Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable copper-exporting P-type ATPase

Gene

copA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably involved in copper and silver export.1 Publication

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).1 Publication

Activity regulationi

Activated by Cu+ and Ag+ and inhibited by vanadate. Activated by CopZ in its Cu+-bound form.2 Publications

Kineticsi

Shows higher affinity for Cu+ compared with Ag+.
  1. KM=0.25 mM for ATP2 Publications
  1. Vmax=14.9 µmol/h/mg enzyme with Ag+ as substrate2 Publications
  2. Vmax=3.7 µmol/h/mg enzyme with Cu+ as substrate2 Publications

pH dependencei

Optimum pH is 6.1-6.5.2 Publications

Temperature dependencei

Optimum temperature is 75 degrees Celsius.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27CopperPROSITE-ProRule annotation1
Metal bindingi30CopperPROSITE-ProRule annotation1
Active sitei4244-aspartylphosphate intermediateBy similarity1
Metal bindingi618MagnesiumPROSITE-ProRule annotation1
Metal bindingi622MagnesiumPROSITE-ProRule annotation1
Metal bindingi751CopperPROSITE-ProRule annotation1
Metal bindingi754CopperPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi457 – 462ATPSequence analysis6
Nucleotide bindingi490 – 501ATPSequence analysisAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cation-transporting ATPase activity Source: InterPro
  • copper ion binding Source: InterPro
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCopper transport, Ion transport, Transport
LigandATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.54 414
SABIO-RKiO29777

Protein family/group databases

TCDBi3.A.3.5.7 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Probable copper-exporting P-type ATPase (EC:3.6.3.54)
Alternative name(s):
Copper-exporting P-type ATPase A
Cu(+)-exporting ATPase
Gene namesi
Name:copA
Synonyms:pacS
Ordered Locus Names:AF_0473
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 101CytoplasmicSequence analysisAdd BLAST101
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 128ExtracellularSequence analysis6
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 159CytoplasmicSequence analysis10
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 186ExtracellularSequence analysis6
Transmembranei187 – 204HelicalSequence analysisAdd BLAST18
Topological domaini205 – 339CytoplasmicSequence analysisAdd BLAST135
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Topological domaini361 – 364ExtracellularSequence analysis4
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 680CytoplasmicSequence analysisAdd BLAST295
Transmembranei681 – 701HelicalSequence analysisAdd BLAST21
Topological domaini702 – 704ExtracellularSequence analysis3
Transmembranei705 – 725HelicalSequence analysisAdd BLAST21
Topological domaini726 – 804CytoplasmicSequence analysisAdd BLAST79

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27C → A: Reduction in ATPase activity; when associated with A-30. 2 Publications1
Mutagenesisi30C → A: Reduction in ATPase activity; when associated with A-27. 2 Publications1
Mutagenesisi380C → A: Abolishes activity. 2 Publications1
Mutagenesisi382C → A or S: Abolishes activity. 2 Publications1
Mutagenesisi751C → A: No effect on ATPase activity; when associated with A-754. Reduction in ATPase activity; when associated with A-27; A-30 and A-754. 2 Publications1
Mutagenesisi754C → A: No effect on ATPase activity, when associated with A-751. Reduction in ATPase activity; when associated with A-27; A-30 and A-751. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003506011 – 804Probable copper-exporting P-type ATPaseAdd BLAST804

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO29777

Interactioni

Subunit structurei

Interacts with CopZ probably in the CopZ Cu+-bound form.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9016967,EBI-9016967

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46021N
STRINGi224325.AF0473

Structurei

Secondary structure

1804
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO29777
SMRiO29777
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO29777

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 83HMA 1PROSITE-ProRule annotationAdd BLAST67
Domaini741 – 802HMA 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG01576 Archaea
COG2217 LUCA
KOiK17686
OMAiHWMLPAW
OrthoDBiPOG093Z00O0

Family and domain databases

CDDicd00371 HMA, 2 hits
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006122 HMA_Cu_ion-bd
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 2 hits
SUPFAMiSSF55008 SSF55008, 2 hits
SSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
TIGR00003 TIGR00003, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 1 hit
PS50846 HMA_2, 2 hits

Sequencei

Sequence statusi: Complete.

