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Protein

CCA-adding enzyme

Gene

cca

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.1 Publication

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition. In contrast to the eubacterial homologs, the specificity of the nucleotide-binding pocket is determined by both the enzyme and the tRNA.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei47ATP or CTP1
Binding sitei50ATP or CTP1
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi59Magnesium1
Metal bindingi61Magnesium1
Metal bindingi110Magnesium1
Binding sitei133ATP or CTP1
Binding sitei152ATP or CTP1
Binding sitei161ATP or CTP1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
Biological processRNA repair, tRNA processing
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.7.7.72 414

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
CCA-adding enzyme (EC:2.7.7.72)
Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:cca
Ordered Locus Names:AF_2156
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224325 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002199 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi50R → A: High decrease in both AMP and CMP incorporation. 1 Publication1
Mutagenesisi110D → A: High decrease in both AMP and CMP incorporation. 1 Publication1
Mutagenesisi133H → A: No decrease in both AMP and CMP incorporation. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001390651 – 437CCA-adding enzymeAdd BLAST437

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
O28126

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224325.AF2156

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R89X-ray1.80A1-437[»]
1R8AX-ray2.10A1-437[»]
1R8BX-ray2.00A1-437[»]
1R8CX-ray1.90A1-437[»]
1SZ1X-ray6.21A/B1-437[»]
1TFWX-ray2.20A/B/C/D1-437[»]
1TFYX-ray3.20A/B/C/D1-437[»]
1UETX-ray2.00A1-437[»]
1UEUX-ray2.00A1-437[»]
1UEVX-ray2.70A1-437[»]
2DR5X-ray2.80A1-437[»]
2DR7X-ray2.80A1-437[»]
2DR8X-ray2.50A1-437[»]
2DR9X-ray2.80A1-437[»]
2DRAX-ray2.50A1-437[»]
2DRBX-ray2.80A1-437[»]
2DVIX-ray2.61A1-437[»]
2ZH1X-ray2.80A1-437[»]
2ZH2X-ray2.66A1-437[»]
2ZH3X-ray2.50A1-437[»]
2ZH4X-ray2.65A1-437[»]
2ZH5X-ray2.60A1-437[»]
2ZH6X-ray2.50A1-437[»]
2ZH7X-ray3.00A2-437[»]
2ZH8X-ray2.65A1-437[»]
2ZH9X-ray2.90A1-437[»]
2ZHAX-ray2.95A1-437[»]
2ZHBX-ray3.05A2-437[»]
3OUYX-ray2.69A/B1-437[»]
3OV7X-ray3.00A/B1-437[»]
3OVAX-ray1.98A3-437[»]
3OVBX-ray1.95A/B1-437[»]
3OVSX-ray2.80A/B1-437[»]
4X4NX-ray2.95A/C/E/F1-437[»]
4X4OX-ray3.20A/C1-437[»]
4X4PX-ray3.00A/C/E/G1-437[»]
4X4QX-ray3.15A/C1-437[»]
4X4RX-ray3.20A/C1-437[»]
4X4SX-ray3.25A/C1-437[»]
4X4TX-ray2.50A/C/E/F1-437[»]
4X4UX-ray2.70A/C/E/F1-437[»]
4X4VX-ray2.60A/C1-437[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
O28126

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O28126

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
O28126

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG04249 Archaea
COG1746 LUCA

KEGG Orthology (KO)

More...
KOi
K07558

Identification of Orthologs from Complete Genome Data

More...
OMAi
VDIVPCY

Database of Orthologous Groups

More...
OrthoDBi
82755at2157

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_01264 CCA_arch, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008229 CCA-adding_arc
IPR011068 NuclTrfase_I-like_C
IPR002934 Polymerase_NTP_transf_dom
IPR015329 tRNA_NucTransf2

