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UniProtKB - O30124 (NPD2_ARCFU)
Protein
NAD-dependent protein deacylase 2
Gene
cobB2
Organism
Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)
Status
Functioni
NAD-dependent protein deacetylase which modulates the activities of several proteins which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.
UniRule annotation1 PublicationMiscellaneous
The two SIR2 homologs in this organism, Af1 and Af2, display different substrate specificities in vitro. Af2 can deacetylate histones and peptides corresponding to the C-terminal part of p53 in a NAD-dependent manner (PubMed:12408821).1 Publication
Catalytic activityi
- H2O + N6-acetyl-L-lysyl-[protein] + NAD+ = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamideUniRule annotationEC:2.3.1.286UniRule annotation
Cofactori
Zn2+UniRule annotation3 PublicationsNote: Binds 1 zinc ion per subunit.UniRule annotation3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 118 | Proton acceptor | 1 | |
Metal bindingi | 126 | ZincUniRule annotation2 Publications | 1 | |
Metal bindingi | 129 | ZincUniRule annotation2 Publications | 1 | |
Metal bindingi | 150 | ZincUniRule annotation2 Publications | 1 | |
Metal bindingi | 153 | ZincUniRule annotation2 Publications | 1 | |
Binding sitei | 235 | NAD; via amide nitrogenUniRule annotation2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 23 – 42 | NADUniRule annotation2 PublicationsAdd BLAST | 20 | |
Nucleotide bindingi | 100 – 103 | NADUniRule annotation2 Publications | 4 | |
Nucleotide bindingi | 191 – 193 | NADUniRule annotation2 Publications | 3 | |
Nucleotide bindingi | 217 – 219 | NADUniRule annotation2 Publications | 3 |
GO - Molecular functioni
- NAD+ binding Source: UniProtKB-UniRule
- NAD-dependent protein deacetylase activity Source: UniProtKB-UniRule
- protein-malonyllysine demalonylase activity Source: InterPro
- protein-succinyllysine desuccinylase activity Source: InterPro
- transferase activity Source: UniProtKB-KW
- zinc ion binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
BRENDAi | 2.3.1.B41, 414 |
Names & Taxonomyi
Protein namesi | Recommended name: NAD-dependent protein deacylase 2UniRule annotation (EC:2.3.1.286UniRule annotation)Alternative name(s): Regulatory protein SIR2 homolog 2UniRule annotation SIR2-Af2 |
Gene namesi | Name:cobB2UniRule annotation Synonyms:Sir2Af2 Ordered Locus Names:AF_0112 |
Organismi | Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) |
Taxonomic identifieri | 224325 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110378 | 1 – 253 | NAD-dependent protein deacylase 2Add BLAST | 253 |
Proteomic databases
PRIDEi | O30124 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O30124 |
SMRi | O30124 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O30124 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 251 | Deacetylase sirtuin-typeUniRule annotationAdd BLAST | 246 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG04248, Archaea |
HOGENOMi | CLU_023643_3_1_2 |
OMAi | ERDCHGL |
OrthoDBi | 82426at2157 |
PhylomeDBi | O30124 |
Family and domain databases
Gene3Di | 3.30.1600.10, 1 hit |
HAMAPi | MF_01121, Sirtuin_ClassIII, 1 hit MF_01968, Sirtuin_ClassU, 1 hit |
IDEALi | IID90001 |
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR003000, Sirtuin IPR026591, Sirtuin_cat_small_dom_sf IPR027546, Sirtuin_class_III IPR028628, Sirtuin_class_U IPR026590, Ssirtuin_cat_dom |
Pfami | View protein in Pfam PF02146, SIR2, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit |
PROSITEi | View protein in PROSITE PS50305, SIRTUIN, 1 hit |
i Sequence
Sequence statusi: Complete.
O30124-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDEIRKAAE ILAKSKHAVV FTGAGISAES GIPTFRGEDG LWRKYDPEEV
60 70 80 90 100
ASISGFKRNP RAFWEFSMEM KDKLFAEPNP AHYAIAELER MGIVKAVITQ
110 120 130 140 150
NIDMLHQRAG SRRVLELHGS MDKLDCLDCH ETYDWSEFVE DFNKGEIPRC
160 170 180 190 200
RKCGSYYVKP RVVLFGEPLP QRTLFEAIEE AKHCDAFMVV GSSLVVYPAA
210 220 230 240 250
ELPYIAKKAG AKMIIVNAEP TMADPIFDVK IIGKAGEVLP KIVEEVKRLR
SEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000782 Genomic DNA Translation: AAB91115.1 |
PIRi | H69263 |
RefSeqi | WP_010877626.1, NC_000917.1 |
Genome annotation databases
EnsemblBacteriai | AAB91115; AAB91115; AF_0112 |
GeneIDi | 24793666 |
KEGGi | afu:AF_0112 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000782 Genomic DNA Translation: AAB91115.1 |
PIRi | H69263 |
RefSeqi | WP_010877626.1, NC_000917.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MA3 | X-ray | 2.00 | A | 1-253 | [»] | |
1S7G | X-ray | 2.30 | A/B/C/D/E | 1-253 | [»] | |
1YC2 | X-ray | 2.40 | A/B/C/D/E | 1-253 | [»] | |
4TWJ | X-ray | 1.65 | A | 1-253 | [»] | |
AlphaFoldDBi | O30124 | |||||
SMRi | O30124 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224325.AF_0112 |
Proteomic databases
PRIDEi | O30124 |
Genome annotation databases
EnsemblBacteriai | AAB91115; AAB91115; AF_0112 |
GeneIDi | 24793666 |
KEGGi | afu:AF_0112 |
Phylogenomic databases
eggNOGi | arCOG04248, Archaea |
HOGENOMi | CLU_023643_3_1_2 |
OMAi | ERDCHGL |
OrthoDBi | 82426at2157 |
PhylomeDBi | O30124 |
Enzyme and pathway databases
BRENDAi | 2.3.1.B41, 414 |
Miscellaneous databases
EvolutionaryTracei | O30124 |
Family and domain databases
Gene3Di | 3.30.1600.10, 1 hit |
HAMAPi | MF_01121, Sirtuin_ClassIII, 1 hit MF_01968, Sirtuin_ClassU, 1 hit |
IDEALi | IID90001 |
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR003000, Sirtuin IPR026591, Sirtuin_cat_small_dom_sf IPR027546, Sirtuin_class_III IPR028628, Sirtuin_class_U IPR026590, Ssirtuin_cat_dom |
Pfami | View protein in Pfam PF02146, SIR2, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit |
PROSITEi | View protein in PROSITE PS50305, SIRTUIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NPD2_ARCFU | |
Accessioni | O30124Primary (citable) accession number: O30124 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2003 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families