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UniProtKB - O31428 (SKFF_BACSU)
Protein
Putative bacteriocin-SkfA transport system permease protein SkfF
Gene
skfF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Probably part of the ABC transporter SkfEF involved in the export of the bacteriocin SKF. Probably responsible for the translocation of bacteriocin SkfA across the membrane.
1 PublicationMiscellaneous
Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.1 Publication
GO - Biological processi
- bacteriocin biosynthetic process Source: UniProtKB-KW
Keywordsi
Biological process | Antibiotic biosynthesis, Bacteriocin biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU01960-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.128.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Putative bacteriocin-SkfA transport system permease protein SkfF |
Gene namesi | Name:skfF1 Publication Synonyms:ybdB Ordered Locus Names:BSU01960 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 4 – 22 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 23 – 29 | ExtracellularSequence analysis | 7 | |
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 51 – 59 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 60 – 80 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 81 – 85 | ExtracellularSequence analysis | 5 | |
Transmembranei | 86 – 104 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 105 – 113 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 135 – 141 | ExtracellularSequence analysis | 7 | |
Transmembranei | 142 – 160 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 161 – 189 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 190 – 208 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 209 – 247 | ExtracellularSequence analysisAdd BLAST | 39 | |
Transmembranei | 248 – 268 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 269 – 297 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 341 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 342 – 360 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 361 – 363 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 364 – 382 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 383 – 404 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 405 – 423 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 424 – 426 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 427 – 447 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312741 | 1 – 447 | Putative bacteriocin-SkfA transport system permease protein SkfFAdd BLAST | 447 |
Proteomic databases
PaxDbi | O31428 |
Expressioni
Inductioni
By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.2 Publications
Structurei
Family & Domainsi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG503383K, Bacteria |
Family and domain databases
InterProi | View protein in InterPro IPR030920, SkfF |
TIGRFAMsi | TIGR04405, SkfF, 1 hit |
i Sequence
Sequence statusi: Complete.
O31428-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFLIMLLFV GAIGFQVSFV SRSTTWDMSI AGWVLTGVFI LYTAFGLFSN
60 70 80 90 100
RLPSQMADII WLYGTATSFS KVVYSVLFFS VTWKALLWII SAIFGDVLIV
110 120 130 140 150
LLSGDHINLL GRSIIFVGLF FIAEVWLMSV SCARTVKKMK RVYVLVFLLM
160 170 180 190 200
LGIYSICLYR FFFLQHSSGI WESIARFISG VGLVFDTLSP LYVVVFIGII
210 220 230 240 250
TVSFMTIAFT SRQVEMKESL VKEAEFWEEF QERQFGSGQI IQKPKTTWWG
260 270 280 290 300
LQGLNGIWSF LWLELLLFKK YLFFHSIHTV MLSGVFYVVI FMYPEWFYLL
310 320 330 340 350
FFLIVSAVML SSYYSGIVRH SQSGTLHLFP GALWKKIIIL ELTNTVWLYI
360 370 380 390 400
LYCVSITFMA VGNLVYWYIY GLGIYIWFMT IRLFAFTHTN RNDIKLSLPQ
410 420 430 440
YYKSFFMALG LSGICLYVIH LLTADWYTLV VVVCIGSLSW CLFYRFR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 205 | M → I in BAA33093 (Ref. 1) Curated | 1 | |
Sequence conflicti | 216 | M → I in BAA33093 (Ref. 1) Curated | 1 | |
Sequence conflicti | 396 | L → LL in BAA33093 (Ref. 1) Curated | 1 | |
Sequence conflicti | 412 | S → T in BAA33093 (Ref. 1) Curated | 1 | |
Sequence conflicti | 447 | R → K in BAA33093 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006424 Genomic DNA Translation: BAA33093.1 AL009126 Genomic DNA Translation: CAB11990.2 |
PIRi | A69747 |
RefSeqi | NP_388078.2, NC_000964.3 WP_010886391.1, NC_000964.3 |
Genome annotation databases
EnsemblBacteriai | CAB11990; CAB11990; BSU_01960 |
GeneIDi | 938494 |
KEGGi | bsu:BSU01960 |
PATRICi | fig|224308.43.peg.198 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006424 Genomic DNA Translation: BAA33093.1 AL009126 Genomic DNA Translation: CAB11990.2 |
PIRi | A69747 |
RefSeqi | NP_388078.2, NC_000964.3 WP_010886391.1, NC_000964.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 224308.BSU01960 |
Protein family/group databases
TCDBi | 3.A.1.128.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | O31428 |
Genome annotation databases
EnsemblBacteriai | CAB11990; CAB11990; BSU_01960 |
GeneIDi | 938494 |
KEGGi | bsu:BSU01960 |
PATRICi | fig|224308.43.peg.198 |
Phylogenomic databases
eggNOGi | ENOG503383K, Bacteria |
Enzyme and pathway databases
BioCyci | BSUB:BSU01960-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR030920, SkfF |
TIGRFAMsi | TIGR04405, SkfF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SKFF_BACSU | |
Accessioni | O31428Primary (citable) accession number: O31428 Secondary accession number(s): Q7DL61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 83 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |