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Protein

Calcium-gated potassium channel MthK

Gene

mthK

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-gated potassium channel.

Miscellaneous

It is not known whether calcium is the physiological ligand.
Inhibited by charybdotoxin (CTX), a protein from scorpion venom.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi184Calcium1
Metal bindingi210Calcium1
Metal bindingi212Calcium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Potassium transport, Transport
LigandCalcium, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1472-MONOMER

Protein family/group databases

TCDBi1.A.1.13.2 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-gated potassium channel MthK
Gene namesi
Name:mthK
Ordered Locus Names:MTH_1520
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicAdd BLAST20
Transmembranei21 – 41Helical; Name=Outer helix M1Add BLAST21
Topological domaini42 – 48Extracellular7
Intramembranei49 – 58Helical; Pore-formingSequence analysis10
Intramembranei59 – 64Pore-formingSequence analysis6
Topological domaini65 – 69Extracellular5
Transmembranei70 – 95Helical; Name=Inner helix M2Add BLAST26
Topological domaini96 – 106CytoplasmicAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107M → I: Elimination of the 26 kDa product and reduced levels of channel expression. 1 Publication1
Mutagenesisi184D → N: At high calcium concentration, mean open time is short and mean closed time is long compared with wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000357771 – 336Calcium-gated potassium channel MthKAdd BLAST336

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1101987,EBI-1101987

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-37842N
STRINGi187420.MTH1520

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO27564
SMRiO27564
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO27564

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 237RCK N-terminalPROSITE-ProRule annotationAdd BLAST121
Domaini252 – 336RCK C-terminalPROSITE-ProRule annotationAdd BLAST85

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 64Selectivity filter6

Domaini

The channel is composed of 4 repeated units, each containing a transmembrane pore part and a gating ring part. The gating ring is composed of eight identical RCK (Regulators of K conductance) domains, in an alternating arrangement of one domain from each of the four subunits and four from the intracellular solution. Two protein interfaces between dimers of RCK domains from the pore-forming subunit and from the intracellular solution hold the ring together. One is called the fixed interface and the other the flexible interface. The flexible interface forms a cleft where calcium binds. Upon calcium binding the gating ring undergoes a conformational change that enables it to pull open the inner helices of the pore, allowing ion conduction.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG01958 Archaea
COG1226 LUCA
KOiK10716
OMAiVFEGIWW
OrthoDBiPOG093Z0554

Family and domain databases

Gene3Di3.30.70.1450, 1 hit
InterProiView protein in InterPro
IPR013099 K_chnl_dom
IPR036291 NAD(P)-bd_dom_sf
IPR006037 RCK_C
IPR036721 RCK_C_sf
IPR003148 RCK_N
PfamiView protein in Pfam
PF07885 Ion_trans_2, 1 hit
PF02080 TrkA_C, 1 hit
PF02254 TrkA_N, 1 hit
SUPFAMiSSF116726 SSF116726, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS51202 RCK_C, 1 hit
PS51201 RCK_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform 1 (identifier: O27564-1) [UniParc]FASTAAdd to basket
Also known as: Calcium-gated potassium channel

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVLVIEIIRK HLPRVLKVPA TRILLLVLAV IIYGTAGFHF IEGESWTVSL
60 70 80 90 100
YWTFVTIATV GYGDYSPSTP LGMYFTVTLI VLGIGTFAVA VERLLEFLIN
110 120 130 140 150
REQMKLMGLI DVAKSRHVVI CGWSESTLEC LRELRGSEVF VLAEDENVRK
160 170 180 190 200
KVLRSGANFV HGDPTRVSDL EKANVRGARA VIVDLESDSE TIHCILGIRK
210 220 230 240 250
IDESVRIIAE AERYENIEQL RMAGADQVIS PFVISGRLMS RSIDDGYEAM
260 270 280 290 300
FVQDVLAEES TRRMVEVPIP EGSKLEGVSV LDADIHDVTG VIIIGVGRGD
310 320 330
ELIIDPPRDY SFRAGDIILG IGKPEEIERL KNYISA
Length:336
Mass (Da):37,314
Last modified:January 1, 1998 - v1
Checksum:i31FDC5811CB79253
GO
Isoform Soluble (identifier: O27564-2) [UniParc]FASTAAdd to basket
Also known as: RCK domain

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.

Note: No experimental confirmation available.
Show »
Length:230
Mass (Da):25,416
Checksum:i90462DA6E2BC1649
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189011 – 106Missing in isoform Soluble. CuratedAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB85995.1
PIRiH69069
RefSeqiWP_010877130.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB85995; AAB85995; MTH_1520
GeneIDi1471789
KEGGimth:MTH_1520
PATRICifig|187420.15.peg.1483

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB85995.1
PIRiH69069
RefSeqiWP_010877130.1, NC_000916.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LNQX-ray3.30A/B/C/D/E/F/G/H1-336[»]
2AEFX-ray1.70A/B107-336[»]
2AEJX-ray2.10A/B107-336[»]
2AEMX-ray2.80A107-336[»]
2FY8X-ray2.79A/B/C/D/E/F/G/H107-336[»]
2OGUX-ray3.23A107-336[»]
3KXDX-ray2.20A/B116-336[»]
3LDCX-ray1.45A18-99[»]
3LDDX-ray1.45A18-99[»]
3LDEX-ray2.21A18-99[»]
3OUSX-ray1.75A18-99[»]
3R65X-ray1.80A18-99[»]
3RBXX-ray2.80A/B/C/D/E/F107-336[»]
3RBZX-ray3.40A/B/C/D1-336[»]
4EI2X-ray3.11A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P107-336[»]
4HYOX-ray1.65A/B/C/D/E/F/G/H11-101[»]
4HZ3X-ray1.70A/B/C/D/E/F/G/H11-101[»]
4L73X-ray2.50A/B107-336[»]
4L74X-ray1.84A/B107-336[»]
4L75X-ray2.39A/B/C/D/E/F107-336[»]
4L76X-ray2.99A/B/C/D/E/F107-336[»]
4QE7X-ray2.40A19-99[»]
4QE9X-ray2.15A18-100[»]
4RO0X-ray3.18A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/a/b/c/d/e/f/g/h/i/j107-336[»]
ProteinModelPortaliO27564
SMRiO27564
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-37842N
STRINGi187420.MTH1520

Protein family/group databases

TCDBi1.A.1.13.2 the voltage-gated ion channel (vic) superfamily

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85995; AAB85995; MTH_1520
GeneIDi1471789
KEGGimth:MTH_1520
PATRICifig|187420.15.peg.1483

Phylogenomic databases

eggNOGiarCOG01958 Archaea
COG1226 LUCA
KOiK10716
OMAiVFEGIWW
OrthoDBiPOG093Z0554

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1472-MONOMER

Miscellaneous databases

EvolutionaryTraceiO27564

Family and domain databases

Gene3Di3.30.70.1450, 1 hit
InterProiView protein in InterPro
IPR013099 K_chnl_dom
IPR036291 NAD(P)-bd_dom_sf
IPR006037 RCK_C
IPR036721 RCK_C_sf
IPR003148 RCK_N
PfamiView protein in Pfam
PF07885 Ion_trans_2, 1 hit
PF02080 TrkA_C, 1 hit
PF02254 TrkA_N, 1 hit
SUPFAMiSSF116726 SSF116726, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS51202 RCK_C, 1 hit
PS51201 RCK_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTHK_METTH
AccessioniPrimary (citable) accession number: O27564
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: January 1, 1998
Last modified: October 10, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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