The PANTHER Classification System

More...
PANTHERi
PTHR39643 PTHR39643, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF09249 tRNA_NucTransf2, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005335 CCA_arch, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55003 SSF55003, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03671 cca_archaeal, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O28126-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVEEILEKA LELVIPDEEE VRKGREAEEE LRRRLDELGV EYVFVGSYAR
60 70 80 90 100
NTWLKGSLEI DVFLLFPEEF SKEELRERGL EIGKAVLDSY EIRYAEHPYV
110 120 130 140 150
HGVVKGVEVD VVPCYKLKEP KNIKSAVDRT PFHHKWLEGR IKGKENEVRL
160 170 180 190 200
LKGFLKANGI YGAEYKVRGF SGYLCELLIV FYGSFLETVK NARRWTRRTV
210 220 230 240 250
IDVAKGEVRK GEEFFVVDPV DEKRNVAANL SLDNLARFVH LCREFMEAPS
260 270 280 290 300
LGFFKPKHPL EIEPERLRKI VEERGTAVFA VKFRKPDIVD DNLYPQLERA
310 320 330 340 350
SRKIFEFLER ENFMPLRSAF KASEEFCYLL FECQIKEISR VFRRMGPQFE
360 370 380 390 400
DERNVKKFLS RNRAFRPFIE NGRWWAFEMR KFTTPEEGVR SYASTHWHTL
410 420 430
GKNVGESIRE YFEIISGEKL FKEPVTAELC EMMGVKD
Length:437
Mass (Da):51,385
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC5D57122F63CAC97
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB89084.1

Protein sequence database of the Protein Information Resource

More...
PIRi
D69519

NCBI Reference Sequences

More...
RefSeqi
WP_010879645.1, NC_000917.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAB89084; AAB89084; AF_2156

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
24795903

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
afu:AF_2156

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA Translation: AAB89084.1
PIRiD69519
RefSeqiWP_010879645.1, NC_000917.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R89X-ray1.80A1-437[»]
1R8AX-ray2.10A1-437[»]
1R8BX-ray2.00A1-437[»]
1R8CX-ray1.90A1-437[»]
1SZ1X-ray6.21A/B1-437[»]
1TFWX-ray2.20A/B/C/D1-437[»]
1TFYX-ray3.20A/B/C/D1-437[»]
1UETX-ray2.00A1-437[»]
1UEUX-ray2.00A1-437[»]
1UEVX-ray2.70A1-437[»]
2DR5X-ray2.80A1-437[»]
2DR7X-ray2.80A1-437[»]
2DR8X-ray2.50A1-437[»]
2DR9X-ray2.80A1-437[»]
2DRAX-ray2.50A1-437[»]
2DRBX-ray2.80A1-437[»]
2DVIX-ray2.61A1-437[»]
2ZH1X-ray2.80A1-437[»]
2ZH2X-ray2.66A1-437[»]
2ZH3X-ray2.50A1-437[»]
2ZH4X-ray2.65A1-437[»]
2ZH5X-ray2.60A1-437[»]
2ZH6X-ray2.50A1-437[»]
2ZH7X-ray3.00A2-437[»]
2ZH8X-ray2.65A1-437[»]
2ZH9X-ray2.90A1-437[»]
2ZHAX-ray2.95A1-437[»]
2ZHBX-ray3.05A2-437[»]
3OUYX-ray2.69A/B1-437[»]
3OV7X-ray3.00A/B1-437[»]
3OVAX-ray1.98A3-437[»]
3OVBX-ray1.95A/B1-437[»]
3OVSX-ray2.80A/B1-437[»]
4X4NX-ray2.95A/C/E/F1-437[»]
4X4OX-ray3.20A/C1-437[»]
4X4PX-ray3.00A/C/E/G1-437[»]
4X4QX-ray3.15A/C1-437[»]
4X4RX-ray3.20A/C1-437[»]
4X4SX-ray3.25A/C1-437[»]
4X4TX-ray2.50A/C/E/F1-437[»]
4X4UX-ray2.70A/C/E/F1-437[»]
4X4VX-ray2.60A/C1-437[»]
ProteinModelPortaliO28126
SMRiO28126
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2156

Proteomic databases

PRIDEiO28126

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89084; AAB89084; AF_2156
GeneIDi24795903
KEGGiafu:AF_2156

Phylogenomic databases

eggNOGiarCOG04249 Archaea
COG1746 LUCA
KOiK07558
OMAiVDIVPCY
OrthoDBi82755at2157

Enzyme and pathway databases

BRENDAi2.7.7.72 414

Miscellaneous databases

EvolutionaryTraceiO28126

Family and domain databases

HAMAPiMF_01264 CCA_arch, 1 hit
InterProiView protein in InterPro
IPR008229 CCA-adding_arc
IPR011068 NuclTrfase_I-like_C
IPR002934 Polymerase_NTP_transf_dom
IPR015329 tRNA_NucTransf2
PANTHERiPTHR39643 PTHR39643, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF09249 tRNA_NucTransf2, 1 hit
PIRSFiPIRSF005335 CCA_arch, 1 hit
SUPFAMiSSF55003 SSF55003, 1 hit
TIGRFAMsiTIGR03671 cca_archaeal, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCCA_ARCFU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O28126
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: January 16, 2019
This is version 123 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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