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Entry version 152 (02 Jun 2021)
Sequence version 1 (01 Jan 1998)
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Protein

Proline--tRNA ligase

Gene

proS

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.26 mM for proline (at 60 degrees Celsius)2 Publications
  2. KM=0.05 mM for cysteine (at 60 degrees Celsius)2 Publications
  3. KM=4.1 µM for tRNA(Pro) (at 50 degrees Celsius)2 Publications
  4. KM=2.2 µM for tRNA(Pro) (at 50 degrees Celsius in the presence of LeuRS)2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei117Proline1 Publication1
    Binding sitei119Proline1 Publication1
    Binding sitei148ATP1 Publication1
    Binding sitei148Proline1 Publication1
    Binding sitei150ATP1 Publication1
    Binding sitei232ATP1 Publication1
    Binding sitei235ATP1 Publication1
    Binding sitei237Proline1 Publication1
    Binding sitei269ATP1 Publication1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi436Zinc; structural1 Publication1
    Metal bindingi441Zinc; structural1 Publication1
    Metal bindingi464Zinc; structural1 Publication1
    Metal bindingi467Zinc; structural1 Publication1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    O26708

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotationCurated (EC:6.1.1.15UniRule annotation1 Publication)
    Alternative name(s):
    Prolyl-tRNA synthetase1 PublicationUniRule annotation
    Short name:
    ProRS1 PublicationUniRule annotation
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:proS
    Ordered Locus Names:MTH_611
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri187420 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000005223 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001393521 – 482Proline--tRNA ligaseAdd BLAST482

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer. The dimer is functionally asymmetric: only one of the two active sites at a time is able to form prolyl-adenylate, and only one tRNA molecule binds per dimer.

    Interacts with LeuRS, which enhances tRNA(Pro) aminoacylation.

    3 Publications

    <p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

    Protein-protein interaction databases

    Protein interaction database and analysis system

    More...
    IntActi
    O26708, 1 interactor

    Molecular INTeraction database

    More...
    MINTi
    O26708

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1482
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O26708

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...
    EvolutionaryTracei
    O26708

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni346 – 376Interaction with tRNABy similarityAdd BLAST31

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension. The C-terminal extension binds a zinc ion, which probably plays a non-essential structural role in stabilizing the fold of C-terminal domain.

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotationCurated

    Phylogenomic databases

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_001882_4_2_2

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    EVYWVTH

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00778, ProRS_core_arch_euk, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.30.110.30, 1 hit
    3.40.50.800, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_01571, Pro_tRNA_synth_type3, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR002314, aa-tRNA-synt_IIb
    IPR006195, aa-tRNA-synth_II
    IPR004154, Anticodon-bd
    IPR036621, Anticodon-bd_dom_sf
    IPR002316, Pro-tRNA-ligase_IIa
    IPR004499, Pro-tRNA-ligase_IIa_arc-type
    IPR016061, Pro-tRNA_ligase_II_C
    IPR017449, Pro-tRNA_synth_II
    IPR033721, ProRS_core_arch_euk

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR43382, PTHR43382, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF03129, HGTP_anticodon, 1 hit
    PF09180, ProRS-C_1, 1 hit
    PF00587, tRNA-synt_2b, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR01046, TRNASYNTHPRO

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00946, ProRS-C_1, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF64586, SSF64586, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00408, proS_fam_I, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50862, AA_TRNA_LIGASE_II, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    O26708-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQKPIKKDPN RYHGEKMTEF SEWFHNILEE AEIIDQRYPV KGMHVWMPHG
    60 70 80 90 100
    FMIRKNTLKI LRRILDRDHE EVLFPLLVPE DELAKEAIHV KGFEDEVYWV
    110 120 130 140 150
    THGGLSKLQR KLALRPTSET VMYPMFALWV RSHTDLPMRF YQVVNTFRYE
    160 170 180 190 200
    TKHTRPLIRV REITTFKEAH TIHATASEAE EQVERAVEIY KEFFNSLGIP
    210 220 230 240 250
    YLITRRPPWD KFPGSEYTVA FDTLMPDGKT LQIGTVHNLG QTFARTFEIK
    260 270 280 290 300
    FETPEGDHEY VHQTCYGLSD RVIASVIAIH GDESGLCLPP DVAAHQVVIV
    310 320 330 340 350
    PIIFKKAAEE VMEACRELRS RLEAAGFRVH LDDRDIRAGR KYYEWEMRGV
    360 370 380 390 400
    PLRVEIGPRD LEKGAAVISR RDTGEKVTAD LQGIEETLRE LMKDILENLR
    410 420 430 440 450
    TRAWERMESE IREAETLEEA SRIVDEKRGI ISFMWCGEEE CGMDVEEKVR
    460 470 480
    VDILGIQEEG SGTCINCGRE APTGLTLPEH IS
    Length:482
    Mass (Da):55,806
    Last modified:January 1, 1998 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i57D81ED7E9496BAA
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE000666 Genomic DNA Translation: AAB85117.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    C69181

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAB85117; AAB85117; MTH_611

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mth:MTH_611

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|187420.15.peg.592

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000666 Genomic DNA Translation: AAB85117.1
    PIRiC69181

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1NJ1X-ray2.55A1-472[»]
    1NJ2X-ray3.11A1-472[»]
    1NJ5X-ray2.80A1-472[»]
    1NJ6X-ray2.85A1-472[»]
    SMRiO26708
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    IntActiO26708, 1 interactor
    MINTiO26708

    Genome annotation databases

    EnsemblBacteriaiAAB85117; AAB85117; MTH_611
    KEGGimth:MTH_611
    PATRICifig|187420.15.peg.592

    Phylogenomic databases

    HOGENOMiCLU_001882_4_2_2
    OMAiEVYWVTH

    Enzyme and pathway databases

    SABIO-RKiO26708

    Miscellaneous databases

    EvolutionaryTraceiO26708

    Family and domain databases

    CDDicd00778, ProRS_core_arch_euk, 1 hit
    Gene3Di3.30.110.30, 1 hit
    3.40.50.800, 1 hit
    HAMAPiMF_01571, Pro_tRNA_synth_type3, 1 hit
    InterProiView protein in InterPro
    IPR002314, aa-tRNA-synt_IIb
    IPR006195, aa-tRNA-synth_II
    IPR004154, Anticodon-bd
    IPR036621, Anticodon-bd_dom_sf
    IPR002316, Pro-tRNA-ligase_IIa
    IPR004499, Pro-tRNA-ligase_IIa_arc-type
    IPR016061, Pro-tRNA_ligase_II_C
    IPR017449, Pro-tRNA_synth_II
    IPR033721, ProRS_core_arch_euk
    PANTHERiPTHR43382, PTHR43382, 1 hit
    PfamiView protein in Pfam
    PF03129, HGTP_anticodon, 1 hit
    PF09180, ProRS-C_1, 1 hit
    PF00587, tRNA-synt_2b, 1 hit
    PRINTSiPR01046, TRNASYNTHPRO
    SMARTiView protein in SMART
    SM00946, ProRS-C_1, 1 hit
    SUPFAMiSSF64586, SSF64586, 1 hit
    TIGRFAMsiTIGR00408, proS_fam_I, 1 hit
    PROSITEiView protein in PROSITE
    PS50862, AA_TRNA_LIGASE_II, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYP_METTH
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O26708
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: June 2, 2021
    This is version 152 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
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