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Protein

Piwi protein AF_1318

Gene

AF_1318

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Might play a role in defense against invading genetic elements, using short DNA sequences as guides to bind complementary target strands, resulting in slicing of the target nucleic acid (By similarity). Binds nucleic acids with decreasing affinity in the following order; ssDNA, ssRNA, dsDNA, RNA-DNA, RNA-RNA (PubMed:15800629). Association of the 5' seed region of the guide strand (nucleotides 2-7) with AfPiwi increases affinity for the corresponding target strand; the greatest increase in affinity is for guide DNA with target RNA (PubMed:19187762).By similarity2 Publications

Miscellaneous

This is missing the N-terminal and PAZ domains compared to other prokaryotic argonaute proteins.Curated

Caution

Despite its similarity to eukaryotic argonaute proteins this protein is probably not involved in RNA-mediated gene silencing (RNAi), as that process is not known to exist in prokaryotes.Curated

Cofactori

a divalent metal cation3 PublicationsNote: Other ligands are provided by nucleic acid (PubMed:15800628, PubMed:15800629, PubMed:19187762).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159Divalent metal cationCombined sources1
Metal bindingi427Divalent metal cation; via amide nitrogen and carbonyl oxygenCombined sources1

GO - Molecular functioni

Keywordsi

Molecular functionDNA-binding, RNA-binding
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Piwi protein AF_1318
Alternative name(s):
AfAgoCurated
AfPiwi1 Publication
PIWI/MID domain protein1 Publication
Gene namesi
Ordered Locus Names:AF_1318
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi123Y → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi127K → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi137Q → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi163K → A: Reduced binding affinity for siRNA. 1 Publication1
Mutagenesisi427L → LG: Reduced binding to siRNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003781461 – 427Piwi protein AF_1318Add BLAST427

Interactioni

Protein-protein interaction databases

IntActiO28951, 1 interactor
STRINGi224325.AF1318

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi12 – 14Combined sources3
Helixi16 – 18Combined sources3
Beta strandi20 – 24Combined sources5
Helixi26 – 36Combined sources11
Beta strandi45 – 54Combined sources10
Helixi59 – 74Combined sources16
Beta strandi82 – 87Combined sources6
Beta strandi89 – 92Combined sources4
Helixi93 – 106Combined sources14
Beta strandi109 – 117Combined sources9
Helixi120 – 131Combined sources12
Beta strandi136 – 140Combined sources5
Helixi141 – 143Combined sources3
Turni146 – 148Combined sources3
Helixi149 – 163Combined sources15
Helixi175 – 177Combined sources3
Beta strandi180 – 202Combined sources23
Beta strandi208 – 213Combined sources6
Helixi219 – 221Combined sources3
Helixi222 – 240Combined sources19
Beta strandi247 – 256Combined sources10
Helixi261 – 277Combined sources17
Beta strandi286 – 294Combined sources9
Beta strandi298 – 301Combined sources4
Turni304 – 307Combined sources4
Beta strandi313 – 328Combined sources16
Beta strandi332 – 334Combined sources3
Beta strandi340 – 353Combined sources14
Turni357 – 359Combined sources3
Helixi360 – 375Combined sources16
Beta strandi381 – 383Combined sources3
Helixi389 – 406Combined sources18
Turni414 – 416Combined sources3
Helixi419 – 422Combined sources4

3D structure databases

ProteinModelPortaliO28951
SMRiO28951
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28951

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 406PiwiPROSITE-ProRule annotationAdd BLAST297

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 124Binds 5'-phosphorylated end of guide DNACombined sources1 Publication7
Regioni147 – 148Binds target DNACombined sources1 Publication2
Regioni150 – 155Binds guide DNACombined sources1 Publication6

Sequence similaritiesi

Belongs to the argonaute family. Short pAgo subfamily.Curated

Phylogenomic databases

eggNOGiarCOG03890 Archaea
COG1431 LUCA
KOiK07578
OrthoDBiPOG093Z022N

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM00950 Piwi, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50822 PIWI, 1 hit

Sequencei

Sequence statusi: Complete.

O28951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEYKIVENG LTYRIGNGAS VPISNTGELI KGLRNYGPYE VPSLKYNQIA
60 70 80 90 100
LIHNNQFSSL INQLKSQISS KIDEVWHIHN INISEFIYDS PHFDSIKSQV
110 120 130 140 150
DNAIDTGVDG IMLVLPEYNT PLYYKLKSYL INSIPSQFMR YDILSNRNLT
160 170 180 190 200
FYVDNLLVQF VSKLGGKPWI LNVDPEKGSD IIIGTGATRI DNVNLFCFAM
210 220 230 240 250
VFKKDGTMLW NEISPIVTSS EYLTYLKSTI KKVVYGFKKS NPDWDVEKLT
260 270 280 290 300
LHVSGKRPKM KDGETKILKE TVEELKKQEM VSRDVKYAIL HLNETHPFWV
310 320 330 340 350
MGDPNNRFHP YEGTKVKLSS KRYLLTLLQP YLKRNGLEMV TPIKPLSVEI
360 370 380 390 400
VSDNWTSEEY YHNVHEILDE IYYLSKMNWR GFRSRNLPVT VNYPKLVAGI
410 420
IANVNRYGGY PINPEGNRSL QTNPWFL
Length:427
Mass (Da):49,244
Last modified:January 1, 1998 - v1
Checksum:iF925081F3AFB47CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB89939.1
PIRiE69414

Genome annotation databases

EnsemblBacteriaiAAB89939; AAB89939; AF_1318
KEGGiafu:AF_1318

Similar proteinsi

Entry informationi

Entry nameiPIWI_ARCFU
AccessioniPrimary (citable) accession number: O28951
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 1, 1998
Last modified: July 18, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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