O29777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKDTYISSA SKTPPMERTV RVTGMTCAMC VKSIETAVGS LEGVEEVRVN
60 70 80 90 100
LATETAFIRF DEKRIDFETI KRVIEDLGYG VVDEQAAVSA EVEHLSRMKR
110 120 130 140 150
KLYVAAFAGV LLLFLAHFIS LPYEDFVQLL IALPAIFYSG SSIFKAAFSA
160 170 180 190 200
LRRRTLNMDV MYSMGVGAAF LASVLSTAGV LPREYSFYET SVLLLAFLLL
210 220 230 240 250
GRTLEARAKS RTGEAIKKLV GLQAKTAVVI RDGKEIAVPV EEVAVGDIVI
260 270 280 290 300
VRPGEKIPVD GVVVEGESYV DESMISGEPV PVLKSKGDEV FGATINNTGV
310 320 330 340 350
LKIRATRVGG ETLLAQIVKL VEDAMGSKPP IQRLADKVVA YFIPTVLLVA
360 370 380 390 400
ISAFIYWYFI AHAPLLFAFT TLIAVLVVAC PCAFGLATPT ALTVGMGKGA
410 420 430 440 450
ELGILIKNAD ALEVAEKVTA VIFDKTGTLT KGKPEVTDLV PLNGDERELL
460 470 480 490 500
RLAAIAERRS EHPIAEAIVK KALEHGIELG EPEKVEVIAG EGVVADGILV
510 520 530 540 550
GNKRLMEDFG VAVSNEVELA LEKLEREAKT AVIVARNGRV EGIIAVSDTL
560 570 580 590 600
KESAKPAVQE LKRMGIKVGM ITGDNWRSAE AISRELNLDL VIAEVLPHQK
610 620 630 640 650
SEEVKKLQAK EVVAFVGDGI NDAPALAQAD LGIAVGSGSD VAVESGDIVL
660 670 680 690 700
IRDDLRDVVA AIQLSRKTMS KIKQNIFWAL IYNVILIPAA AGLLYPIFGV
710 720 730 740 750
VFRPEFAGLA MAMSSVSVVA NSLLLRNYVP PIRRGGDSVE KIVLELSGLS
760 770 780 790 800
CHHCVARVKK ALEEAGAKVE KVDLNEAVVA GNKEDVDKYI KAVEAAGYQA

KLRS
Length:804
Mass (Da):86,432
Last modified:January 1, 1998 - v1
Checksum:i610EB948C1D6B16F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB90763.1
PIRiA69309
RefSeqiWP_010877980.1, NC_000917.1

Genome annotation databases

EnsemblBacteriaiAAB90763; AAB90763; AF_0473
GeneIDi24794013
KEGGiafu:AF_0473

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB90763.1
PIRiA69309
RefSeqiWP_010877980.1, NC_000917.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B8EX-ray2.30A/B/C407-671[»]
2HC8X-ray1.65A214-325[»]
2VOYelectron microscopy18.00F214-326[»]
I410-663[»]
J432-549[»]
3A1CX-ray1.85A/B398-673[»]
3A1DX-ray1.85A/B398-673[»]
3A1EX-ray1.95A/B398-673[»]
3FRYX-ray2.00A/B736-804[»]
3J08electron microscopy-A/B93-737[»]
3J09electron microscopy-A/B15-737[»]
ProteinModelPortaliO29777
SMRiO29777
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46021N
STRINGi224325.AF0473

Protein family/group databases

TCDBi3.A.3.5.7 the p-type atpase (p-atpase) superfamily

Proteomic databases

PRIDEiO29777

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90763; AAB90763; AF_0473
GeneIDi24794013
KEGGiafu:AF_0473

Phylogenomic databases

eggNOGiarCOG01576 Archaea
COG2217 LUCA
KOiK17686
OMAiHWMLPAW
OrthoDBiPOG093Z00O0

Enzyme and pathway databases

BRENDAi3.6.3.54 414
SABIO-RKiO29777

Miscellaneous databases

EvolutionaryTraceiO29777

Family and domain databases

CDDicd00371 HMA, 2 hits
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006122 HMA_Cu_ion-bd
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 2 hits
SUPFAMiSSF55008 SSF55008, 2 hits
SSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
TIGR00003 TIGR00003, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 1 hit
PS50846 HMA_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCOPA_ARCFU
AccessioniPrimary (citable) accession number: O29777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: January 1, 1998
Last modified: September 12, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again