR036095 PTS_EIIB-like_sf
IPR013011 PTS_EIIB_2
IPR036388 WH-like_DNA-bd_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08279 HTH_11, 1 hit
PF05043 Mga, 1 hit
PF00874 PRD, 2 hits
PF00359 PTS_EIIA_2, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52794 SSF52794, 1 hit
SSF55804 SSF55804, 1 hit
SSF63520 SSF63520, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51372 PRD_2, 2 hits
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS00372 PTS_EIIA_TYPE_2_HIS, 1 hit
PS51099 PTS_EIIB_TYPE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O31644-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEYINTRQKE ILYLLLSEPD DYLVVQDFAD RVQCSEKTIR NDLKVIEDYL
60 70 80 90 100
NEHSHAQLIR KPGLGVYLHI EEQERTWLSQ QLHTEHFSSR QRSDKERMLH
110 120 130 140 150
IAYDLLMNPK PVSAKDIAAR HFVNRSSIKK DLYAVEEWLK RFDLTLVSRQ
160 170 180 190 200
RLGLKVEGNE RNKRKALARI SDLIHNTAFT SQFIKSKFLH YEVDFVTKEI
210 220 230 240 250
KSLQKKHSLY FTDETFESLL LHTLLMVRRI KMKQPISLSP KEMAAVKKKK
260 270 280 290 300
EYQWTFACLQ RLEPVFAIRF PEEEAVYLTL HILGGKVRYP LQTEENLENA
310 320 330 340 350
VLPKVVGHLI NRVSELKMMD FHKDQDLING LNIHLNTVLQ RLSYDLSVAN
360 370 380 390 400
PMLNDIKKMY PYLFHLIIDV LEDINQTFDL HIPEEEAAYL TLHFQAAIER
410 420 430 440 450
MQGSSETHKK AVIVCHMGIG MSQLLRTKIE RKYHQIAVMA CIAKADLKDY
460 470 480 490 500
IKKHEDIDLV ISTIALENIT VPHIVVSPLL EPGEEKKLSA FIRQLGESHR
510 520 530 540 550
QKQKTFQMLN NTTPFLVFLQ QEAEHRYKLI EQLATALFEK GYVDKDYAVH
560 570 580 590 600
AVMREKMSAT NIGSGIAIPH ANAKFIKQSA IAIATLKEPL EWGNEKVSLV
610 620 630 640
FMLAVKHEDQ TMTKQLFSEL SYLSEQPAFV QKLTKETNVM TFLSHLDY
Length:648
Mass (Da):75,351
Last modified:May 5, 2009 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE987358049EBA550
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13057.2

NCBI Reference Sequences

More...
RefSeqi
NP_389082.2, NC_000964.3
WP_003245617.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB13057; CAB13057; BSU12000

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
939394

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU12000

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.1295

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13057.2
RefSeqiNP_389082.2, NC_000964.3
WP_003245617.1, NZ_JNCM01000035.1

3D structure databases

SMRiO31644
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU12000

PTM databases

iPTMnetiO31644

Proteomic databases

jPOSTiO31644
PaxDbiO31644
PRIDEiO31644

Genome annotation databases

EnsemblBacteriaiCAB13057; CAB13057; BSU12000
GeneIDi939394
KEGGibsu:BSU12000
PATRICifig|224308.179.peg.1295

Phylogenomic databases

eggNOGiCOG1762 LUCA
COG3711 LUCA
InParanoidiO31644
KOiK02538
OMAiMVEVTKM
PhylomeDBiO31644

Enzyme and pathway databases

BioCyciBSUB:BSU12000-MONOMER

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.40.930.10, 1 hit
InterProiView protein in InterPro
IPR013196 HTH_11
IPR007737 Mga_HTH
IPR011608 PRD
IPR036634 PRD_sf
IPR016152 PTrfase/Anion_transptr
IPR002178 PTS_EIIA_type-2_dom
IPR036095 PTS_EIIB-like_sf
IPR013011 PTS_EIIB_2
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF08279 HTH_11, 1 hit
PF05043 Mga, 1 hit
PF00874 PRD, 2 hits
PF00359 PTS_EIIA_2, 1 hit
SUPFAMiSSF52794 SSF52794, 1 hit
SSF55804 SSF55804, 1 hit
SSF63520 SSF63520, 2 hits
PROSITEiView protein in PROSITE
PS51372 PRD_2, 2 hits
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS00372 PTS_EIIA_TYPE_2_HIS, 1 hit
PS51099 PTS_EIIB_TYPE_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMANR_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O31644
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 5, 2009
Last modified: February 26, 2020
This is version 130 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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