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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66ShikimateUniRule annotation1
Active sitei70Proton acceptorUniRule annotation1
Binding sitei91ShikimateUniRule annotation1
Binding sitei106ShikimateUniRule annotation1
Binding sitei224NADP; via carbonyl oxygenUniRule annotation1
Binding sitei226ShikimateUniRule annotation1
Binding sitei247NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 133NADPUniRule annotation5
Nucleotide bindingi153 – 158NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:MTH_242
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360631 – 283Shikimate dehydrogenase (NADP(+))Add BLAST283

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH242

Structurei

3D structure databases

ProteinModelPortaliO26344
SMRiO26344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01033 Archaea
COG0169 LUCA
KOiK00014
OMAiYHPILLP
OrthoDBiPOG093Z09ZR

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

O26344-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MITGKTHVTG IMGHPLGHSL SPIMHNAAFR SLGMDWIYVP FPVKPENLKA
60 70 80 90 100
AVEGLRALGV EGVNVTIPHK EAVLEYIDDI HGTAALIGAV NTLKFDDGTV
110 120 130 140 150
RGYNTDASGC LRALEEVTSV EGSSVLILGA GGAARACAFQ LAEKGASDIT
160 170 180 190 200
ILNRTPEKAR LLAEDMADKL GFEASYGGYE LIQGSVKSAD ILIDTTPVGM
210 220 230 240 250
HPHTDDRPLV GAELMHEGLV VHDLVYNPQR TVLLREAERA SAIPVSGIRM
260 270 280
LLYQGVEAFR IWTGRDPPLD VMEDALKRVL NRD
Length:283
Mass (Da):30,484
Last modified:December 1, 2000 - v2
Checksum:i3293E04F92FC0B89
GO

Sequence cautioni

The sequence AAB84748 differs from that shown. Reason: Frameshift at position 17.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB84748.1 Frameshift.
PIRiC69130

Genome annotation databases

EnsemblBacteriaiAAB84748; AAB84748; MTH_242
KEGGimth:MTH_242
PATRICifig|187420.15.peg.211

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB84748.1 Frameshift.
PIRiC69130

3D structure databases

ProteinModelPortaliO26344
SMRiO26344
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH242

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB84748; AAB84748; MTH_242
KEGGimth:MTH_242
PATRICifig|187420.15.peg.211

Phylogenomic databases

eggNOGiarCOG01033 Archaea
COG0169 LUCA
KOiK00014
OMAiYHPILLP
OrthoDBiPOG093Z09ZR

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00087

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_METTH
AccessioniPrimary (citable) accession number: O26344
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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